"Kada ku taba shakka cewa ƙaramin rukuni na masu tunani, ƴan ƙasa masu sadaukarwa na iya canza duniya.Hasali ma, ita kaɗai a wurin.”
Manufar Cureus ita ce ta canza tsarin wallafe-wallafen likita wanda ya daɗe, wanda ƙaddamar da bincike zai iya zama tsada, rikitarwa, da cin lokaci.
Platelet mai wadatar plasma/prp, farfadowar nama, kunna platelet, maganin haɓakar glucose, platelet, maganin haɓakawa.
Rubuta wannan labarin kamar: Harrison TE, Bowler J, Reeves K, et al.(Mayu 17, 2022) Tasirin glucose akan ƙidayar platelet da girma: abubuwan da ke haifar da maganin farfadowa.Magani 14 (5): e25081.doi:10.7759/cureus.25081
Platelet-rich plasma (PRP) da hypertonic glucose mafita ana amfani dasu don allura a cikin maganin farfadowa, wani lokacin tare.Ba a riga an ba da rahoton tasirin glucose na hypertonic akan jijiyar platelet da kunnawa ba.Mun gwada tasirin yawan adadin glucose a kan adadin platelet da erythrocyte, da kuma adadin sel a cikin PRP da jini duka (WB).An sami raguwa cikin sauri na ɓangaren adadin platelet tare da duk gaurayewar glucose gauraye da PRP ko jini gabaɗaya, daidai da ɓangaren lysis. Bayan minti na farko, adadin platelet ɗin ya tsaya tsayin daka, yana nuna saurin masaukin ragowar platelet zuwa matsananciyar hypertonicity (>2000 mOsm). Bayan minti na farko, adadin platelet ɗin ya tsaya tsayin daka, yana nuna saurin masaukin ragowar platelet zuwa matsananciyar hypertonicity (>2000 mOsm). После первой минуты количество тромбоцитов don эkstremalnoho (> 2000 mОsm) GIpertonusa. Bayan minti na farko, adadin platelet ɗin ya tsaya tsayin daka, yana nuna saurin masaukin ragowar platelet zuwa matsananciyar hypertonicity (>2000 mOsm).第一分钟后，血小板计数保持稳定，表明残余血小板迅速适应极端月> 2000 mOs.2000 mOsm）高渗状态。 После первой минуты количество тромбоцитов эkstremalnomu (> 2000 mОsm) гиperosmolyarnomu ssostoy. Bayan minti na farko, adadin platelet ɗin ya tsaya tsayin daka, yana nuna saurin karɓuwa na ragowar platelets zuwa matsananci (>2000 mOsm) yanayin hyperosmolar.Matsakaicin adadin glucose na 25% zuwa sama ya haifar da ƙaruwa mai yawa a cikin matsakaicin ƙarar platelet (MPV), yana nuna farkon matakin kunna platelet.Ana buƙatar ƙarin karatu don sanin ko ƙwayar platelet ko kunnawa ya faru kuma ko allurar glucose na hypertonic kadai ko a hade tare da PRP na iya ba da ƙarin fa'idar asibiti.
A cikin 1950s, likitan fiɗa na Amurka George Hackett ya gano cewa zai iya kawar da ciwon haɗin gwiwa da baya a yawancin marasa lafiya ta hanyar allurar maganin yaduwa a cikin tendons da ligaments.Gwajin da ya yi kan zomaye ya nuna cewa maganin, wanda ya kira proliferative therapy, ya sa jijiyoyi su kara girma da ƙarfafawa.Nazarin tarihi ya tabbatar da cewa ana samar da sabon collagen yayin wannan tsari [1].
A cikin ƴan shekarun farko na farko, an gwada hanyoyin rarraba iri daban-daban.A cikin shekarun 1990, yawancin masu yin aikin sun ɗauki babban taro na glucose a matsayin hanya mafi aminci kuma mafi inganci.Koyaya, tsarin aikin ya kasance ba a sani ba.
An gudanar da ƙananan binciken asibiti a cikin karni na 20 bayan aikin Hackett.Duk da haka, a cikin 2000s an sake sabunta sha'awa kuma an kammala gwaje-gwajen gwaji na asibiti da yawa don maganin ciwon baya [2], osteoarthritis na gwiwa [3], da epicondylitis na gefe [4].
Farfadowar nama yana buƙatar sa hannu na sel mai tushe.Don haka, babban taro na glucose dole ne ko ta yaya ya haifar da ƙaura, maimaitawa, da bambance bambancen sel masu tushe.Muna tsammanin cewa platelets na iya yin aiki a matsayin manzanni kuma yawan adadin glucose na iya haifar da platelet don saki cytokines da abubuwan girma, don haka inganta tsarin farfadowa, musamman ƙaurawar kwayar halitta zuwa wuraren da ke da yawan glucose.
Kunna platelet ko da yaushe yana gaba da karuwa a cikin calcium na cikin salula [5].Liu et al.a cikin 2008 ya nuna cewa matakan glucose masu girma suna haɓaka ayyukan tashoshi masu karɓa na wucin gadi na nau'in 6 (TRPC6) na canonical a cikin membrane na plasma, wanda ke haifar da kwararar ions na calcium cikin platelets [6].Wani binciken kuma ya nuna cewa fallasa yankin gefe na microtubule zuwa ions calcium yana haifar da annashuwa, faɗaɗawa, da kuma nakasar yanki na gefe, wanda hakan ke haifar da canji daga faifan diski zuwa mai siffa, wanda ke haifar da ma'aunin platelet (MPV) [7].
Hasashenmu a cikin wannan binciken shine bayyanar da platelets zuwa babban taro na glucose yana shafar yankin gefe na microtubule da yanayin cikin salula, yana haifar da karuwa a MPV.
Duk mahalarta sun sanya hannu kan takardar izini da aka ba da izini bayan an bayyana cikakkun bayanai game da binciken da kuma kafin su karɓi samfuran.A cikin wannan binciken, kawai samfurori na PRP tare da hematocrit fiye da 2% an yi amfani da su don haka za a iya haɗawa da erythrocyte (erythrocyte) ƙidaya kuma yana nufin ƙarar ƙwayar jini na jini (MCV) don kwatantawa.
An gudanar da binciken a matakai hudu, kashi na farko shine PRP kuma sauran sassan jini ne (Table 1).Kamar yadda aka bayyana a baya [8], duk dangi na centrifugal (RCF, g-force) an ƙididdige su daga tsakiya (Rmid, a cikin cm) na ginshiƙin jini a cikin sirinji na centrifugal.Mun zaɓi yin amfani da MPV a matsayin alama na wayar da kan platelet da ƙidaya platelet a matsayin mai nuna yuwuwar yuwuwar yuwuwar platelet lysis, waɗanda za a iya auna su cikin sauƙi akan daidaitattun masu nazarin ilimin jini.
A cikin kashi na farko, masu aikin sa kai na 47 sun ba da gudummawar samfuran jini-tubu ɗaya na ethylenediaminetetraacetic acid (EDTA) da kuma samfurin jini na PRP guda ɗaya (wanda aka lalata da sodium citrate (NaCl, 3%)) (Table 1).Sanya rocker a cikin bututu nan da nan.An yi cikakken ƙididdigar jini (CBC) akan samfuran EDTA a cikin sau uku, kuma an bincika samfuran NaCl a cikin sau uku don nazarin CBC, sannan an shirya PRP ta hanyoyi daban-daban da aka bayyana a sama [8].Duk samfuran PRP an shirya su ta hanyar centrifugation a 900-1000 g.Mix kowane samfurin PRP akan mahaɗin vortex don 5-10 seconds, sa'an nan kuma raba 0.5 ml aliquots guda biyar a cikin tubes.
Don kimanta tasirin tasirin platelet akan haɓakar glucose mai girma, daidaitattun adadin (0.5 ml) na 0%, 5%, 12.5%, 25%, da 50% glucose a cikin ruwa an haɗe su tare da samfuran platelet don samun 0%, 2.5% 6.25%, 12.5% ​​da 25% na gwajin gwaji na mintuna 1 na bututun glucose a cikin bututun sha.An yi nazarin TAC na kowane cakuda a cikin sau uku bayan 15 min.Ƙididdigar platelet (PLT), ƙidayar RBC, MCV, da MPV an ƙididdige su ga kowane bututu, kuma ana ƙididdige ma'anar ƙididdigar platelet, ƙididdigar RBC, MCV, da MPV don duk samfuran PRP.
Bayan an kammala kashi na farko na tattara bayanai, mun lura da karuwa mai yawa a cikin adadin platelet a cikin PRP platelets bayan ƙara D50W.PRP ba dole ba ne su wakilci dukkan platelets a cikin jini, kuma matsakaicin PRP ya bambanta da matsakaicin WB.Saboda haka, mun yanke shawarar gudanar da gwajin kashi na biyu na tasirin ƙara D50W zuwa jini gaba ɗaya.
Don zagaye na biyu, mun zaɓi girman samfurin 30 bisa ga sakamakon daga jerin farko, kamar yadda aka bayyana a cikin sashin nazari.A cikin wannan jerin, masu sa kai 20 sun ba da gudummawar samfuran jini (Table 1).An jawo cikakken jini (1.8 ml) a cikin sirinji na 3 ml kuma an shafe shi da 0.2 ml 40% NaCl.An haxa dukkan sirinji na jini na daƙiƙa biyar tare da mahaɗar vortex kuma an bincika CBC sau uku.Bayan bincike, an ƙara jinin anticoagulated zuwa 2 ml na 50% glucose a cikin sirinji na 5 ml (nauyin glucose na ƙarshe ya kasance kusan 25% (D25) kuma an sanya shi a cikin bututun girgiza don mintuna 30. Bayan mintuna 30, an bincika D25/CBC a cikin allurar WB a cikin nau'in syringes. V, da MPV an ƙididdige su don kowane samfurin kafin da bayan ƙara glucose.
Saboda platelets a cikin jini gabaɗaya ana fallasa su ga glucose na hypertonic yayin maganin glucose na yaduwa saboda ƙarancin allura, kuma ba a saba haɗa PRP tare da glucose na hypertonic kafin allura ba, mun yanke shawarar yin nazarin glucose na hypertonic tare da WB a Sashe na 1. Mataki na uku da na huɗu.A kowane mataki, 20 masu aikin sa kai sun ba da 7-8 ml na ACD-A (acid dauke da trisodium citrate (22.0 g / l), citric acid (8.0 g / l) da glucose (24.5 g / l), bayani dextrose citrate) don maganin rigakafi na jini (Table 1).Ganyayyaki na glucose sama da 12.5% ​​kawai aka yi amfani da su don tantance adadin kofa da ke da alaƙa da haɓakar MPV.A mataki na uku, an sanya 1 ml na jini a cikin bututun gwaji.Sa'an nan kuma haxa jinin a kan mahaɗin vortex na daƙiƙa 10 ta hanyar ƙara 1 ml na 30% glucose, 40% glucose, ko 50% glucose a cikin bututu don samun ƙwayar glucose na ƙarshe na 15%, 20%, da 25%, bi da bi.An bincika samfuran jinin glucose don CBC nan da nan bayan haɗuwa kuma a maimaita kowane minti biyu na mintuna 30.
A lokacin haɗuwa na farko, ƙari na 1: 1 hypertonic glucose da WB ko PRP yana fallasa platelet zuwa taro sama da 25% na daƙiƙa da yawa.A mataki na huɗu, don kimanta tasirin glucose na hypertonic tare da ƙaramin adadin farko na farko da gwada iyakar tasirin glucose, mun ƙara ƙaramin adadin jini zuwa D25W ko D50W.Sanya 1 ml na D25W ko D50W a cikin bututu kuma ƙara 0.2 ml na WB yayin jujjuya samfurin na daƙiƙa 10.A cikin waɗannan lokuta, jinin yana nunawa ga glucose a wani taro kusan 20% sama da maida hankali na ƙarshe, maimakon 50% sama da taro na ƙarshe kamar a cikin Mataki na 3, wanda ya haifar da ƙaddamarwar glucose na ƙarshe na 20.8% da 41.6%.An yi nazarin samfurori masu gauraya a lokaci guda kamar a mataki na 3.
A cikin matakin farko na kowane jerin dilution na glucose, an ɗauki samfurori 30 saboda wannan shine girman samfurin da ya dace don binciken matukin jirgi [9].A ƙarshen kowane lokaci (ciki har da kashi na farko), ƙididdige cancantar girman samfurin ta amfani da dabarar da aka yi amfani da ita don ƙayyade girman samfurin da ake buƙata don ƙididdige ma'anar madaidaicin sakamako mai ci gaba a cikin yawan jama'a.Formula n = Z2 x SD2 / E2.A cikin wannan ma'auni, Z shine makin Z, SD shine madaidaicin karkata, kuma E shine kuskuren da ake so [10].Alfa ɗin mu shine 0.05, wanda yayi daidai da ƙimar Z na 1.96, kuma muna tsammanin kuskuren 5 (a cikin kashi).Don haka muna warware n = (1.962 x SD2)/52.Sakamakon ya nuna cewa girman samfurin da ake buƙata don kowane mataki ya yi ƙasa da ainihin adadin da aka tattara.
A cikin lokuta 1, 3 da 4 ta yin amfani da maida hankali na glucose fiye da ɗaya, an bincika tasirin matakan glucose daban-daban ta hanyar kwatanta canjin juzu'i tsakanin lokaci 0 da kowane lokaci na gaba (lokaci na 1 a mintuna 15, lokaci na 3 a cikin mintuna 15).da hudu a 15 seconds, sa'an nan kowane minti biyu.) Canja rates na kowane lokaci lokaci an kwatanta ta amfani da Mann-Whitney U-test saboda bayanai ba su bi wani al'ada rarraba kamar yadda ƙaddara ta Shapiro-Wilk al'ada gwajin.Tun da an yi nazarin 1-to-1 na ƙungiyoyi da yawa (biyar) a cikin matakai na farko, na uku da na hudu (biyar a duka), an yi gyaran gyaran Bonferroni don daidaita darajar alpha da ake so zuwa ≤0.01 amma ba ≤0.05 ba.
Rage ƙididdiga na platelet tare da duk abubuwan da ke tattare da hypertonic dextrose da karuwa a MPV a cikin PRP platelets a> 12.5% ​​dextrose maida hankali: PRP platelet ƙidaya ya tashi daga sau ɗaya zuwa sau biyar maida hankali idan aka kwatanta da tushen duka jini, ya bambanta ta hanyar (ba a kwatanta ba). Rage ƙididdiga na platelet tare da duk matakan hypertonic dextrose da karuwa a MPV a cikin PRP platelets a> 12.5% ​​dextrose maida hankali: PRP platelet ƙidaya ya tashi daga sau ɗaya zuwa sau biyar maida hankali idan aka kwatanta da tushen tushen duka jini, ya bambanta ta hanyar (ba a kwatanta ba). Уменьшение Уменьшение количества тромбоцитов при всех концентрациях гипертонической рации декстрозы > 12.5%: количество тромбоцитов PRP увеличилось в 1-5 раз по сравнению с исходной метода (ba kyauta ba). Rage yawan adadin platelet a duk matakan hypertonic dextrose da kuma ƙara MPV a cikin PRP platelets a> 12.5% ​​dextrose maida hankali: PRP platelet ƙidaya ya karu sau 1-5 idan aka kwatanta da tushen dukan jini, dangane da hanyar (ba a nuna ba). ).在> Kashi 12.5%相比，PRP 血小板计数从浓度的1 倍上升到5 倍，因方法而异（未描述）。 A> 12.5% ​​glucose maida hankali, babban taro na glucose yana rage adadin jini, PRP jini MPV yana ƙaruwa: idan aka kwatanta da 与基线全血, adadin jinin PRP yana ƙaruwa daga 1 zuwa 5 sau na maida hankali (ba a bayyana ba). При концентрациях глюкозы > 12.5% ​​все концентрации мбоцитах PRP: количество тромбоцитов PRP увеличивалось от 1- до 5-кратныh концентраций ой крови, в зависимости от метода (не описано ). A cikin matakan glucose> 12.5%, duk matakan glucose na hauhawar jini sun rage adadin platelet kuma ƙara MPV a cikin platelet PRP: PRP platelet ƙidaya ya karu 1- zuwa 5-nnki idan aka kwatanta da tushen jigon jini duka, dangane da hanyar (kamar yadda aka bayyana).Hoto 1 yana nuna cewa adadin platelet ya ragu da kusan 75% bayan dilution a cikin ruwa da kuma ta 20-30% bayan mintuna 15 na dilution tare da nau'ikan glucose daban-daban idan aka kwatanta da PRP na asali da 1: 1 dilution wanda aka daidaita don ƙarar (1-k1 tare da gyaran ƙara).k -1 kiwo).1 kiwo).
An bayyana adadin ƙwayoyin sel a cikin kowane dilution a matsayin ɗan juzu'i na lambar asali kafin dilution.
MPV ya ragu kaɗan yayin samar da PRP, ba tare da ƙarin canji a cikin adadin dilution zuwa 12.5% ​​a cikin ruwa ko glucose (ciki har da 25% PRP cakuda glucose) kuma ya karu da fiye da 20% bayan dilution a cikin 50% glucose bayani (Fig. .2).).Sabanin haka, erythrocytes ba su nuna wani gagarumin canji a cikin girma a kowane dilution ban da H2O.
Matsakaicin adadin sel a cikin kowane dilution ana bayyana shi azaman kashi na ainihin ƙarar kafin dilution.
Irin wannan raguwar raguwar platelet da karuwa a cikin CVR an lura da shi a cikin BC wanda aka fallasa zuwa glucose 50% (don ƙirƙira tare da 25% glucose).Tebura 2 yana kwatanta lambobin salula da adadin tantanin halitta a cikin dukkanin jini da aka diluted a cikin 50% dextrose tare da lokaci 1 PRP bayanan diluted a cikin 50% dextrose.Canje-canje a cikin ƙididdigar RBC da RBC MCV ba a bayyane suke ba kuma ba su ne abin da ya fi mayar da hankali ga hankalinmu ba.
SD = daidaitattun daidaituwa, MD = ma'anar bambanci tsakanin ƙungiyoyi, SE = daidaitattun bambancin ma'ana, RBC = erythrocytes, PLT = platelet, PRP = plasma mai arziki, WB = dukan jini
Bayan ƙara D50W zuwa WB, adadin dilution-daidaita asarar platelet shine 7.7% (310 ± 73 vs. 286 ± 96) idan aka kwatanta da 17.8% don dilution PRP a D50W (664 ± 348 vs. 544 ± 277).MPV WB ya karu da 16.8% (daga 10.1 ± 0.5 zuwa 11.8 ± 0.6), yayin da MPV PRP ya karu da 26% (9.2 ± 0.8 vs. 11.6 ± 0. 7). Kodayake ma'anar bambance-bambance a cikin raguwar ƙididdigar platelet da karuwar MPV sun kasance da yawa tare da PRP, sauye-sauye a cikin raguwar adadin platelet a cikin WB sun kasance kusan mahimmanci (310 ± 73 zuwa 286 ± 96 (-7.7%); p = .06) kuma karuwa a MPV ya kasance mai mahimmanci (10.1 ± 0.5 zuwa 11.8) <+ 0.8). Kodayake ma'anar bambance-bambance a cikin raguwar ƙididdigar platelet da karuwar MPV sun kasance da yawa tare da PRP, sauye-sauye a cikin raguwar adadin platelet a cikin WB sun kasance kusan mahimmanci (310 ± 73 zuwa 286 ± 96 (-7.7%); p = .06) kuma karuwa a MPV ya kasance mai mahimmanci (10.1 ± 0.5 zuwa 11.8) <+ 0.8).Kodayake ma'anar bambance-bambance a cikin raguwar ƙididdigar platelet da haɓakar CVR sun kasance mafi girma tare da PRP, canje-canje a cikin ƙidayar platelet a cikin WB sun kasance kusan mahimmanci (310 ± 73 zuwa 286 ± 96 (-7.7%); p = 0.06).увеличение MPV было значительным (от 10,1 ± 0,5 da 11,8 ± 0,6 (+16,8) p <0,001). karuwa a cikin MPV yana da mahimmanci (daga 10.1 ± 0.5 zuwa 11.8 ± 0.6 (+16.8) p <0.001).尽管PRP 在血小板计数减少和MPV 增加方面的平均差异显着更大，但WB 内血小显着更大，但WB显着的（310 ± 73 至286 ± 96 (-7.7%)；p = .06）和MPV 的增加是显着的（10.1 ± 0.5 到11.8) <+0.8尽管 PRP 在 血小板 计数 和 和 增加 方面 的 平均 差异 显着 大乎 是 显着 的 （（(310 ± 73 至 286 ± 96 (-7.7%) ； p = .06）和MPV 的增加是显着的（ 10.5 .001).Canjin raguwar ƙididdigar platelet a cikin WB ya kusan kusan mahimmanci (daga 310 ± 73 zuwa 286 ± 96 (-7.7%); p = 0.06), kodayake PRP yana da ma'anar bambance-bambance mafi girma a cikin raguwar adadin platelet da haɓaka MPV.kuma karuwar MPV ya kasance mai mahimmanci.(от 10,1 ± 0,5 da 11,8 ± 0,6 (+16,8) р <0,001). (daga 10.1 ± 0.5 zuwa 11.8 ± 0.6 (+16.8) p <0.001).
Ana buƙatar maida hankali na ƙarshe na 20% glucose don ganin babban canji a MPV, amma canjin MPV ya fi bayyana a matakin ƙarshe na 25%.Asarar platelet ya daidaita bayan faɗuwar farko.Mun lura da raguwar kaifi na farko a cikin CVR, duk da haka, an sake dawo da CVR da sauri a cikin 25% na glucose na ƙarshe, wanda ya fi girma fiye da matakan CVR da aka lura a matakin glucose na ƙarshe na 20% da 15% (Fig. 3 da hagu na Table 3; akwatunan shaded).nuna p-darajar ≤ alpha tare da gyaran Bonferroni na 0.01).Hakanan an sami raguwar kaifi na farko a cikin adadin PLT, wanda aka lura a farkon lokacin 0-15 s, sannan ya kasance barga (daga 15 s zuwa 30 min; hagu na tebur 4).
Ƙara yawan adadin glucose a cikin jini gaba ɗaya ya haifar da raguwa cikin sauri a cikin MPV tare da farfadowa mai dogara da hankali fiye da 20%.Labarin yana nuna yawan adadin glucose bayan dilution.D15, D20 da D25 an yi su a cikin dilution 1:1.D21 da D41 an yi su a 1:5 dilution.
Tebu 4 yana nuna canjin adadin platelet lokacin da aka diluted cikin glucose na hypertonic.Mun lura da dangantakar da ke dogara da kashi tsakanin raguwar sauri a cikin lambobin PLT a 1: 1 dilution da kuma a 1: 5 dilution.Kwatanta 1: 1 dilutions a matsayin ƙungiya ɗaya tare da 1: 5 dilutions, ƙungiyar 1: 1 ta sami raguwa nan da nan a cikin adadin platelet kasa da 1: 5 rukuni 66 ± 48,000 (23%) da 99 ± 69,000 (37%)., p = 0.014) a cikin rukunin 1:5.Bayan faɗuwar farko a ma'aunin farko, platelet ɗin yana ƙididdige adadin adadin glucose ya daidaita (Fig. 4).
Lokacin da aka ƙara duka jini zuwa glucose a cikin rabo na 1: 1, adadin platelet ɗin yana raguwa da kusan 25%.Duk da haka, lokacin da aka ƙara jini duka a cikin rabo na 1: 5, raguwa ya fi girma - kimanin 50%.
41% glucose ya karu da sauri MPV kuma fiye da ban mamaki fiye da 25% ko 21%.Ana nuna sakamakon MPV a cikin Hoto 3. A duk sauran dilutions, ba a sami raguwa na farko a MPV ba bayan ƙara 50% glucose.Lokacin amfani da 25% glucose (glucose maida hankali 20.8% a ƙarshen dilution), canjin MPV ya kasance daidai da canjin 20% glucose a 1: 1 dilution (Fig. 3).Kodayake canje-canje a cikin MPV sun fara girma a 41% gauraye maida hankali fiye da a 25%, bambancin MPV tsakanin 41% da 25% bayan mintuna 16 ba shi da mahimmanci (Table 3, dama).Hakanan yana da ban sha'awa cewa 25% glucose ya karu MPV fiye da yadda ya kamata fiye da 20.8%.
Wannan binciken in vitro ya ɗan tabbatar da hasashen mu. Ya nuna yuwuwar haɓakar platelet lysis ta hanyar dextrose admixture, saurin masaukin platelet zuwa matsananciyar hypertonicity, da haɓakar girma a cikin MPV don amsa> 25% taro na hypertonic dextrose. Ya nuna yuwuwar haɓakar platelet lysis ta hanyar dextrose admixture, saurin masaukin platelet zuwa matsananciyar hypertonicity, da haɓakar girma a cikin MPV don amsa> 25% taro na hypertonic dextrose. Оn pokazal pokazal potentsyalnыy chess trombotsytov гипертонуса и значительное повышение MPV в ответ на гипертоническую концентрацию декстрозы > 25%. Ya nuna yuwuwar haɓakar platelet lysis tare da dextrose, saurin masaukin platelet zuwa matsananciyar hypertonicity, da haɓakar girma a cikin MPV don amsa matakan dextrose hypertonic> 25%.它显示出通过葡萄糖混合物潜在的部分血小板溶解，血小板快速适应极端们应>不可帔>25%的高渗葡萄糖时MPV 显着上升。它 显示 出 通过 葡萄糖 潜在 的 部分 一小板浓度 高渗 葡萄糖 时 时 mpv 显着….. Он показывет потильный чаstychnыy у гипертонусу и значительное увеличение MPV в ответ на концентрацию гипертонической глюкозы > 25%. Yana nuna yuwuwar rarrabuwar platelet ta hanyar gaurayawan glucose, saurin daidaitawar platelet zuwa matsananciyar hypertonicity, da haɓakar girma a cikin MPV don amsa glucose hypertonic> 25%.Ƙaruwar farko ta kasance mafi girma a 41.6% bayyanar glucose, amma karuwa a MPV ya kusanta 25% bayyanar glucose kamar minti 20 bayan bayyanar.
Matsakaicin adadin platelet yana shafar glucose.Mun lura cewa adadin PLT ya ragu a duk dilutions na glucose.Matsakaicin raguwar adadin platelet a cikin H2O (0%) dilutions na jerin PRP na iya haɗawa da osmotic lysis.A madadin, wannan na iya zama wani kayan tarihi da ke haifar da tarin platelet, amma wannan ya bambanta da rashin canjin MPV a wannan dilution.Wannan binciken yana nufin cewa wasu platelets suna da matukar damuwa ga hypoosmolarity.
A cikin duk 1: 1 dilutions na glucose, adadin PLT ya ragu da 20-30%, har ma da D5W (hypotonic a 252 mOsm), wanda zai iya nuna takamaiman tasirin glucose ba tare da osmotic ba, tunda duka PLT da MPV sun kasance ba su canzawa a haɓaka sau uku a cikin maida hankali.glucose.daga D5W zuwa D25W.A gaskiya ma, ƙididdigar PLT sun kasance suna ƙara dan kadan tare da ƙara yawan osmolarity.
Ragewar PLT tsakanin 1: 1 da 1: 5 dilutions yana nufin cewa tasirin rushewar ya dogara ne akan matakin glucose na farko da na ƙarshe.Idan ya dogara ne kawai akan ƙaddamarwar farko, to, mutum zai yi tsammanin ganin bambanci a cikin raguwar PLT tsakanin 1: 1 maida hankali.Amma ba mu yi ba.Idan tasirin lysis ya dogara ne kawai akan ƙaddamarwar glucose na ƙarshe, to, ba ma tsammanin babban bambanci tsakanin dilution 20% 1: 1 da dilution 20.8% 1: 5.Amma duk da haka mun yi shi.
Idan asarar platelet ya faru saboda lysis na platelet, an kafa wani ɓangaren lysate, bayan haka an saki cytokines da abubuwan girma a cikin yanayin waje.Yawancin karatu sun nuna cewa platelet lysate yana kusan tasiri kamar PRP a matsayin maganin yaduwa [11].An nuna PRP kanta a matsayin mafita mai mahimmanci don maganin yaduwa [12-14].
platelets marasa aiki suna yawo a cikin nau'in faifai da aka ƙarfafa tare da tsarin ciki da yawa.A lokacin kunnawa, suna ɗaukar siffar mai siffar zobe ko amoeba, wanda ke haifar da karuwa a girma.Ƙara ƙarar ƙarar yana buƙatar karuwa a sararin samaniya, wanda shine sakamakon ƙaddamar da tsarin buɗaɗɗen tubule (OCS) da ƙari na exocytic granules zuwa membrane.Ya rage don tantance ko haɓakar MPV da ke haifar da glucose na hypertonic ya ƙunshi ɗaya ko duka waɗannan hanyoyin, amma idan na ƙarshe, haɓakar MPV zai nuna lalata.
Wannan binciken ya nuna cewa fallasa yawan adadin glucose akan PRP ko duka platelets na jini ya haifar da karuwa a cikin MPV a cikin mintuna 15 tare da maida hankali na glucose na 25% da 41.6%, bi da bi.
Ƙaruwa a cikin platelet MPV na iya kasancewa saboda ƙaddamar da tangles na microtubule da ke kewaye da su don mayar da martani ga kwararar calcium.Liu et al.An nuna glucose don daidaita ƙwayar calcium ta hanyar tashar platelet TRPC6 [6].Hasashen mu shine glucose yana haifar da shakatawa na tangles na microtubule, yana haifar da haɓakar MPV da faranti da / ko kunnawa.Koyaya, idan aka yi la'akari da sakamakonmu, wannan ɓangaren labarin ne kawai.A cikin gwaje-gwajenmu, babu maida hankali a ƙasa D25W da ya haifar da karuwa a MPV.Ganin cewa ba mu gwada bayyanar da adadin glucose tsakanin 12.5% ​​da 25% ba, sakamakon mu na lokaci na 1 yana nuna cewa za a iya samun kofa a cikin wannan kewayon yawan glucose wanda ke haifar da karuwa a MPV.Karin gwaje-gwaje a matakai na 3 da 4 sun nuna cewa 20-25% glucose ya bayyana a matsayin kofa na wannan, amma har yanzu ba a san dalilin da ya sa ba.
Mun kuma lura da raguwar ~9% a MPV bayan centrifugation.Ba a fayyace ko wannan raguwar MPV ya kasance saboda girma da yawa kuma masu yawa platelets sun makale a cikin layin RBC na centrifuge.Wannan abin lura na iya zama da mahimmanci ga likitocin asibiti saboda yana iya nuna cewa PRP platelets ƙananan ƙananan ƙananan ƙwayoyin platelets ne.
A cikin binciken da ya gabata, mun nuna cewa shirye-shiryen PRP ta hanyoyin hannu ba shi da tsada [8].Idan glucose yana wayar da kan nama platelets ko PRP, yana sa su zama masu sauƙi ga kunnawa, ko kuma idan an samar da PRP tare da kaddarorin lysate, wannan na iya haɓaka haɓakawa kuma yana rage buƙatar magani.Saboda haka, haɗin PRP da glucose mai yawan gaske na iya zama mafi tsada-tasiri fiye da PRP ko glucose kadai.
Nazarinmu yana da kasawa da yawa.Na farko, muna amfani da PRP da aka samo daga hanyoyi daban-daban.Wannan na iya haifar da sakamako masu karo da juna.Na biyu, ba mu iya yin nazarin sinadarai na kowane samfurin mu don ƙarin tantance ko kunna platelet ya faru ba.Muna so mu auna P-selectin, platelet factor 4, monocytic platelet aggregates, ko wasu alamomin kunna platelet don ƙarin fahimtar digiri ko kasancewar lalatawar alpha granule, amma wannan ya wuce iyakar wannan binciken.Na uku, ba mu iya tabbatarwa ta hanyar microscopy na lantarki ko wasu hanyoyin cewa haɓakar MPV a cikin platelet masu fallasa glucose ya kasance saboda tasirin microtubule tangles.
Haɗuwa da WB ko PRP tare da 25% glucose sun karu MPV, yana nuna alamar fara kunna platelet, kodayake wannan binciken bai nuna ci gaban haɓakawa ko raguwa ba.Haɗin glucose na hypertonic ya haifar da asarar platelet, mai yiwuwa yana wakiltar tasirin lytic.Kunna wani bangare ko lysis na platelet na iya haifar da farfadowar nama bayan allurar platelet.Ba a bayyana irin sakamakon asibiti waɗannan canje-canjen na iya haifar da su ba.Ƙarin karatu sun nuna ƙarin ingantattun ma'auni na kunnawa ko lysis kuma sun kimanta tasirin asibiti daban-daban na gaurayawan glucose na hypertonic tare da WB ko PRP.
Glucose proliferative far ne mai sauƙi kuma maras tsada farfadowa na farfadowa wanda ke fadada hanzari da kuma tallafawa bincike na asibiti.Wannan binciken yana ba da shawarar tsarin ilimin lissafin jiki wanda, idan an tabbatar da shi, zai iya taimaka mana mu fahimci wani ɓangare na tsarin farfadowa na farfadowa.
Ilimin Halittu da Ilimin Lafiya a Jami'ar Missouri, Makarantar Magunguna ta Kansas City, Kansas City, Amurka
Abubuwan Dan Adam: Duk mahalarta wannan binciken sun ba da izini ko ba su ba da izini ba.Ƙungiyar Ƙasa ta Duniya don Magungunan salula ta ba da izinin ICMS-2017-003.An amince da ƙa'idar mai zuwa don ƙarin amfani da Hukumar Binciken Cibiyoyi ta Ƙungiyar Ƙasa ta Duniya don Magungunan Salon salula: Take: Ƙididdigar yawan yawan magungunan plasma mai wadataccen ƙwayar cuta bisa tushen ƙidayar platelet CBC.Abubuwan Dabbobi: Duk marubuta sun tabbatar da cewa babu dabbobi ko kyallen takarda da ke da hannu a cikin wannan binciken.Rikice-rikice na Sha'awa: Dangane da Form Bayyanar ICMJE Uniform, duk mawallafa suna bayyana waɗannan abubuwa masu zuwa: Biyan kuɗi / bayanin sabis: Duk marubutan sun bayyana cewa ba su sami tallafin kuɗi daga kowace ƙungiya don aikin da aka ƙaddamar ba.Dangantakar Kuɗi: Duk marubutan sun bayyana cewa ba su da alaƙar kuɗi a halin yanzu ko a cikin shekaru uku da suka gabata tare da kowace ƙungiyar da ke da sha'awar aikin da aka ƙaddamar.Sauran Dangantaka: Duk mawallafa sun bayyana cewa babu wasu alaƙa ko ayyukan da zasu iya shafar aikin da aka ƙaddamar.
Harrison TE, Bowler J, Reeves K et al.(Mayu 17, 2022) Tasirin glucose akan ƙidayar platelet da girma: abubuwan da ke haifar da maganin farfadowa.Magani 14 (5): e25081.doi:10.7759/cureus.25081
© Haƙƙin mallaka 2022 Harrison et al.Wannan labarin buɗe ido ne wanda aka rarraba ƙarƙashin sharuɗɗan lasisin Haɗin Haɗin Halittun Halittu CC-BY 4.0.An ba da izinin amfani mara iyaka, rarrabawa, da haɓakawa a kowace matsakaici, in dai an ƙididdige ainihin marubucin da tushen.