Ua hoʻomohala ʻia kahi huila static inline hou i hoʻolālā ʻia e hoʻokō i nā koi koʻikoʻi o nā ʻōnaehana chromatography wai kiʻekiʻe (HPLC) a me nā ʻōnaehana wai kiʻekiʻe kiʻekiʻe (HPLC a me UHPLC).ʻO ka hoʻohui maikaʻi ʻole ʻana o ʻelua a ʻoi aku ka nui o nā pae kelepona hiki ke hopena i kahi ratio hōʻailona-a-noise kiʻekiʻe, e hōʻemi ana i ka naʻau.ʻO ka hui pū ʻana o nā wai ʻelua a ʻoi aʻe paha me ka liʻiliʻi o ka leo kūloko a me nā ʻano kino o kahi hui static e hōʻike ana i ke kūlana kiʻekiʻe loa o kahi hui static kūpono.Hoʻokō ka mea hui static hou i kēia me ka hoʻohana ʻana i ka ʻenehana paʻi 3D hou e hana i kahi ʻano 3D kūʻokoʻa e hāʻawi ana i ka hoʻohuihui static hydrodynamic i hoʻomaikaʻi ʻia me ka haʻahaʻa haʻahaʻa kiʻekiʻe o ka hawewe sine kumu no kēlā me kēia leo kūloko o ka hui.ʻO ka hoʻohana ʻana i ka 1/3 o ka leo kūloko o kahi mixer maʻamau e hōʻemi i ka nalu sine kumu ma 98%.ʻO ka hui hui pū ʻia nā kahawai kahe 3D i hoʻopili ʻia me nā ʻāpana ʻāpana like ʻole a me nā lōʻihi o ke ala i ka wā e hele ai ka wai i nā geometries 3D paʻakikī.ʻO ka hui pū ʻana me nā ala kahe nui, i hui pū ʻia me ka haunaele kūloko a me nā eddies, ka hopena i ka hui ʻana ma nā unahi micro, meso a me macro.Hoʻolālā ʻia kēia mea hui ʻokoʻa me ka hoʻohana ʻana i nā simulation fluid dynamics (CFD).Hōʻike ka ʻikepili hoʻāʻo i hōʻike ʻia e hoʻokō ʻia ka hui ʻana me ka liʻiliʻi o ka leo kūloko.
No nā makahiki ʻoi aku ma mua o 30, ua hoʻohana ʻia ka chromatography wai i nā ʻoihana he nui, me nā lāʻau lapaʻau, pesticides, pale kaiapuni, forensics, a me ka loiloi kemika.ʻO ka hiki ke ana i nā ʻāpana o ka miliona a i ʻole he mea koʻikoʻi i ka hoʻomohala ʻenehana i kekahi ʻoihana.ʻO ka maikaʻi ʻole o ka hui ʻana e alakaʻi i ka ratio hōʻailona-a-noise maikaʻi ʻole, he mea hoʻonāukiuki i ke kaiāulu chromatography e pili ana i nā palena ʻike a me ka naʻau.I ka hui ʻana i ʻelua mau mea hoʻoheheʻe HPLC, pono i kekahi manawa ke hoʻoikaika i ka hui ʻana ma nā ʻano o waho e homogenize i nā mea hoʻoheheʻe ʻelua no ka mea ʻaʻole hui maikaʻi kekahi mau solvents.Inā ʻaʻole i hui pono ʻia nā mea hoʻoheheʻe, hiki ke hoʻohaʻahaʻa ʻia ka HPLC chromatogram, e hōʻike ana iā ia iho me ke ʻano o ka leo kumu nui a/a i ʻole ke ʻano o ka piko.Me ka maikaʻi ʻole o ka hui ʻana, ʻike ʻia ka walaʻau kumu ma ke ʻano he hawewe sine (piʻi a hāʻule) o ka hōʻailona ʻike i ka manawa.I ka manawa like, hiki i ka hui ʻana maikaʻi ke alakaʻi i ka hoʻonui ʻana a me ka asymmetric peaks, e hōʻemi ana i ka hana analytical, ʻano kiʻekiʻe, a me ka hoʻonā kiʻekiʻe.Ua ʻike ka ʻoihana ʻo nā mea huila static in-line a me ka tee kahi ala e hoʻomaikaʻi ai i kēia mau palena a hiki i nā mea hoʻohana ke hoʻokō i nā palena ʻike haʻahaʻa (sensitivities).Hoʻohui ka mixer static maikaʻi i nā pōmaikaʻi o ka maikaʻi o ka hui ʻana, haʻahaʻa make haʻahaʻa a me ka haʻahaʻa haʻahaʻa haʻahaʻa me ka liʻiliʻi liʻiliʻi a me ka nui o ka ʻōnaehana.Eia kekahi, i ka lilo ʻana o ka hoʻopaʻa ʻana i mea paʻakikī, pono nā mea loiloi e hoʻohana mau i nā mea hoʻoheheʻe polar a paʻakikī hoʻi e hui pū.ʻO ke ʻano o ka hui ʻana ʻoi aku ka pono no ka hoʻāʻo ʻana i ka wā e hiki mai ana, e hoʻonui hou i ka pono no ka hoʻolālā mixer kiʻekiʻe a me ka hana.
Ua hoʻomohala hou ʻo Mott i kahi ʻano hou o nā mixer static inline PerfectPeakTM i hoʻopaʻa ʻia me ʻekolu mau puke i loko: 30 µl, 60 µl a me 90 µl.Hoʻopili kēia mau nui i ka laulā o nā puke a me nā ʻano hui ʻana e pono ai no ka hapa nui o nā hoʻāʻo HPLC kahi e pono ai ka hui ʻana a me ka haʻahaʻa haʻahaʻa.ʻO nā hiʻohiʻona ʻekolu a pau he 0.5 ″ ka anawaena a hāʻawi i ka hana alakaʻi alakaʻi ʻoihana ma kahi hoʻolālā paʻa.Hana ʻia lākou me 316L stainless steel, passivated no ka inertness, akā ʻo ka titanium a me nā mea hoʻoheheʻe ʻē aʻe a me nā kemika inert metala i loaʻa pū kekahi.Loaʻa i kēia mau mea hoʻohui i ke kaomi hana kiʻekiʻe a hiki i 20,000 psi.Ma ka fig.He kiʻi ʻo 1a o kahi huila static 60 µl Mott i hoʻolālā ʻia e hāʻawi i ka maikaʻi o ka hui ʻana me ka hoʻohana ʻana i ka leo kūloko liʻiliʻi ma mua o nā mea hui maʻamau o kēia ʻano.Ke hoʻohana nei kēia hoʻolālā mixer static hou i ka ʻenehana hana hoʻohui hou e hana i kahi ʻano 3D kūʻokoʻa e hoʻohana ana i ka kahe o loko ma mua o nā mea hui i hoʻohana ʻia i kēia manawa i ka ʻoihana chromatography e hoʻokō i ka hui ʻana.ʻO ia mau mea hui pū ʻia nā kahawai kahe ʻekolu-dimensional me nā ʻāpana keʻa like ʻole a me nā lōʻihi ala like ʻole i ka wā e hele ai ka wai i nā pale geometric paʻakikī i loko.Ma ka fig.Hōʻike ka Figure 1b i kahi kiʻi kiʻi o ka mea hui hou, e hoʻohana ana i ka ʻoihana maʻamau 10-32 threaded HPLC compression fittings for inlet and outlet, a ua uhi i nā palena polū o ke awa mixer kūloko.ʻO nā ʻāpana keʻa like ʻole o nā ala kahe i loko a me nā hoʻololi ʻana i ka ʻaoʻao kahe i loko o ka leo kahe o loko e hana i nā ʻāpana o ka hoʻoheheʻe ʻana a me ka laminar, e hui pū ana ma nā unahi micro, meso a me macro.Ua hoʻohana ka hoʻolālā ʻana o kēia hui hui ʻokoʻa i nā simulation computational fluid dynamics (CFD) e kālailai i nā ʻano kahe a hoʻomaʻemaʻe i ka hoʻolālā ma mua o ka hana ʻana no ka hoʻāʻo ʻana i loko o ka hale a me ka loiloi kahua kūʻai.ʻO ka hana hoʻohui ke kaʻina o ka paʻi ʻana i nā ʻāpana geometric 3D mai nā kiʻi CAD me ka ʻole o ka pono o ka mīkini kuʻuna (mīkini milling, lathes, etc.).Hoʻolālā ʻia kēia mau huila static hou e hana ʻia me ka hoʻohana ʻana i kēia kaʻina hana, kahi i hana ʻia ai ke kino mixer mai nā kaha kiʻi CAD a ua hana ʻia nā ʻāpana (paʻi ʻia) papa ma ka papa me ka hoʻohana ʻana i nā mea hoʻohui.Ma ʻaneʻi, waiho ʻia kahi ʻāpana o ka pauka metala ma kahi o 20 microns mānoanoa, a hoʻoheheʻe ʻia kahi laser i hoʻokele ʻia e ka kamepiula a hoʻoheheʻe i ka pauda i kahi ʻano paʻa.E hoʻopili i kahi papa ʻē aʻe ma luna o kēia papa a kau i ka sintering laser.E hana hou i kēia hana a hiki i ka pau ʻana o ka ʻāpana.Wehe ʻia ka pauka mai ka ʻāpana i hoʻopaʻa ʻole ʻia i ka laser, e waiho ana i kahi ʻāpana paʻi 3D e kūlike me ke kiʻi CAD kumu.ʻO ka huahana hope loa e like me ke kaʻina microfluidic, me ka ʻokoʻa nui ʻo nā ʻāpana microfluidic maʻamau ʻelua-dimensional (flat), ʻoiai me ka hoʻohana ʻana i nā mea hana hoʻohui, hiki ke hana ʻia nā ʻano kahe paʻakikī i loko o ka geometry ʻekolu-dimensional.Loaʻa kēia mau faucets e like me nā ʻāpana paʻi 3D ma 316L stainless steel a me titanium.Hiki ke hoʻohana ʻia ka hapa nui o nā ʻāpana metala, polymers a me kekahi mau seramika e hana i nā ʻāpana me kēia ʻano hana a e noʻonoʻo ʻia i nā hoʻolālā/huahana e hiki mai ana.
Laiki.1. Paʻi kiʻi (a) a me ke kiʻikuhi (b) o 90 μl Mott static mixer e hōʻike ana i kahi ʻāpana keʻa o ke ala kahe wai hui pū i uhi ʻia me ka uliuli.
E holo i ka hoʻohālikelike ʻana i ka wai hoʻohālikelike (CFD) o ka hana hui ʻana i ka wā o ka hoʻolālā ʻana e kōkua i ka hoʻomohala ʻana i nā hoʻolālā maikaʻi a hōʻemi i nā hoʻokolohua hoʻāʻo a me ka hewa.CFD simulation o static mixers a me ka paipu maʻamau (no-mixer simulation) me ka hoʻohana ʻana i ka polokalamu lako polokalamu COMSOL Multiphysics.Hoʻohālike me ka hoʻohana ʻana i nā mīkini wai laminar i hoʻoikaika ʻia e hoʻomaopopo i ka wikiwiki o ka wai a me ke kaomi i loko o kahi ʻāpana.ʻO kēia ʻano hoʻoikaika wai, i hui pū ʻia me ka lawe ʻana i nā kemika o nā pūhui ʻano paʻa, kōkua i ka hoʻomaopopo ʻana i ka hui ʻana o ʻelua mau wai ʻokoʻa.Ua aʻo ʻia ke kumu hoʻohālike ma ke ʻano he hana o ka manawa, e like me 10 kekona, no ka maʻalahi o ka helu ʻana i ka ʻimi ʻana i nā hoʻonā like.Ua loaʻa ka ʻikepili kumu i loko o kahi haʻawina i hoʻopili ʻia i ka manawa me ka hoʻohana ʻana i ka hāmeʻa projection point probe, kahi i koho ʻia ai kahi kiko ma waena o ka puka no ka hōʻiliʻili ʻikepili.Ua hoʻohana ke kŘkohu CFD a me nā ho'āʻo hoʻokolohua ʻelua mau mea hoʻoheheʻe ʻokoʻa ma o ka valve sampling proportional a me ka ʻōnaehana pumping, e loaʻa ana i kahi plug pani no kēlā me kēia solvent i ka laina sampling.Hoʻohui ʻia kēia mau mea hoʻoheheʻe i loko o kahi huila static.Hōʻike nā kiʻi 2 a me 3 i nā hoʻohālikelike kahe ma o ka paipu maʻamau (ʻaʻohe hui) a ma o ka Mott static mixer.Ua holo ka simulation ma luna o ka paipu pololei 5 knm ka lōʻihi a me ka 0.25 mm ID e hōʻike i ka manaʻo o ka alternating plugs o ka wai a me ka acetonitrile maʻemaʻe i loko o ka paipu me ka loaʻa ʻole o kahi mixer static, e like me ka mea i hōʻike ʻia ma ka Figure 2. Ua hoʻohana ka simulation i nā ana kikoʻī o ka paipu a me ka mixer a me ke kahe kahe o 0 .3 ml / min.
Laiki.2. ʻO ka hoʻohālikelike ʻana o ke kahe CFD i loko o ka paipu 5 knm me ke anawaena o loko o 0.25 mm e hōʻike i ka mea i hana ʻia i loko o kahi paipu HPLC, ʻo ia hoʻi me ka loaʻa ʻole o kahi hui.Hōʻike ka ʻulaʻula piha i ka hakina nui o ka wai.Hōʻike ka polū i ka nele o ka wai, ʻo ia hoʻi ka acetonitrile maʻemaʻe.Hiki ke ʻike ʻia nā ʻāpana hoʻoheheʻe ma waena o nā pahu ʻokoʻa o nā wai like ʻole.
Laiki.3. Mea hoʻoheheʻe static me ka nui o 30 ml, i hoʻohālikelike ʻia i ka pūʻolo polokalamu COMSOL CFD.Hōʻike ka moʻolelo i ka hapa nui o ka wai i loko o ka mea hui.Hōʻike ʻia ka wai maʻemaʻe i ka ʻulaʻula a me ka acetonitrile maʻemaʻe i ka uliuli.Hōʻike ʻia ka hoʻololi ʻana i ka hakina nui o ka wai i hoʻohālikelike ʻia e ka hoʻololi ʻana i ke kala o ka hui ʻana o nā wai ʻelua.
Ma ka fig.Hōʻike ka 4 i kahi haʻawina hōʻoia o ke kumu hoʻohālikelike ma waena o ka hoʻohui ʻana i ka maikaʻi a me ka hui pū ʻana.Ke piʻi aʻe ka nui o ka hui ʻana, e hoʻonui ka maikaʻi o ka hui ʻana.I ka ʻike o nā mea kākau, ʻaʻole hiki ke helu ʻia nā mana kino paʻakikī ʻē aʻe e hana ana i loko o ka mixer ma kēia kumu hoʻohālike CFD, e hopena i ka maikaʻi o ka hui ʻana i nā hoʻokolohua hoʻokolohua.ʻO ka hoʻokolohua hoʻohuihui ʻana ua ana ʻia e like me ka pākēneka hoʻemi i ka sinusoid kumu.Eia kekahi, ʻoi aku ka nui o ka hoʻohaʻahaʻa hope i ka hopena i nā pae huikau kiʻekiʻe, ʻaʻole i mālama ʻia i ka simulation.
Ua hoʻohana ʻia nā kūlana HPLC a me ka hoʻonohonoho hoʻāʻo ʻana e ana i nā nalu sine maka e hoʻohālikelike i ka hana pili o nā mixer static like ʻole.Hōʻike ke kiʻikuhi ma ke Kiʻi 5 i kahi hoʻolālā ʻōnaehana maʻamau HPLC/UHPLC.Ua hoʻāʻo ʻia ka mixer static ma ke kau ʻana i ka mixer ma hope o ka pauma a ma mua o ka injector a me ke kolamu hoʻokaʻawale.Hana ʻia ka hapa nui o nā ana sinusoidal hope ma ke kaʻe ʻana i ka injector a me ke kolamu capillary ma waena o ka mea hui pū me ka mea ʻike UV.I ka loiloi ʻana i ka ratio hōʻailona-a-noise a/a i ʻole ke kālailai ʻana i ke ʻano kiʻekiʻe, hōʻike ʻia ka hoʻonohonoho ʻōnaehana ma ke Kiʻi 5.
Kiʻi 4. ʻO ka ʻāpana o ka hoʻohui ʻana i ka maikaʻi me ka hoʻohui ʻana i ka leo no ka laulima o nā mea hui pū.ʻO ka haumia theoretical e hahai ana i ke ʻano like me ka ʻikepili haumia hoʻokolohua e hōʻoia ana i ka pono o nā simulation CFD.
ʻO ka ʻōnaehana HPLC i hoʻohana ʻia no kēia hoʻāʻo he Agilent 1100 Series HPLC me kahi mea ʻike UV i hoʻomalu ʻia e kahi PC e holo ana i ka polokalamu Chemstation.Hōʻike ka Papa 1 i nā kūlana hoʻolohe maʻamau no ke ana ʻana i ka pono o ka mixer ma o ka nānā ʻana i nā sinusoid kumu i loko o ʻelua mau hihia.Ua hana ʻia nā hoʻokolohua hoʻokolohua ma nā laʻana like ʻole ʻelua o nā solvents.ʻO nā mea hoʻoheheʻe ʻelua i hui pū ʻia i ka hihia 1 he solvent A (20 mM ammonium acetate i ka wai deionized) a me ka solvent B (80% acetonitrile (ACN)/20% wai deionized).Ma ka hihia 2, ʻo ka solvent A he hopena o 0.05% acetone (label) i loko o ka wai deionized.He hui ʻia ʻo Solvent B me 80/20% methanol a me ka wai.Ma ka hihia 1, ua hoʻonohonohoʻia ka paila i ka holoʻana o ka 0.25 ml / min i ka 1.0 ml / min, a i ka hihia 2, ua hoʻonohonohoʻia ka paila i kahi kahe mau o 1 ml / min.I nā hihia ʻelua, ʻo ka lakio o ka hui ʻana o nā solvents A a me B he 20% A / 80% B. Ua hoʻonohonoho ʻia ka mea ʻike i ka 220 nm i ka hihia 1, a ʻo ka nui o ka absorption o ka acetone i ka hihia 2 i hoʻonohonoho ʻia i ka nalu o 265 nm.
Papa 1. Hoʻonohonoho HPLC no nā hihia 1 a me 2 hihia 1 Case 2 Pump Speed ​​​​0.25 ml/min a hiki i 1.0 ml/min 1.0 ml/min Solvent A 20 mM ammonium acetate i ka wai deionized 0.05% Acetone i ka wai deionized Solvent B 80% Acetonitrile / 20% deionized wai (ACN% deionized) wai Lakiona solvent 20% A / 80% B 20% A / 80% B Mea ʻike 220 nm 265 nm
Laiki.6. ʻO nā ʻāpana o nā hawewe sine huikau i ana ʻia ma mua a ma hope o ka hoʻohana ʻana i kahi kānana ala haʻahaʻa e wehe i nā ʻāpana neʻe kumu o ka hōʻailona.
He la'ana ma'amau ka Figure 6 o ka leo ha'aha'a huikau ma ka hihia 1, i hō'ike 'ia ma ke 'ano he kumu sinusoidal hou i ho'opa'a 'ia ma luna o ka ne'e kumu.ʻO ka hoʻoheheʻe ʻana o ka papa kuhikuhi he piʻi lohi a hoʻemi paha i ka hōʻailona hope.Inā ʻaʻole ʻae ʻia ka ʻōnaehana e hoʻohālikelike i ka lōʻihi, e hāʻule maʻamau ia, akā e neʻe hewa ʻole ʻoiai inā paʻa loa ka ʻōnaehana.Ke piʻi aʻe nei ka neʻe ʻana o ka baseline i ka wā e hana ana ka ʻōnaehana i ka gradient kiʻekiʻe a i ʻole nā ​​kūlana kiʻekiʻe o ke kua.I ka wā e hiki mai ana kēia drift baseline, hiki ke paʻakikī ke hoʻohālikelike i nā hualoaʻa mai ka laʻana i ka laʻana, hiki ke lanakila ma ka hoʻohana ʻana i kahi kānana haʻahaʻa i ka ʻikepili maka e kānana i kēia mau ʻokoʻa haʻahaʻa haʻahaʻa, a laila e hāʻawi i kahi ʻāpana oscillation me kahi papa kuhikuhi.Ma ka fig.Hōʻike pū ka Figure 6 i kahi ʻāpana o ka walaʻau kumu o ka mea hui ma hope o ka hoʻohana ʻana i kahi kānana haʻahaʻa.
Ma hope o ka hoʻopau ʻana i nā hoʻohālikelike CFD a me ka hoʻāʻo hoʻokolohua mua, ua hoʻomohala ʻia ʻekolu mau huila static kaʻawale me ka hoʻohana ʻana i nā ʻāpana kūloko i wehewehe ʻia ma luna me ʻekolu mau puke i loko: 30 µl, 60 µl a me 90 µl.Hoʻopili kēia pae i ka laulā o nā puke a me ka hoʻohui ʻana i nā hana e pono ai no nā noi HPLC analyte haʻahaʻa kahi e pono ai ka hui ʻana a me ka hoʻopuehu haʻahaʻa e hana i nā baseline amplitude haʻahaʻa.Ma ka fig.Hōʻike ʻo 7 i nā ana hawewe sine maʻamau i loaʻa ma ka ʻōnaehana hoʻāʻo o ka laʻana 1 (acetonitrile a me ammonium acetate ma ke ʻano he tracers) me ʻekolu mau leo ​​o nā mea huila static a ʻaʻohe mea hui.ʻO nā kūlana hoʻokolohua hoʻokolohua no nā hopena i hōʻike ʻia ma ke Kiʻi 7 ua paʻa mau i nā hoʻokolohua 4 āpau e like me ke kaʻina hana i hōʻike ʻia ma ka Papa 1 ma kahi kahe kahe o ka solvent o 0.5 ml/min.E hoʻopili i kahi waiwai offset i nā ʻikepili i hiki ke hōʻike ʻia i kēlā me kēia ʻaoʻao me ka ʻole o ke kau ʻana o ka hōʻailona.ʻAʻole pili ka Offset i ka amplitude o ka hōʻailona i hoʻohana ʻia e hoʻoponopono i ka pae hana o ka mea hui.ʻO ka amplitude sinusoidal awelika me ka mea hui ʻole he 0.221 mAi, ʻoiai ua hāʻule nā ​​amplitudes o nā hui Mott static ma 30 µl, 60 µl, a me 90 µl i 0.077, 0.017, a me 0.004 mAi.
Kiʻi 7. HPLC UV Detector Signal Offset vs. Time for Case 1 (acetonitrile with ammonium acetate indicator) e hōʻike ana i ka hui ʻana o ka solvent me ka hui ʻole ʻana, 30 µl, 60 µl a me 90 µl Mott mixers e hōʻike ana i ka hoʻohui maikaʻi ʻana (haʻahaʻa hōʻailona amplitude) e like me ka piʻi ʻana o ka nui o ka static.(nā ʻikepili maoli: 0.13 (ʻaʻohe hui pū), 0.32, 0.4, 0.45mA no ka hōʻike maikaʻi ʻana.
ʻO ka ʻikepili i hōʻike ʻia ma ka fig.Ua like ka 8 me ka Fig. 7, akā i kēia manawa ua hoʻokomo lākou i nā hopena o ʻekolu mea hoʻohana maʻamau HPLC static mixers me nā puke o loko o 50 µl, 150 µl a me 250 µl.Laiki.Kiʻi 8. HPLC UV Detector Signal Offset versus Time Plot no ka hihia 1 (Acetonitrile a me Ammonium Acetate ma ke ʻano he mau hōʻailona) e hōʻike ana i ka hui ʻana o ka solvent me ka ʻole static mixer, ka moʻo hou o Mott static mixers, a me ʻekolu mixers maʻamau (ʻike maoli offset ʻo 0.1 (me ka hui ʻole ʻole), 0.48, 0.9, 0.48, 0.9 ʻO kēlā me kēia no ka hopena hōʻike maikaʻi aʻe).Ua helu ʻia ka pākēneka o ka hawewe sine kumu e ka ratio o ka amplitude o ka hawewe sine i ka amplitude me ka hoʻokomo ʻole ʻia o ka mixer.Hoʻopaʻa ʻia nā pākēneka atenuation o ka hawewe sine no nā hihia 1 a me 2 ma ka Papa 2, me nā puke o loko o kahi mixer static hou a me ʻehiku mea hui maʻamau i hoʻohana mau ʻia i ka ʻoihana.Hōʻike nā ʻikepili i nā Kiʻi 8 a me 9, a me nā helu helu i hōʻike ʻia ma ka Papa 2, hiki i ka Mott Static Mixer ke hāʻawi i ka 98.1% attenuation sine wave, ʻoi aku ma mua o ka hana o kahi huila HPLC maʻamau ma lalo o kēia mau kūlana hoʻāʻo.Kiʻi 9. HPLC UV detector hōʻailona offset versus time plot no case 2 (methanol and acetone as tracers) e hōʻike ana ʻaʻohe static mixer (hui pū ʻia), he moʻo hou o Mott static mixers a me ʻelua mixers maʻamau (ʻo 0, 11 (me ka hui ʻole. ), 0.22 a me 0.35, mA maikaʻi.Ua loiloi pū ʻia nā mea huila ʻehiku i hoʻohana mau ʻia i ka ʻoihana.Hoʻopili kēia i nā mea hui pū me ʻekolu mau puʻupuʻu kūloko ʻokoʻa mai ka hui A (i koho ʻia ʻo Mixer A1, A2 a me A3) a me ka hui B (i koho ʻia ʻo Mixer B1, B2 a me B3).Hoʻokahi wale nō ka nui o ka hui C.
Papa 2. Nā ʻano o ka hoʻoulu ʻana o ka Mixer Static a me ka Volume Internal Static Mixer Case 1 Sinusoidal Recovery: Acetonitrile Test (Efficiency) Case 2 Sinusoidal Recovery: Methanol Water Test (Efficiency) Internal Volume (µl) No Mixer – - 0 Mott 30 65% 67.2% 30 Mott 67.2% 30 Mott 67.2% 30 Mott 67.2% 30 Mott 67.2% %. 4% 250
Hōʻike ka ʻike ʻana o nā hopena ma ke Kiʻi 8 a me ka Papa 2, ʻo ka 30 µl Mott static mixer ua like ka maikaʻi o ka hui ʻana me ka hui A1, ʻo ia hoʻi 50 µl, akā naʻe, ʻo ka 30 µl Mott he 30% emi iho ka nui o loko.I ka hoʻohālikelike ʻana i ka 60 µl Mott mixer me ka 150 µl internal volume A2 mixer, aia kahi hoʻomaikaʻi iki i ka hoʻohui ʻana i ka pono o 92% versus 89%, akā ʻoi aku ka mea nui, ua loaʻa kēia pae kiʻekiʻe o ka hui ʻana ma ka 1/3 o ka nui mixer.mea hui like A2.ʻO ka hana o ka 90 µl Mott mixer i hahai i ke ʻano like me ka A3 mixer me ka nui o loko o 250 µl.ʻO ka hoʻomaikaʻi ʻana i ka hoʻohui ʻana i ka hana o 98% a me 92% i ʻike pū ʻia me ka hōʻemi ʻana o 3-fold i ka nui o loko.Ua loaʻa nā hopena like a me nā hoʻohālikelike no nā mixers B a me C. ʻO ka hopena, ʻo ka moʻo hou o nā mixers static Mott PerfectPeakTM e hāʻawi i ka ʻoi aku ka maikaʻi o ka hui ʻana ma mua o nā mea hoʻokūkū hoʻokūkū hoʻohālikelike, akā me ka liʻiliʻi o loko, e hāʻawi ana i ka leo ʻoi aku ka maikaʻi a me ka ʻoi aku ka maikaʻi o ka hōʻailona-i-noise ratio, ʻoi aku ka maikaʻi o ka Analyte, ke ʻano kiʻekiʻe a me ka hoʻonā kiʻekiʻe.Ua ʻike ʻia nā ʻano like o ka hui ʻana i ka maikaʻi ma ka hihia 1 a me ka hihia 2.No ka hihia 2, ua hana ʻia nā hoʻāʻo me ka hoʻohana ʻana i (methanol a me acetone ma ke ʻano he mau hōʻailona) e hoʻohālikelike i ka pono o ka hui ʻana o 60 ml Mott, kahi hui like A1 (50 µl kūloko) a me kahi hui hui like B1 (volume kūloko 35 µl)., maikaʻi ʻole ka hana me ka hoʻokomo ʻole ʻia o kahi huila, akā ua hoʻohana ʻia no ka loiloi kumu.ʻO ka 60 ml Mott mixer i hōʻike ʻia ʻo ia ka mea hui maikaʻi loa i ka hui hoʻāʻo, e hāʻawi ana i ka hoʻonui ʻana o 90% o ka hui ʻana.Ua ʻike ʻia kahi Mixer A1 hoʻohālikelike i ka hoʻomaikaʻi ʻana o 75% i ka maikaʻi o ka hui ʻana a ukali ʻia e ka hoʻomaikaʻi ʻana o 45% i kahi hui B1 like.Ua hana ʻia kahi hoʻāʻo hōʻemi hawewe sinus maʻamau me ka kahe kahe ʻana ma ke ʻano o nā huila ma lalo o nā kūlana like me ka hoʻāʻo ʻana i ka curve sine ma ka hihia 1, me ka hoʻololi ʻana o ka kahe kahe.Ua hōʻike ʻia ka ʻikepili ma ka laulā o nā kahe kahe mai ka 0.25 a i ka 1 ml / min, ʻo ka hoʻohaʻahaʻa mua ʻana o ka hawewe sine i mau ʻia no nā puʻupuʻu mixer ʻekolu.No nā hui hui liʻiliʻi liʻiliʻi ʻelua, aia kahi hoʻonui liʻiliʻi o ka sinusoidal contraction e like me ka emi ʻana o ke kahe kahe, ka mea i manaʻo ʻia ma muli o ka hoʻonui ʻana o ka manawa noho o ka mea hoʻoheheʻe i loko o ka mea hui, e ʻae ai i ka hoʻonui ʻana i ka hui ʻana.Manaʻo ʻia e hoʻonui ka unuhi ʻana o ka hawewe sine i ka emi ʻana o ke kahe.Eia nō naʻe, no ka leo huila nui loa me ka attenuation base hawewe kiʻekiʻe loa, ʻaʻole i hoʻololi ʻia ka attenuation base wave sinus (i loko o ka laulā o ka maopopo ʻole o ka hoʻokolohua), me nā waiwai mai 95% a 98%.Laiki.10. ʻO ka attenuation maʻamau o kahi hawewe sinus me ke kahe kahe i ka hihia 1. Ua hana ʻia ka hoʻāʻo ma lalo o nā kūlana e like me ka hoʻāʻo ʻana me ka hoʻololi ʻana o ka kahe kahe ʻana, e hoʻokomo i 80% o kahi hui 80/20 o ka acetonitrile a me ka wai a me 20% o 20 mM ammonium acetate.
ʻO ka laulā hou o nā mea hoʻohui static inline PerfectPeakTM i hoʻopaʻa ʻia me ʻekolu mau puke i loko: 30 µl, 60 µl a me 90 µl uhi i ka leo a me ka hoʻohui ʻana i nā hana hana e pono ai no ka hapa nui o nā loiloi HPLC e koi ana i ka hoʻomaikaʻi ʻana i ka hui ʻana a me nā papahele haʻahaʻa haʻahaʻa.Hoʻokō ka mea huila static hou i kēia ma ka hoʻohana ʻana i ka ʻenehana paʻi 3D hou e hana i kahi ʻano 3D kūʻokoʻa e hāʻawi ana i ka hoʻohuihui static hydrodynamic i hoʻomaikaʻi ʻia me ka haʻahaʻa haʻahaʻa kiʻekiʻe o ka walaʻau kumu no ka nui o ka hui o loko.ʻO ka hoʻohana ʻana i ka 1/3 o ka leo kūloko o kahi hui hui maʻamau e hōʻemi i ka leo kumu ma 98%.ʻO ia mau mea hui pū ʻia nā kahawai kahe ʻekolu-dimensional me nā ʻāpana keʻa like ʻole a me nā lōʻihi ala like ʻole i ka wā e hele ai ka wai i nā pale geometric paʻakikī i loko.Hāʻawi ka ʻohana hou o nā mixer static i ka hana i hoʻomaikaʻi ʻia ma luna o nā mea hoʻokūkū hoʻokūkū, akā me ka liʻiliʻi o ka leo kūloko, ka hopena i ka ratio hōʻailona-i-noise maikaʻi a me nā palena quantitation haʻahaʻa, a me ka hoʻomaikaʻi ʻana i ke ʻano o ka piko, ka pono a me ka hoʻonā no ka ʻike kiʻekiʻe.
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