“Tsis txhob ua xyem xyav tias ib pawg me me ntawm cov neeg xav txog, cov pej xeem mob siab rau tuaj yeem hloov pauv lub ntiaj teb.Qhov tseeb, nws tsuas yog ib qho nyob ntawd. "
Cureus 'lub hom phiaj yog los hloov tus qauv ntev ntawm kev tshaj tawm kho mob, uas qhov kev tshawb fawb xa tuaj yuav kim, nyuaj, thiab siv sijhawm.
Platelet-nplua nuj plasma / prp, cov ntaub so ntswg regeneration, platelet activation, glucose proliferative therapy, platelets, proliferative therapy
Sau cov kab lus no xws li: Harrison TE, Bowler J, Reeves K, thiab al.(Tsib Hlis 17, 2022) Cov txiaj ntsig ntawm cov piam thaj ntawm platelet suav thiab ntim: cuam tshuam rau cov tshuaj kho dua tshiab.Kho 14(5): e25081.doi: 10.7759/cureus.25081
Platelet-nplua nuj plasma (PRP) thiab cov tshuaj hypertonic qabzib feem ntau yog siv rau kev txhaj tshuaj hauv cov tshuaj regenerative, qee zaum ua ke.Cov nyhuv ntawm hypertonic qabzib ntawm platelet lysis thiab kev ua kom tsis tau raug tshaj tawm yav dhau los.Peb tau soj ntsuam cov txiaj ntsig ntawm cov piam thaj ntau ntxiv ntawm platelet thiab erythrocyte suav, nrog rau cov cell ntim hauv PRP thiab tag nrho cov ntshav (WB).Kev txo qis ib feem ntawm platelet suav tau tshwm sim nrog tag nrho cov piam thaj sib xyaw nrog PRP lossis tag nrho cov ntshav, raws li ib feem ntawm lysis. Tom qab thawj feeb, platelet suav tseem nyob ruaj khov, qhia txog kev ceev ceev ntawm cov platelets seem mus rau qhov siab (> 2000 mOsm) hypertonicity. Tom qab thawj feeb, platelet suav tseem nyob ruaj khov, qhia txog kev ceev ceev ntawm cov platelets seem mus rau qhov siab (> 2000 mOsm) hypertonicity. После первой минуты количество тромбоцитов оставалось стабильным, что указывает на быструов аккоютрода до экстремального (> 2000 мОсм) гипертонуса. Tom qab thawj feeb, cov platelet suav tseem nyob ruaj khov, qhia tau tias muaj kev pabcuam sai ntawm cov platelets seem mus rau qhov siab (> 2000 mOsm) hypertonicity.第一分钟后，血小板计数保持稳定，表明残余血小板迅速适应极端（> 2000 mOsm 灶端）.2000 mOsm) 高渗状态. После первой минуты количество тромбоцитов оставалось стабильным, что указывает на быструох адапртациты экстремальному (> 2000 мосм) гиперосмолярному состоянию. Tom qab thawj feeb, cov platelet suav tseem nyob ruaj khov, qhia txog kev hloov pauv sai ntawm cov platelets seem mus rau qhov siab (> 2000 mOsm) hyperosmolar xeev.Glucose concentrations ntawm 25% thiab saum toj no ua rau muaj kev nce ntxiv hauv cov ntsiab lus platelet ntim (MPV), qhia txog theem pib ntawm platelet activation.Cov kev tshawb fawb ntxiv yuav tsum tau txiav txim siab seb platelet lysis lossis kev ua kom tshwm sim thiab seb puas yog kev txhaj tshuaj hypertonic glucose ib leeg lossis ua ke nrog PRP tuaj yeem muab cov txiaj ntsig kho mob ntxiv.
Nyob rau xyoo 1950, tus kws phais neeg Asmeskas George Hackett pom tias nws tuaj yeem txo qhov mob thiab rov qab mus tas li hauv ntau tus neeg mob los ntawm kev txhaj tshuaj tiv thaiv kab mob rau hauv cov leeg thiab ligaments.Nws qhov kev sim ntawm cov luav tau pom tias qhov kev kho mob, uas nws hu ua kev kho mob proliferative, ua rau cov leeg kom loj thiab ntxiv dag zog.Cov kev tshawb fawb histological tau lees paub tias cov collagen tshiab yog tsim los ntawm cov txheej txheem no [1].
Hauv thawj ob peb lub xyoo caum, ntau qhov kev faib tawm sib txawv tau sim.Los ntawm xyoo 1990, cov kws kho mob feem ntau suav tias muaj cov piam thaj ntau ntau los ua txoj hauv kev nyab xeeb thiab zoo tshaj plaws.Txawm li cas los xij, lub mechanism ntawm kev txiav txim tseem tsis meej.
Qee qhov kev tshawb fawb soj ntsuam tau ua nyob rau xyoo pua 20th tom qab Hackett txoj haujlwm.Txawm li cas los xij, nyob rau xyoo 2000s muaj kev txaus siab tshiab thiab ntau qhov kev sim ua tiav ntawm kev kho mob proliferative tau ua tiav rau kev kho mob qis rov qab [2], osteoarthritis ntawm lub hauv caug [3], thiab lateral epicondylitis [4].
Cov ntaub so ntswg regeneration yuav tsum muaj kev koom tes ntawm qia hlwb.Yog li ntawd, qhov siab ntawm cov piam thaj yuav tsum ua kom muaj kev tsiv teb tsaws, rov ua dua, thiab sib txawv ntawm cov qia hlwb.Peb xav tias platelets tuaj yeem ua raws li cov neeg xa xov thiab cov piam thaj ntau yuav ua rau platelets tso tawm cytokines thiab kev loj hlob yam, yog li txhawb cov txheej txheem rov tsim dua tshiab, tshwj xeeb tshaj yog qia cell tsiv teb tsaws mus rau thaj chaw ntawm cov piam thaj siab.
Platelet activation ib txwm ua ntej qhov nce hauv cov calcium hauv lub cev [5].Liu et al.Hauv xyoo 2008 tau pom tias cov piam thaj ntau ntxiv ua rau muaj kev ua haujlwm ntawm cov neeg hloov pauv hloov pauv muaj peev xwm canonical hom 6 (TRPC6) raws hauv cov ntshav plasma, uas ua rau muaj cov calcium ions nkag mus rau hauv platelets [6].Lwm txoj kev tshawb fawb pom tau hais tias raug cov microtubule marginal cheeb tsam rau calcium ions ua rau so, nthuav, thiab deformation ntawm marginal cheeb tsam, uas nyob rau hauv lem ua rau ib tug hloov nyob rau hauv cov duab los ntawm disc mus rau spherical, uas ua rau lub platelet volume (MPV) [7].
Peb qhov kev xav hauv qhov kev tshawb fawb no yog tias qhov tshwm sim ntawm platelets rau cov piam thaj ntau ntau cuam tshuam rau thaj tsam microtubule marginal thiab intracellular ib puag ncig, ua rau muaj kev nce hauv MPV.
Txhua tus neeg koom nrog kos npe rau daim ntawv tso cai pom zoo tom qab tau piav qhia cov ntsiab lus ntawm qhov kev kawm thiab ua ntej tau txais cov qauv.Hauv txoj kev tshawb no, tsuas yog cov qauv PRP uas muaj hematocrit ntau dua 2% tau siv kom erythrocyte (erythrocyte) suav thiab txhais tau tias lub cev ntim ntawm cov qe ntshav liab (MCV) tuaj yeem suav nrog rau kev sib piv.
Txoj kev tshawb no tau ua nyob rau hauv plaub theem, thawj theem yog PRP thiab cov theem ntxiv yog cov ntshav tag nrho (Table 1).Raws li tau piav qhia yav dhau los [8], tag nrho cov txheeb ze centrifugal rog (RCF, g-force) raug xam los ntawm qhov nruab nrab (Rmid, hauv cm) ntawm cov ntshav kem hauv centrifugal syringe.Peb tau xaiv los siv MPV ua tus cim ntawm platelet rhiab heev thiab platelet suav raws li qhov taw qhia ntawm qhov muaj peev xwm platelet lysis, ob qho tib si tuaj yeem ntsuas tau yooj yim ntawm tus qauv hematology analyzers.
Hauv thawj theem, 47 tus neeg tuaj yeem pub dawb kuaj ntshav - ib lub raj ntawm ethylenediaminetetraacetic acid (EDTA) thiab ib qho PRP tag nrho cov ntshav kuaj (anticoagulated nrog sodium citrate (NaCl, 3%)) (Table 1).Muab lub rocker tso rau hauv lub raj tam sim ntawd.Ua tiav cov ntshav suav (CBC) tau ua tiav ntawm EDTA cov qauv hauv triplicate, thiab NaCl cov qauv tau txheeb xyuas hauv triplicate rau CBC tsom xam, thiab tom qab ntawd PRP tau npaj los ntawm ntau txoj hauv kev tau piav qhia saum toj no [8].Tag nrho cov qauv PRP tau npaj los ntawm centrifugation ntawm 900-1000 g.Sib tov txhua tus qauv PRP ntawm lub tshuab vortex rau 5-10 vib nas this, tom qab ntawd faib tsib 0.5 ml aliquots rau hauv cov raj.
Txhawm rau ntsuas cov nyhuv ntawm cov platelet sib kis rau cov ntshav siab, muaj li ntawm 0%, 5,5%, 5,5%, 5,5%, thiab 25%, thiab 25% thiab 25% thiab 25%TAC ntawm txhua qhov sib tov tau txheeb xyuas hauv triplicate tom qab 15 feeb.Platelet suav (PLT), RBC suav, MCV, thiab MPV tau nruab nrab rau txhua lub raj, thiab txhais tau tias platelet suav, RBC suav, MCV, thiab MPV raug xam rau tag nrho cov qauv PRP.
Tom qab thawj theem ntawm kev sau cov ntaub ntawv tiav, peb pom muaj qhov nce ntxiv ntawm platelet ntim hauv PRP platelets tom qab ntxiv ntawm D50W.PRP platelets tsis tas sawv cev rau tag nrho cov platelets hauv cov ntshav, thiab PRP nruab nrab txawv ntawm WB nruab nrab.Yog li ntawd, peb txiav txim siab los ua qhov kev sim thib ob ntawm cov txiaj ntsig ntawm kev ntxiv D50W rau tag nrho cov ntshav.
Rau qhov thib ob, peb xaiv ib qho piv txwv loj ntawm 30 raws li cov txiaj ntsig ntawm thawj koob, raws li tau piav qhia hauv ntu Kev Tshawb Fawb.Hauv cov koob no, 20 tus neeg tuaj yeem pab dawb pub ntshav kuaj (Table 1).Tag nrho cov ntshav (1.8 ml) tau kos rau hauv 3 ml syringe thiab anticoagulated nrog 0.2 ml 40% NaCl.Tag nrho cov ntshav syringe tau tov rau tsib vib nas this nrog lub vortex tov khoom thiab CBC tau soj ntsuam hauv triplicate.Tom qab kev soj ntsuam, cov ntshav anticoagulated tau ntxiv rau 2 ml ntawm 50% qabzib hauv 5 ml syringe (qhov kawg cov piam thaj concentration yog kwv yees li 25% (D25)) thiab muab tso rau hauv lub raj co rau 30 feeb. Tom qab 30 feeb, D25 / CBC hauv WB syringes tau txheeb xyuas hauv triplicate. Platelet suav, MCV, RBC thiab suav nruab nrab. suav, MCV, thiab MPV raug xam rau txhua tus qauv ua ntej thiab tom qab ntxiv cov piam thaj.
Vim tias platelets nyob rau hauv tag nrho cov ntshav feem ntau raug rau hypertonic qabzib thaum lub sij hawm proliferative qabzib txoj kev kho mob vim qhov tsawg invasive txhaj, thiab nws tsis yog ib txwm ua ke PRP nrog hypertonic qabzib ua ntej txhaj tshuaj, peb txiav txim siab los kawm hypertonic qabzib ua ke nrog WB nyob rau hauv Tshooj 1. Kauj Ruam Peb thiab plaub.Hauv txhua theem, 20 tus neeg tuaj yeem pub dawb pub dawb 7-8 ml ntawm ACD-A (acid muaj trisodium citrate (22.0 g / l), citric acid (8.0 g / l) thiab qabzib (24.5 g / l), tshuaj dextrose citrate) rau cov ntshav anticoagulants (Table 1).Tsuas yog cov khoom sib xyaw ntawm cov piam thaj ntau dua 12.5% ​​​​yog siv los txiav txim qhov feem pua ​​​​uas cuam tshuam nrog kev nce hauv MPV.Nyob rau theem peb, 1 ml ntawm cov ntshav yog muab tso rau hauv ib lub raj kuaj.Tom qab ntawd sib tov cov ntshav ntawm vortex tov rau 10 vib nas this los ntawm kev ntxiv 1 ml ntawm 30% qabzib, 40% qabzib, lossis 50% qabzib mus rau lub raj kom tau txais cov piam thaj kawg ntawm 15%, 20%, thiab 25%, feem.Kev kuaj ntshav qabzib tau raug tshuaj xyuas rau CBC tam sim ntawd tom qab sib xyaw thiab rov ua dua txhua ob feeb rau 30 feeb.
Thaum pib sib xyaw, qhov sib ntxiv ntawm 1: 1 hypertonic qabzib thiab WB lossis PRP nthuav tawm cov platelets kom siab tshaj 25% rau ob peb feeb.Hauv qib plaub, txhawm rau ntsuas cov txiaj ntsig ntawm hypertonic qabzib nrog qhov tsawg kawg nkaus qhov siab tshaj plaws thiab ntsuas qhov siab tshaj ntawm cov nyhuv ntawm cov piam thaj, peb ntxiv cov ntshav me me rau D25W lossis D50W.Muab 1 ml ntawm D25W los yog D50W rau hauv ib lub raj thiab ntxiv 0.2 ml ntawm WB thaum vortexing tus qauv rau 10 vib nas this.Hauv cov xwm txheej no, cov ntshav tau raug cov piam thaj ntawm qhov concentration kwv yees li 20% saum toj kawg nkaus concentration, ntau dua li 50% siab dua qhov kawg siab kawg nkaus li nyob rau theem 3, ua rau cov piam thaj kawg ntawm 20.8% thiab 41.6%.Cov qauv sib xyaw tau txheeb xyuas tib lub sijhawm raws li hauv qib 3.
Hauv thawj kauj ruam ntawm txhua cov piam thaj dilution series, 30 cov qauv raug coj mus kuaj vim qhov no yog qhov qauv tsim nyog rau kev sim ntsuas [9].Thaum kawg ntawm txhua theem (nrog rau thawj theem), ntsuas qhov txaus ntawm tus qauv loj uas siv cov qauv siv los txiav txim seb tus qauv loj xav tau los kwv yees qhov txiaj ntsig ntawm qhov txiaj ntsig tsis tu ncua ntawm ib tus neeg.Formula n = Z2 x SD2 /E2.Hauv qhov sib npaug no, Z yog tus qhab-nees Z, SD yog tus qauv sib txawv, thiab E yog qhov xav tau yuam kev [10].Peb alpha yog 0.05, uas sib haum rau Z tus nqi ntawm 1.96, thiab peb cia siab tias qhov yuam kev ntawm 5 (hauv feem pua).Li no peb daws rau n = (1.962 x SD2)/52.Cov txiaj ntsig tau pom tias tus qauv loj uas yuav tsum tau muaj rau txhua theem yog me dua li tus lej tiag.
Thaum lub sij hawm 1, 3 thiab 4 siv ntau dua ib qho qabzib concentration, cov txiaj ntsig ntawm cov piam thaj sib txawv tau txheeb xyuas los ntawm kev sib piv cov feem hloov pauv ntawm lub sijhawm 0 thiab txhua lub sijhawm tom ntej (theem 1 ntawm 15 feeb, lub sijhawm 3 ntawm 15 feeb).thiab plaub ntawm 15 vib nas this, tom qab ntawd txhua ob feeb.) Cov nqi hloov pauv rau txhua lub sijhawm tau muab piv nrog kev siv Mann-Whitney U-test vim tias cov ntaub ntawv tsis ua raws li ib txwm faib raws li tau txiav txim los ntawm Shapiro-Wilk normality test.Txij li thaum 1-rau-1 kev tshuaj xyuas ntawm ntau pawg (tsib) tau ua nyob rau hauv thawj, thib peb thiab thib plaub theem (tsib nyob rau hauv tag nrho), kev kho Bonferroni tau ua los kho qhov xav tau alpha tus nqi rau ≤0.01 tab sis tsis yog ≤0.05.
Txo cov platelet suav nrog tag nrho cov concentration ntawm hypertonic dextrose thiab kev nce hauv MPV hauv PRP platelets ntawm> 12.5% ​​dextrose concentration: PRP platelet suav tau nce los ntawm ib mus rau tsib zaug concentration piv rau cov ntshav hauv qab, sib txawv los ntawm txoj kev (tsis yog piav qhia). Txo cov platelet suav nrog tag nrho cov concentration ntawm hypertonic dextrose thiab nce hauv MPV hauv PRP platelets ntawm> 12.5% ​​dextrose concentration: PRP platelet suav tau nce los ntawm ib mus rau tsib zaug concentration piv rau cov ntshav hauv qab, sib txawv los ntawm txoj kev (tsis yog piav qhia). Уменьшение количества тромбоцитов при всех концентрациях гипертонической декстрозы и увеличение MPV и увеличение MPV трации декстрозы > 12,5%: количество тромбоцитов PRP увеличилось в 1-5 раз по сравнению с исходнойцей , от метода (nе показано). Txo cov platelet suav ntawm tag nrho cov hypertonic dextrose concentrations thiab nce MPV hauv PRP platelets ntawm> 12.5% ​​dextrose concentration: PRP platelet suav nce 1-5 zaug piv rau cov ntshav hauv qab, nyob ntawm txoj kev (tsis qhia). ).在> 12.5% ​​的葡萄糖浓度下，所有浓度的高渗葡萄糖降低血小板计数，PRP 血小板丞忠功比，PRP 血小板计数从浓度的1倍上升到5倍，因方法而异（未描述）。 Ntawm> 12.5% ​​​​glucose concentration, siab concentration ntawm qabzib txo cov ntshav suav, PRP ntshav MPV nce: piv rau 与基线全血, PRP ntshav suav nce los ntawm 1 txog 5 npaug ntawm cov concentration (tsis piav qhia). При концентрациях глюкозы > 12,5% все концентрации гипертонической глюкозы снижали количестовы шртонической оцитах PRP: количество тромбоцитов PRP увеличивалось от 1- до 5-кратных концентраций по сравненикь с истравненикь с иси ой крови, в зависимости от метода (не описано ). Ntawm cov piam thaj ntau> 12.5%, tag nrho cov ntshav qabzib ntau cov ntshav txo qis platelet suav thiab nce MPV hauv PRP platelets: PRP platelet suav tau nce 1- txog 5-fold piv rau cov hauv paus ntshav siab tag nrho, nyob ntawm txoj kev (raws li tau piav qhia).Daim duab 1 qhia tau hais tias cov platelets txo los ntawm yuav luag 75% tom qab dilution hauv dej thiab los ntawm 20-30% tom qab 15 feeb ntawm dilution nrog txawv concentrations ntawm qabzib piv rau hauv paus PRP thiab ib tug 1: 1 dilution kho rau ntim (1- k1 nrog ntim kho).k -1 kev yug me nyuam).1 yug me nyuam).
Tus naj npawb ntawm cov hlwb hauv txhua qhov dilution yog qhia raws li ib feem ntawm tus lej qub ua ntej dilution.
MPV txo qis thaum lub sij hawm PRP ntau lawm, yam tsis muaj kev hloov pauv ntxiv hauv dilution concentrations mus rau 12.5% ​​hauv dej lossis qabzib (xws li 25% PRP qabzib sib tov) thiab nce ntau dua 20% tom qab dilution hauv 50% qabzib tov (Fig. .2).).Hauv qhov sib piv, erythrocytes pom tsis muaj qhov hloov pauv loj hauv ntim ntawm txhua qhov dilution dua li H2O.
Qhov nruab nrab ntim ntawm cov hlwb hauv txhua qhov dilution yog qhia raws li feem pua ​​​​ntawm cov thawj ntim ua ntej dilution.
Ib qho zoo sib xws tab sis tsis tshua muaj kev txo qis hauv platelet suav thiab nce hauv CVR tau pom nyob rau hauv BC raug rau 50% qabzib (kom tsim nrog 25% qabzib).Table 2 piv cov xov tooj ntawm tes thiab cov xov tooj ntawm tes hauv tag nrho cov ntshav diluted hauv 50% dextrose nrog rau theem 1 PRP cov ntaub ntawv diluted hauv 50% dextrose.Kev hloov pauv hauv RBC suav thiab RBC MCV tsis pom tseeb thiab tsis yog qhov tseem ceeb ntawm peb cov kev xav.
SD = standard deviation, MD = txhais tau tias sib txawv ntawm pab pawg, SE = standard deviation ntawm txhais tau tias sib txawv, RBC = erythrocytes, PLT = platelets, PRP = platelet nplua nuj plasma, WB = tag nrho cov ntshav
Tom qab ntxiv D50W rau WB, qhov feem pua ​​​​ntawm cov platelet poob yog 7.7% (310 ± 73 vs. 286 ± 96) piv rau 17.8% rau PRP dilution hauv D50W (664 ± 348 vs. 544 ± 277).MPV WB nce 16.8% (los ntawm 10.1 ± 0.5 txog 11.8 ± 0.6), thaum MPV PRP nce 26% (9.2 ± 0.8 vs. 11.6 ± 0.7). Txawm hais tias qhov sib txawv ntawm ob qho tib si platelet suav txo thiab MPV nce ntxiv nrog PRP, qhov kev hloov pauv ntawm platelet suav txo hauv WB tau ze li qhov tseem ceeb (310 ± 73 txog 286 ± 96 (-7.7%)); p = .06) thiab qhov nce hauv MPV yog qhov tseem ceeb (10.1 ± 0.5 + 10.8). Txawm hais tias qhov sib txawv ntawm ob qho tib si platelet suav txo thiab MPV nce ntxiv nrog PRP, qhov kev hloov pauv ntawm platelet suav txo hauv WB tau ze li qhov tseem ceeb (310 ± 73 txog 286 ± 96 (-7.7%)); p = .06) thiab qhov nce hauv MPV yog qhov tseem ceeb (10.1 ± 0.5 + 10.8).Txawm hais tias qhov sib txawv ntawm ob qho tib si platelet suav txo thiab CVR ​​nce ntau dua nrog PRP, kev hloov pauv ntawm platelet suav poob hauv WB yuav luag tseem ceeb (310 ± 73 txog 286 ± 96 (-7.7%)); p = 0.06).увеличение MPV было значительным (los ntawm 10,1 ± 0,5 rau 11,8 ± 0,6 (+16,8) p < 0,001). qhov nce hauv MPV yog qhov tseem ceeb (ntawm 10.1 ± 0.5 txog 11.8 ± 0.6 (+ 16.8) p < 0.001).尽管PRP 在血小板计数减少和 MPV 增加方面的平均差异显着更大，但的WB 板计敾减小板计敾减（310 ± 73 至286 ± 96 (-7.7%)；p = .06）和MPV 的增加是显着的（10.1 ± 0.5 到11.8 ± 0.6 (+16.8) 1 p < . .尽管 PRP 在 血小板 计数 和 增加 方面的 平均 差异 显着的 大， 但 但 内血宏懰蠿是显着的（（（310 ± 73）至 286 ± 96 (-7.7%) ； p = .06）和MPV的增加是显着的（10.1 ± 0.5 p < 10.8）Kev hloov pauv hauv platelet suav txo hauv WB yog ze li qhov tseem ceeb (los ntawm 310 ± 73 rau 286 ± 96 (-7.7%)); p = 0.06), txawm hais tias PRP muaj qhov sib txawv loj dua ntawm platelet suav poob thiab MPV nce.thiab kev nce hauv MPV yog qhov tseem ceeb.(los ntawm 10,1 ± 0,5 до 11,8 ± 0,6 (+16,8) р < 0,001). (ntawm 10.1 ± 0.5 txog 11.8 ± 0.6 (+16.8) p < 0.001).
Qhov kawg concentration ntawm 20% qabzib yuav tsum pom qhov hloov pauv tseem ceeb hauv MPV, tab sis qhov kev hloov pauv hauv MPV tau tshaj tawm ntawm qhov kawg ntawm 25%.Platelet poob stabilized tom qab thawj zaug poob.Peb tau sau tseg qhov kev txo qis hauv CVR, txawm li cas los xij, CVR tau rov qab sai sai ntawm 25% cov piam thaj kawg, uas tau nce siab dua li qib CVR ​​pom ntawm qhov kawg ntawm cov piam thaj hauv 20% thiab 15% (Daim duab 3 thiab sab laug ntawm Table 3; cov thawv ntawv ntxoov ntxoo).qhia p-values ​​≤ alpha nrog Bonferroni kho ntawm 0.01).Kuj tseem muaj qhov poob qis hauv cov lej ntawm PLT, pom nyob rau hauv thawj theem ntawm 0-15 s, thiab tom qab ntawd tseem nyob ruaj khov (los ntawm 15 s txog 30 min; sab laug ntawm lub rooj 4).
Qhov sib ntxiv ntawm ntau qhov ntau ntawm cov piam thaj rau tag nrho cov ntshav ua rau thawj zaug txo qis hauv MPV tom qab los ntawm kev rov qab los ntawm ntau dua 20%.Cov lus dab neeg qhia qhov concentration ntawm qabzib tom qab dilution.D15, D20 thiab D25 tau ua hauv 1: 1 dilution.D21 thiab D41 tau ua tiav ntawm 1: 5 dilution.
Table 4 qhia txog kev hloov pauv hauv platelet suav thaum diluted hauv hypertonic qabzib.Peb tau pom qhov kev sib raug zoo ntawm koob tshuaj ntawm qhov poob tam sim ntawm PLT tus lej ntawm 1: 1 dilution thiab ntawm 1: 5 dilution.Muab piv rau 1: 1 dilutions raws li ib pawg nrog 1: 5 dilutions, pawg 1: 1 tau txo qis hauv platelet suav tam sim ntawd tsawg dua 1: 5 pawg 66 ± 48,000 (23%) piv rau 99 ± 69,000 (37%)., p = 0.014) hauv pawg 1:5.Tom qab thawj zaug poob ntawm thawj qhov ntsuas, cov platelet suav raws li feem pua ​​​​ntawm cov piam thaj stabilized (Fig. 4).
Thaum tag nrho cov ntshav ntxiv rau cov piam thaj hauv qhov sib piv 1: 1, platelet suav tau txo los ntawm 25%.Txawm li cas los xij, thaum tag nrho cov ntshav tau ntxiv ntawm qhov sib piv ntawm 1: 5, qhov txo qis ntau dua - txog 50%.
41% qabzib nce MPV sai dua thiab ntau dua li 25% lossis 21%.MPV cov txiaj ntsig tau pom nyob rau hauv daim duab 3. Ntawm tag nrho lwm cov dilutions, tsis pom qhov txo qis hauv MPV tam sim ntawd tom qab ntxiv 50% qabzib.Thaum siv 25% qabzib (glucose concentration 20.8% ntawm qhov kawg dilution), qhov kev hloov hauv MPV tau muab piv rau kev hloov pauv hauv 20% qabzib ntawm 1: 1 dilution (Fig. 3).Txawm hais tias kev hloov pauv hauv MPV tau pib ntau dua ntawm 41% sib xyaw ua ke ntau dua li ntawm 25%, qhov sib txawv ntawm MPV ntawm 41% thiab 25% tom qab 16 feeb tsis tseem ceeb (Table 3, txoj cai).Nws kuj tseem nthuav tias 25% qabzib nce MPV zoo dua li 20.8%.
Qhov no hauv vitro txoj kev tshawb fawb ib nrab tau lees paub peb qhov kev xav. Nws pom tau tias muaj peev xwm platelet lysis ib nrab los ntawm dextrose admixture, kev pabcuam sai ntawm cov platelets rau qhov hnyav hypertonicity, thiab qhov tseem ceeb ntawm MPV hauv cov lus teb rau> 25% concentrations ntawm hypertonic dextrose. Nws pom tau tias muaj peev xwm platelet lysis ib nrab los ntawm dextrose admixture, kev pabcuam sai ntawm cov platelets rau qhov hnyav hypertonicity, thiab qhov tseem ceeb ntawm MPV hauv cov lus teb rau> 25% concentrations ntawm hypertonic dextrose. Он показал потенциальный частичный лизис тромбоцитов примесью декстрозы, быструю аккомодацию тром боците тонуса и значительное повышение MPV в ответ на гипертоническую концентрацию декстрозы > 25%. Nws pom tau tias muaj peev xwm ntawm platelet lysis ib nrab nrog dextrose, ceev platelet kev pab rau siab heev hypertonicity, thiab ib qho tseem ceeb nce nyob rau hauv MPV teb rau hypertonic dextrose qib> 25%.它显示出通过葡萄糖混合物潜在的部分血小板溶解，血小板快速适应极端鍔带的高渗葡萄糖时 MPV 显着上升.它 显示 出 通过 葡萄糖 潜在的部分 血小板 溶解 血小板 快速 适应 极縫嫖 5浓度 高渗 葡萄糖 时时 mpv 显着。。。。 Он показывает потенциальный частичный лизис тромбоцитов смесями с глюкозой, быструю адаптацию тром боцин тонусу и значительное увеличение MPV в ответ на концентрацию гипертонической глюкозы > 25%. Nws qhia tau hais tias muaj peev xwm platelet lysis ib nrab los ntawm cov piam thaj sib xyaw, platelet hloov mus rau qhov hnyav hypertonicity, thiab kev nce ntxiv hauv MPV hauv cov lus teb rau hypertonic qabzib> 25%.Qhov pib nce siab tshaj plaws ntawm 41.6% qabzib raug, tab sis qhov nce hauv MPV nce mus txog 25% cov piam thaj raug kwv yees li 20 feeb tom qab kis tau.
Qhov concentration ntawm platelets cuam tshuam los ntawm cov piam thaj.Peb pom tias tus nqi ntawm PLT txo qis ntawm tag nrho cov kua qabzib dilutions.Kev poob qis ntawm cov platelets hauv H2O (0%) dilutions ntawm PRP series yuav cuam tshuam nrog osmotic lysis.Xwb, qhov no tuaj yeem yog cov khoom cuav uas tshwm sim los ntawm platelet clumping, tab sis qhov no yog qhov sib piv rau qhov tsis muaj MPV hloov ntawm qhov dilution.Qhov kev tshawb pom no txhais tau hais tias qee cov platelets yog rhiab heev rau hypoosmolarity.
Nyob rau hauv tag nrho 1: 1 dilutions ntawm qabzib, tus nqi ntawm PLT txo los ntawm 20-30%, txawm tias los ntawm D5W (hypotonic ntawm 252 mOsm), uas yuav qhia tau hais tias ib tug tshwj xeeb uas tsis yog-osmotic nyhuv ntawm qabzib, txij li thaum PLT thiab MPV tseem tsis tau hloov ntawm ib tug peb-fold nce nyob rau hauv concentration.qabzib.los ntawm D5W rau D25W.Qhov tseeb, PLT concentration tau nce me ntsis nrog nce osmolarity.
Qhov txo qis hauv PLT ntawm 1: 1 thiab 1: 5 dilutions txhais tau hais tias qhov kev sib cais yog nyob ntawm qhov pib thiab qhov kawg ntawm cov piam thaj.Yog tias nws tsuas yog nyob ntawm qhov pib concentration, ces ib tus yuav cia siab tias yuav pom qhov sib txawv hauv PLT txo ntawm 1: 1 concentrations.Tab sis peb tsis ua.Yog tias cov nyhuv lysis tsuas yog nyob ntawm qhov kawg ntawm cov piam thaj, ces peb tsis xav tias ntau qhov sib txawv ntawm 20% 1: 1 dilution thiab 20.8% 1: 5 dilution.Thiab tseem peb tau ua nws.
Yog tias platelet poob tshwm sim vim platelet lysis, ib feem lysate yog tsim, tom qab ntawd cytokines thiab cov yam ntxwv loj hlob raug tso tawm rau hauv ib puag ncig extracellular.Ntau qhov kev tshawb fawb tau pom tias platelet lysate yuav luag zoo li PRP raws li kev daws teeb meem loj [11].PRP nws tus kheej tau pom tias yog ib qho kev daws teeb meem zoo rau kev kho mob ntawm kev loj hlob [12-14].
Inactive platelets circulate nyob rau hauv daim ntawv ntawm ib tug disk ntxiv zog nrog ob peb sab hauv.Thaum lub sijhawm ua haujlwm, lawv ua rau ntau lub kheej kheej lossis amoeba zoo li, ua rau muaj qhov ntim ntau ntxiv.Qhov nce hauv ntim yuav tsum muaj qhov nce ntawm thaj chaw saum npoo, uas yog qhov tshwm sim ntawm extrusion ntawm qhov qhib tubule system (OCS) thiab ntxiv ntawm exocytic granules rau daim nyias nyias.Nws tseem yuav tsum tau txiav txim siab seb qhov nce hauv MPV vim yog hypertonic qabzib cuam tshuam nrog ib lossis ob qho tib si ntawm cov txheej txheem no, tab sis yog tias tom kawg, qhov nce hauv MPV yuav qhia tias degranulation.
Txoj kev tshawb no pom tau hais tias kev raug cov piam thaj ntau ntawm PRP lossis tag nrho cov ntshav platelets ua rau MPV nce hauv 15 feeb nrog cov piam thaj ntau ntawm 25% thiab 41.6%, feem.
Qhov nce hauv platelet MPV tuaj yeem yog vim qhov nthuav dav ntawm cov microtubule tangles nyob ib puag ncig hauv kev teb rau calcium influx.Liu et al.Glucose tau pom tias los kho cov calcium nkag los ntawm platelet TRPC6 channel [6].Peb qhov kev xav yog tias cov piam thaj ua rau muaj kev so ntawm microtubule tangles, ua rau muaj kev nce hauv MPV thiab platelet rhiab heev thiab / lossis ua kom muaj zog.Txawm li cas los xij, txiav txim los ntawm peb cov txiaj ntsig, qhov no tsuas yog ib feem ntawm zaj dab neeg.Hauv peb qhov kev ntsuam xyuas, tsis muaj concentration hauv qab D25W ua rau muaj kev nce hauv MPV.Muab hais tias peb tsis tau kuaj pom cov piam thaj hauv nruab nrab ntawm 12.5% ​​​​thiab 25%, peb theem 1 cov txiaj ntsig tau qhia tias yuav muaj qhov pib ntawm qhov ntau ntawm cov piam thaj ntau uas ua rau muaj kev nce hauv MPV.Kev sim ntxiv hauv theem 3 thiab 4 tau pom tias 20-25% qabzib zoo li yog qhov pib rau qhov no, tab sis nws tseem tsis paub meej vim li cas.
Peb kuj tau pom qhov txo qis hauv MPV ~ 9% tom qab centrifugation.Nws tsis yog tseeb seb qhov no txo ​​qis hauv MPV yog vim qhov loj dua thiab denser Platelets daig hauv RBC txheej ntawm Centrifuge.Qhov kev soj ntsuam no yuav yog qhov tseem ceeb rau cov kws kho mob vim nws tuaj yeem txhais tau tias PRP platelets yog ib qho me me thiab tsawg dua ntawm WB platelets.
Hauv kev tshawb fawb yav dhau los, peb tau pom tias kev npaj PRP los ntawm phau ntawv qhia yog pheej yig [8].Yog tias cov piam thaj nkag mus rau cov ntaub so ntswg platelets lossis PRP, ua rau lawv muaj kev cuam tshuam rau kev ua kom muaj zog, lossis yog tias PRP raug tsim nrog ib feem ntawm cov lysate, qhov no tuaj yeem txhim kho kev tsim dua tshiab thiab txo qhov xav tau kev kho.Yog li ntawd, kev sib xyaw ua ke ntawm PRP thiab cov piam thaj ntau ntau yuav raug nqi ntau dua li PRP lossis qabzib ib leeg.
Peb txoj kev tshawb fawb muaj ob peb qhov tsis txaus.Ua ntej, peb siv PRP tau los ntawm ntau txoj kev sib txawv.Qhov no tuaj yeem ua rau muaj kev tsis sib haum xeeb.Qhov thib ob, peb tsis tuaj yeem ua qhov kev tshuaj ntsuam biochemical ntawm ib qho ntawm peb cov qauv kom txiav txim siab ntau dua yog tias platelet activation tau tshwm sim.Peb xav ntsuas P-selectin, platelet factor 4, monocytic platelet aggregates, lossis lwm yam cim ntawm platelet activation kom nkag siab zoo dua qhov degree lossis muaj alpha granule degranulation, tab sis qhov no dhau ntawm qhov kev tshawb fawb no.Qhov thib peb, peb tsis tuaj yeem lees paub los ntawm electron microscopy lossis lwm txoj hauv kev uas qhov kev nce hauv MPV hauv cov piam thaj hauv cov platelets yog vim muaj kev cuam tshuam ntawm microtubule tangles.
Kev sib xyaw ntawm WB lossis PRP nrog 25% qabzib nce MPV, qhia txog qhov pib ntawm platelet activation, txawm hais tias txoj kev tshawb no tsis tau qhia txog kev nce qib ntawm kev sib sau lossis degranulation.Qhov sib xyaw ntawm cov piam thaj hypertonic ua rau platelet poob, tej zaum sawv cev rau cov nyhuv lytic.Kev ua kom ib nrab lossis lysis ntawm platelets tuaj yeem ua rau cov ntaub so ntswg rov ua dua tom qab txhaj tshuaj platelet.Nws tsis paub meej tias qhov tshwm sim ntawm kev kho mob li cas cov kev hloov pauv no tuaj yeem ua rau.Cov kev tshawb fawb ntxiv tau pom tias muaj kev ntsuas ntau dua ntawm kev ua kom muaj zog lossis lysis thiab tau soj ntsuam qhov sib txawv ntawm kev kho mob ntawm hypertonic qabzib sib xyaw nrog WB lossis PRP.
Glucose proliferative therapy yog ib qho yooj yim thiab pheej yig regenerative therapy uas nthuav dav thiab txhawb kev tshawb fawb soj ntsuam.Txoj kev tshawb no qhia txog kev siv lub cev lub cev uas, yog tias paub tseeb, tuaj yeem pab peb nkag siab txog ib feem ntawm cov txheej txheem rov ua dua tshiab ntawm kev kho mob proliferative.
Biomedical thiab Health Informatics ntawm University of Missouri, Kansas City School of Medicine, Kansas City, USA
Tib neeg Cov Ntsiab Lus: Txhua tus neeg koom hauv txoj kev tshawb fawb no tau muab lossis tsis pom zoo.Lub Koom Haum Thoob Ntiaj Teb rau Cov Tshuaj Cellular tau tso cai ICMS-2017-003.Cov txheej txheem hauv qab no tau pom zoo siv ntxiv los ntawm Lub Tsev Haujlwm Saib Xyuas Kev Tshawb Fawb ntawm International Society for Cellular Medicine: Title: Kev suav cov tshuaj platelet-nplua nuj plasma yield raws li cov hauv paus ntsiab lus CBC platelet suav.Tsiaj Yam: Txhua tus kws sau ntawv tau lees paub tias tsis muaj tsiaj lossis cov ntaub so ntswg koom nrog hauv txoj kev tshawb no.Kev tsis sib haum xeeb ntawm kev txaus siab: Raws li ICMJE Uniform Disclosure Form, txhua tus kws sau ntawv tshaj tawm cov lus hauv qab no: Cov ntaub ntawv them nyiaj / kev pabcuam: Txhua tus kws sau ntawv tshaj tawm tias lawv tsis tau txais kev txhawb nqa nyiaj txiag los ntawm ib lub koom haum rau kev xa mus ua haujlwm.Kev Sib Raug Zoo Nyiaj Txiag: Txhua tus kws sau ntawv tshaj tawm tias lawv tsis tam sim no lossis hauv peb lub xyoos dhau los muaj kev sib raug zoo nyiaj txiag nrog rau ib lub koom haum uas yuav nyiam ua haujlwm.Lwm Cov Kev Sib Raug Zoo: Txhua tus kws sau ntawv tshaj tawm tias tsis muaj lwm yam kev sib raug zoo lossis kev ua ub no uas yuav cuam tshuam rau cov haujlwm xa mus.
Harrison TE, Bowler J, Reeves K et al.(Tsib Hlis 17, 2022) Cov txiaj ntsig ntawm cov piam thaj ntawm platelet suav thiab ntim: cuam tshuam rau cov tshuaj kho dua tshiab.Kho 14(5): e25081.doi: 10.7759/cureus.25081
© Copyright 2022 Harrison et al.Qhov no yog ib tsab xov xwm qhib tau muab faib raws li cov ntsiab lus ntawm Creative Commons Attribution License CC-BY 4.0.Unlimited siv, faib, thiab luam tawm nyob rau hauv ib qho nruab nrab yog tso cai, yog tus thawj sau thiab qhov chaw yog credit.