“Ya adịla mgbe obi abụọ adịghị ya na obere ìgwè ụmụ amaala nwere echiche, raara onwe ha nye pụrụ ịgbanwe ụwa.N’ezie, ọ bụ naanị ya ebe ahụ.”
Ọrụ Cureus bụ ịgbanwe usoro mbipụta ahụike ogologo oge, nke ntinye nyocha nwere ike ịdị oke ọnụ, mgbagwoju anya ma na-ewe oge.
Platelet bara ụba plasma/prp, mmeghari anụ ahụ, ịgbalite platelet, ọgwụgwọ ịgbasa glucose, platelet, ọgwụgwọ ịgbasa.
Kpọtụrụ akụkọ a dịka: Harrison TE, Bowler J, Reeves K, et al.(Mee 17, 2022) Mmetụta nke glucose na ọnụ ọgụgụ platelet na olu: ihe ọ pụtara maka ọgwụ na-emegharị ahụ.Ọgwụgwọ 14 (5): e25081.doi:10.7759/cureus.25081
Platelet-rich plasma (PRP) na hypertonic glucose ngwọta bụ nke a na-ejikarị maka ịgba ọgwụ na ọgwụ na-emegharị ahụ, mgbe ụfọdụ ọnụ.A kọpụtabeghị mmetụta nke glucose hypertonic na platelet lysis na ịgbalite ya na mbụ.Anyị nwalere mmetụta nke mkpokọta glucose dị elu na ọnụ ọgụgụ platelet na erythrocyte, yana mpịakọta cell na PRP na ọbara dum (WB).Mbelata ngwa ngwa nke ọnụ ọgụgụ platelet mere na ngwakọta glucose niile agwakọtara na PRP ma ọ bụ ọbara dum, dabara na akụkụ lysis. Mgbe nkeji mbụ gachara, ọnụ ọgụgụ platelet ka kwụsiri ike, na-atụ aro nnabata ngwa ngwa nke platelet fọdụrụnụ ruo oke hypertonicity (>2000 mOsm). Mgbe nkeji mbụ gachara, ọnụ ọgụgụ platelet ka kwụsiri ike, na-atụ aro nnabata ngwa ngwa nke platelet fọdụrụnụ ruo oke hypertonicity (>2000 mOsm). После первой минуты количество тромбоцитов онлайн ma ekstremalnoho (>2000 mmsm). Mgbe nkeji mbụ gachara, ọnụ ọgụgụ platelet ka kwụsiri ike, na-egosi nnabata ngwa ngwa nke platelet fọdụrụnụ ruo oke hypertonicity (>2000 mOsm).第一分钟后，血小板计数保持稳定，表明残余血小板迅速适应极端（> 2000 mOsm.2000 mOsm）高渗状态。 После первой минуты количество тромбоцитов эkstremalnomu (> 2000 mmsm) гиperosmolyarnomy sostoyt. Mgbe nkeji mbụ gachara, ọnụ ọgụgụ platelet ka kwụsiri ike, na-egosi ngbanwe ngwa ngwa nke platelet fọdụrụnụ na steeti hyperosmolar dị oke (>2000 mOsm).Mmụba glucose dị 25% na karịa butere mmụba dị ukwuu na mkpokọta platelet (MPV), na-egosi mmalite mmalite nke ịgbalite platelet.Achọkwu ọmụmụ ihe iji chọpụta ma platelet lysis ma ọ bụ ịgbalite na-eme yana ma hypertonic glucose injection naanị ma ọ bụ yana njikọta na PRP nwere ike inyekwu uru ụlọ ọgwụ.
N'afọ ndị 1950, dọkịta na-awa America bụ George Hackett chọpụtara na ọ nwere ike ịkwụsị nkwonkwo na azụ mgbu n'ọtụtụ ndị ọrịa site n'itinye ngwọta na-agbasawanye n'ime akwara na akwara.Nnwale ya na oke bekee gosiri na ọgwụgwọ ahụ, nke ọ kpọrọ ọgwụgwọ proliferative, mere ka akwara na-amụba ma na-ewusi ike.Ọmụmụ akụkọ ihe mere eme ekwenyela na a na-emepụta collagen ọhụrụ n'oge usoro a [1].
N'ime iri afọ ole na ole mbụ, a nwalere ọtụtụ ụzọ nkesa dị iche iche.Ka ọ na-erule n'afọ 1990, ọtụtụ ndị na-eme ihe na-ewere oke glucose dị ka ụzọ kachasị mma na nke kachasị dị irè.Agbanyeghị, usoro ihe omume ka edoghị anya.
Ole na ole ọmụmụ ụlọ ọgwụ ka emere na narị afọ nke 20 na-esote ọrụ Hackett.Otú ọ dị, n'ime afọ 2000 enwere mmasị ọhụrụ na ọtụtụ ule ụlọ ọgwụ na-aga nke ọma nke ọgwụgwọ proliferative agwụla maka ọgwụgwọ nke mgbu azụ dị ala [2], osteoarthritis nke ikpere [3], na epicondylitis mpụta [4].
Mweghachi anụ ahụ chọrọ ntinye nke sel sel.Ya mere, n'ụzọ ụfọdụ, nnukwu mkpokọta glucose kwesịrị ịkpata mbugharị, mmụgharị, na ọdịiche nke sel stem.Anyị na-eche na platelet nwere ike na-arụ ọrụ dị ka ndị ozi yana ọkwa glucose dị elu nwere ike ime ka platelet hapụ cytokines na ihe ndị na-eto eto, si otú ahụ na-akwalite usoro nhazigharị, karịsịa ịkwaga sel sel gaa n'ebe ọkwa glucose dị elu.
Mgbalite platelet na-ebutekarị mmụba nke calcium intracellular [5].Liu et al.na 2008 gosiri na ọkwa glucose dị elu na-abawanye ọrụ nke ọwa 6 (TRPC6) nke na-anabata ihe na-adịru nwa oge na akpụkpọ ahụ plasma, nke na-eduga na ntinye nke ion calcium n'ime platelet [6].Nnyocha ọzọ gosiri na mkpughe nke mpaghara akụkụ microtubule na ion calcium na-eme ka ntụrụndụ, mgbasawanye, na nrụrụ nke mpaghara dị n'akụkụ, nke n'aka nke ya na-eme ka mgbanwe dị na diski gaa na gburugburu, na-eme ka ụda platelet pụta (MPV) [7].
Echiche anyị n'ọmụmụ ihe a bụ na mkpughe nke platelet na ọkwa glucose dị elu na-emetụta mpaghara akụkụ microtubule na gburugburu intracellular, na-ebute mmụba na MPV.
Ndị niile sonyere bịanyere aka n'akwụkwọ nkwenye ama ama ka akọwara nkọwa nke ọmụmụ ahụ na tupu ịnata ihe nlele ahụ.N'ime ọmụmụ ihe a, a na-eji naanị ihe nlele PRP nwere hematocrit karịrị 2% ka e wee nwee ike ịgụnye ọnụ ọgụgụ erythrocyte (erythrocyte) na ọnụ ọgụgụ corpuscular nke mkpụrụ ndụ ọbara uhie (MCV) maka ntụnyere.
A na-eduzi ọmụmụ ihe na akụkụ anọ, akụkụ mbụ bụ PRP na akụkụ ndị fọdụrụ bụ ọbara dum (Table 1).Dịka akọwara na mbụ [8], a gbakọrọ ndị agha centrifugal niile (RCF, g-force) site na etiti (Rmid, na cm) nke kọlụm ọbara dị na sirinji centrifugal.Anyị họọrọ iji MPV dị ka ihe nrịbama nke mgbata platelet na ọnụ ọgụgụ platelet dị ka ihe na-egosi lysis platelet nwere ike, nke enwere ike tụọ ha abụọ n'ụzọ dị mfe na ndị na-enyocha hematology ọkọlọtọ.
N’akụkụ nke mbụ, ndị ọrụ afọ ofufo 47 nyere ihe nlele ọbara—otu tube nke ethylenediaminetetraacetic acid (EDTA) na otu ihe nlele ọbara zuru ezu nke PRP (nke e ji sodium citrate eme ihe (NaCl, 3%)) (Table 1).Tinye rocker na tube ozugbo.A na-eme ọnụ ọgụgụ ọbara zuru ezu (CBC) na ihe nlele EDTA na ugboro atọ, na nyocha nke NaCl na nke atọ maka nyocha CBC, wee kwadebe PRP site na ụzọ dị iche iche akọwara n'elu [8].A kwadebere ihe nlele PRP niile site na centrifugation na 900-1000 g.Gwakọta ihe nlele PRP ọ bụla na igwekota vortex maka 5-10 sekọnd, wee kewaa 0.5 ml aliquots ise n'ime tubes.
Iji nyochaa mmetụta nke mkpughe platelet na mkpokọta glucose dị elu, nha nha (0.5 ml) nke 0%, 5%, 12.5%, 25% na 50% glucose na mmiri ka agwakọtara ya na ihe nlele platelet iji nweta 0%, 2.5% 6.25%, 12.5% ​​na 25% nke ngwakọta nke tuber maka nkeji 5.A tụlere TAC nke ngwakọta ọ bụla n'ime ugboro atọ ka nkeji iri na ise gachara.Ọnụ ọgụgụ platelet (PLT), ọnụ ọgụgụ RBC, MCV, na MPV bụ nkezi maka tube nke ọ bụla, yana ọnụ ọgụgụ platelet, ọnụ ọgụgụ RBC, MCV, na MPV ka agbakọrọ maka nlele PRP niile.
Mgbe akụkụ nke mbụ nke nchịkọta data gwụchara, anyị chọpụtara mmụba dị ukwuu na olu platelet na platelet PRP mgbe agbakwunyere D50W.PRP platelet adịghị anọchi anya platelet niile dị n'ọbara, na usoro PRP dị iche na nke WB.Ya mere, anyị kpebiri ime ule nke abụọ nke mmetụta nke ịgbakwunye D50W na ọbara dum.
Maka agba nke abụọ, anyị họọrọ ihe nlele nke 30 dabere na nsonaazụ sitere na usoro nke mbụ, dị ka akọwara na ngalaba Analysis.N'usoro isiokwu a, ndị ọrụ afọ ofufo 20 nyere ihe nlele ọbara (Table 1).A dọbara ọbara zuru oke (1.8 ml) n'ime sirinji 3 ml wee were 0.2 ml 40% NaCl kpuchie ya.A na-agwakọta sirinji ọbara niile maka sekọnd ise na igwekota vortex na CBC nyochara ya na ugboro atọ.Mgbe nyochachara, a na-agbakwunye ọbara anticoagulated na 2 ml nke 50% glucose na sirinji 5 ml (ntụgharị glucose ikpeazụ bụ ihe dịka 25% (D25) ma tinye ya na tube shake maka nkeji 30. Mgbe nkeji 30 gasịrị, a na-enyocha D25/CBC na sirinji WB n'ime syringe. Platelet count, RBC, mean MPVsy, RBC count, RBC count, RBC per MC. A na-agbakọ V, na MPV maka nlele ọ bụla tupu na mgbe agbakwunyere glucose.
Ebe ọ bụ na platelet dị n'ọbara dum na-enwekarị hypertonic glucose n'oge ọgwụgwọ glucose na-amụba n'ihi obere ịgba ọgwụ mgbochi, na ọ bụghị ihe a na-ahụkarị ijikọta PRP na hypertonic glucose tupu ịgba ọgwụ, anyị kpebiri ịmụ hypertonic glucose na WB na Nkebi 1. Nzọụkwụ atọ na anọ.Na ọkwa nke ọ bụla, ndị ọrụ afọ ofufo 20 nyere 7-8 ml ACD-A (acid nwere trisodium citrate (22.0 g/l), citric acid (8.0 g/l) na glucose (24.5 g/l), ngwọta dextrose citrate) maka ọgwụ mgbochi ọbara (Table 1).Naanị ngwakọta nke glucose karịrị 12.5% ​​ka ejiri iji chọpụta pasenti ọnụ ụzọ jikọtara na mmụba nke MPV.Na ọkwa nke atọ, a na-etinye 1 ml nke ọbara n'ime tube nyocha.Mgbe ahụ gwakọta ọbara na igwekota vortex maka sekọnd 10 site n'ịgbakwunye 1 ml nke 30% glucose, 40% glucose, ma ọ bụ 50% glucose na tube iji nweta mkpokọta glucose ikpeazụ nke 15%, 20%, na 25%, n'otu n'otu.A na-enyocha ihe nlele ọbara glucose maka CBC ozugbo agwakọtachara ma na-emegharị kwa nkeji abụọ ọ bụla maka nkeji iri atọ.
N'oge ngwakọta mbụ, mgbakwunye nke 1: 1 hypertonic glucose na WB ma ọ bụ PRP na-ekpughe platelet na mkpokọta n'elu 25% maka ọtụtụ sekọnd.N'ime usoro nke anọ, iji nyochaa mmetụta nke hypertonic hypertonic na obere mkpokọta mbido mbụ wee nwalee oke nke mmetụta glucose, anyị gbakwunyere naanị ntakịrị ọbara na D25W ma ọ bụ D50W.Tinye 1 ml nke D25W ma ọ bụ D50W n'ime tube ma tinye 0.2 ml nke WB ka ị na-atụgharị ihe nlele ahụ maka 10 sekọnd.N'okwu ndị a, a na-ekpughere ọbara na glucose na mkpokọta ihe dịka 20% karịa ntinye ikpeazụ, karịa 50% karịa ntinye ikpeazụ dịka na nkeji 3, na-ebute mkpokọta glucose ikpeazụ nke 20.8% na 41.6%.A tụlere ihe atụ agwakọtara n'otu oge dịka nke dị na nzọụkwụ 3.
Na nzọụkwụ mbụ nke usoro mmịkọ glucose ọ bụla, a na-ewere ihe nlele 30 n'ihi na nke a bụ nha kwesịrị ekwesị maka ọmụmụ ihe pilot [9].N'ọgwụgwụ nke usoro ọ bụla (gụnyere akụkụ nke mbụ), nyochaa ntozu nke nha nlele site na iji usoro ejiri mee ka ọnụọgụ nlele dị mkpa iji chọpụta n'ụzọ na-agbanwe agbanwe na-aga n'ihu n'otu ọnụ ọgụgụ mmadụ.Formula n = Z2 x SD2/E2.Na ngụkọ a, Z bụ akara Z-score, SD bụ ọkọlọtọ ọkọlọtọ, na E bụ njehie achọrọ [10].Alfa anyị bụ 0.05, nke dabara na uru Z nke 1.96, anyị na-atụkwa anya njehie nke 5 (na pasent).N'ihi ya, anyị na-edozi maka n = (1.962 x SD2)/52.Nsonaazụ gosiri na nha nlele achọrọ maka ọkwa ọ bụla pere mpe karịa ọnụ ọgụgụ ndị anakọtara.
N'ime oge 1, 3 na 4 na-eji ihe karịrị otu mkpokọta glucose, a na-enyocha mmetụta nke mkpokọta glucose dị iche iche site n'ịtụle mgbanwe dị nta n'etiti oge 0 na oge ọ bụla sochiri (usoro 1 na nkeji 15, oge 3 na nkeji 15).na anọ na 15 sekọnd, mgbe ahụ kwa nkeji abụọ ọ bụla.) A na-atụnyere mgbanwe mgbanwe maka oge ọ bụla site na iji Mann-Whitney U-ule n'ihi na data adịghị agbaso nkesa nkịtị dị ka nyocha nke Shapiro-Wilk kwadoro.Ebe ọ bụ na a na-eme nyocha 1-na-1 nke ọtụtụ otu (ise) na nke mbụ, nke atọ na nke anọ (ise na ngụkọta), a na-emezi Bonferroni iji dozie uru alfa chọrọ na ≤0.01 ma ọ bụghị ≤0.05.
Mbelata ọnụ ọgụgụ platelet na ntinye niile nke hypertonic dextrose na mmụba nke MPV na PRP platelets na> 12.5% ​​dextrose mkpokọta: ọnụ ọgụgụ PRP platelet bilitere site n'otu oge ruo ugboro ise itinye uche ma e jiri ya tụnyere ọbara dum, na-agbanwe site na usoro (anaghị egosipụta ya). Mbelata ọnụ ọgụgụ platelet na ntinye niile nke hypertonic dextrose na mmụba nke MPV na PRP platelets na> 12.5% ​​dextrose mkpokọta: ọnụ ọgụgụ PRP platelet bilitere site n'otu oge ruo ugboro ise itinye uche ma e jiri ya tụnyere ọbara dum, na-agbanwe site na usoro (anaghị egosipụta ya). Уменьшение количества тромбоцитов при всех концентрациях гипертонической декстрозы и увеличение MPV. раци декстрозы > 12.5%: количество тромбоцитов PRP увеличилось в 1-5 раз по сравнению с метода (adịghị mma). Mbelata ọnụ ọgụgụ platelet na mkpokọta hypertonic dextrose niile yana mmụba MPV na platelet PRP na> 12.5% ​​ịta ahụhụ dextrose: ọnụ ọgụgụ PRP platelet mụbara ugboro 1-5 ma e jiri ya tụnyere ọbara zuru oke, dabere na usoro (egosighi). ).> 12.5% ​​的 的 浓度 浓度 浓度 浓度 浓度 血小板 血小板 m mpv 增加: 与 基线 血小板 血小板 血小板 1 上升 上升 1 倍 (未 方法). Na> 12.5% ​​glucose mkpokọta, nnukwu mkpokọta glucose na-ebelata ọnụ ọgụgụ ọbara, PRP ọbara MPV na-abawanye: ma e jiri ya tụnyere 与基线全血, ọnụ ọgụgụ ọbara PRP na-abawanye site na 1 ruo 5 ugboro karịa nke itinye uche (anaghị akọwa ya). При концентрациях глюкозы >12.5% ​​все концентрации мбоцитах PRP: количество тромбоцитов PRP увеличивалось от 1- 5-кратныh ой крови, в зависимости от метода (nе описано ). Na mkpokọta glucose> 12.5%, mkpokọta glucose ọbara mgbali na-ebelata ọnụ ọgụgụ platelet yana ịbawanye MPV na platelet PRP: ọnụ ọgụgụ PRP platelet mụbara 1 ruo 5 ugboro ma e jiri ya tụnyere ntọala ọbara dum, dabere na usoro (dị ka akọwara).Ọgụgụ 1 na-egosi na ọnụ ọgụgụ platelet belatara ihe fọrọ nke nta ka ọ bụrụ 75% mgbe a gbasasịrị ya na mmiri yana site na 20-30% mgbe nkeji iri na ise gachara ya na ọkwa glucose dị iche iche ma e jiri ya tụnyere PRP baseline na 1: 1 dilution emeziri maka olu (1-k1 na mgbazi olu).k -1 ozuzu).1 ozuzu).
A na-egosipụta ọnụọgụ mkpụrụ ndụ dị na dilution ọ bụla dị ka akụkụ dị nta nke nọmba mbụ tupu ntughari.
MPV gbadara ntakịrị n'oge mmepụta PRP, na-enweghị mgbanwe ọzọ na ntinye dilution na 12.5% ​​na mmiri ma ọ bụ glucose (gụnyere 25% PRP glucose ngwakọta) wee mụbaa karịa 20% mgbe ọ gbasasịrị na 50% glucose ngwọta (Fig. .2).).N'ụzọ dị iche, erythrocytes egosighị mgbanwe dị ịrịba ama n'ime olu na nfefe ọ bụla ọzọ karịa H2O.
A na-egosipụta nkezi olu nke mkpụrụ ndụ n'ime mgbaze ọ bụla dị ka pasent nke olu mbụ tupu ntughari.
Mbelata yiri nke ahụ mana nke a na-akpọchaghị ọnụ na ọnụ ọgụgụ platelet yana mmụba na CVR na BC kpughere glucose 50% (iji mepụta ya na 25% glucose).Tebụl 2 na-atụnyere ọnụọgụ cell na mpịakọta cell n'ime ọbara dum agbazere na 50% dextrose na nkeji 1 PRP data agbazere na 50% dextrose.Mgbanwe na ọnụ ọgụgụ RBC na RBC MCV abụghị ihe doro anya na ọ bụghị ihe anyị lekwasịrị anya.
SD = ọkọlọtọ ọkọlọtọ, MD = pụtara ọdịiche dị n'etiti otu, SE = ọkọlọtọ nke pụtara ọdịiche, RBC = erythrocytes, PLT = platelet, PRP = platelet ọgaranya plasma, WB = ọbara dum.
Mgbe agbakwunyere D50W na WB, mbelata nke dilution-gbanwee platelet ọnwụ bụ 7.7% (310 ± 73 vs. 286 ± 96) ma e jiri ya tụnyere 17.8% maka dilution PRP na D50W (664 ± 348 vs. 544 ± 277).MPV WB mụbara site na 16.8% (site na 10.1 ± 0.5 ruo 11.8 ± 0.6), ebe MPV PRP mụbara site na 26% (9.2 ± 0.8 vs. 11.6 ± 0. 7). Ọ bụ ezie na ọdịiche pụtara ìhè na mbelata ọnụ ọgụgụ platelet na mmụba MPV bụ nke ukwuu na PRP, mgbanwe na mbelata ọnụ ọgụgụ platelet n'ime WB fọrọ nke nta ka ọ bụrụ ihe dị ịrịba ama (310 ± 73 ruo 286 ± 96 (-7.7%); p = .06) na mmụba na MPV dị ịrịba ama (10.1 ± 0.5 ruo 11.8) <+ 0.0.8). Ọ bụ ezie na ọdịiche pụtara ìhè na mbelata ọnụ ọgụgụ platelet na mmụba MPV bụ nke ukwuu na PRP, mgbanwe na mbelata ọnụ ọgụgụ platelet n'ime WB fọrọ nke nta ka ọ bụrụ ihe dị ịrịba ama (310 ± 73 ruo 286 ± 96 (-7.7%); p = .06) na mmụba na MPV dị ịrịba ama (10.1 ± 0.5 ruo 11.8) <+ 0.0.8).Ọ bụ ezie na ọdịiche pụtara ìhè na mbelata ọnụ ọgụgụ platelet na mmụba CVR ka ukwuu na PRP, mgbanwe na ọnụ ọgụgụ platelet mbelata n'ime WB fọrọ nke nta ka ọ dị ịrịba ama (310 ± 73 ruo 286 ± 96 (-7.7%); p = 0.06).увеличение MPV было значительным (от 10,1 ± 0,5 na 11,8 ± 0,6 (+16,8) p <0,001). mmụba na MPV dị ịrịba ama (site na 10.1 ± 0.5 ruo 11.8 ± 0.6 (+16.8) p <0.001).尽管PRP 在血小板计数减少和MPV 增加方面的平均差异但显着的（310 ± 73 至286 ± 96 (-7.7%)；p = .06）和MPV 的增加是显着的（10.1 ± 0.5 到11.8) <+0.8 ± 0.8尽管 PRP 在 血小板 计数 和 和 增加 方面 的 平均 差异 显着 大乎 是 显着 的 (（310 ± 73 至 286 ± 96 (-7.7%) ； p = .06）和MPV 的增加是显着的（10.5 .001).Mgbanwe nke mbelata ọnụ ọgụgụ platelet n'ime WB fọrọ nke nta ka ọ dị ịrịba ama (site na 310 ± 73 ruo 286 ± 96 (-7.7%); p = 0.06), n'agbanyeghị na PRP nwere nnukwu pụtara ọdịiche dị na ọnụ ọgụgụ platelet mbelata na mmụba MPV.na mmụba nke MPV dị ịrịba ama.(nke 10,1 ± 0,5 na 11,8 ± 0,6 (+16,8) р <0,001). (site na 10.1 ± 0.5 ruo 11.8 ± 0.6 (+16.8) p <0.001).
A chọrọ ntinye ikpeazụ nke 20% glucose iji hụ mgbanwe dị ịrịba ama na MPV, mana mgbanwe MPV pụtara ìhè na njedebe ikpeazụ nke 25%.Mfu Platelet kwụsiri ike ka mbelata nke mbụ gasịrị.Anyị chọpụtara mbelata nke ukwuu nke mbụ na CVR, agbanyeghị, CVR weghachiri ngwa ngwa na ntinye glucose ikpeazụ nke 25%, nke dị elu nke ukwuu karịa ọkwa CVR hụrụ na mkpokọta glucose ikpeazụ nke 20% na 15% (Fig 3 na n'aka ekpe nke Tebụl 3; igbe mkpuchi).gosi p-ụkpụrụ ≤ alpha nwere mgbazi Bonferroni nke 0.01).Enwekwara mbelata mbido mbụ na ọnụ ọgụgụ PLT, nke a hụrụ na mbido mbụ nke 0-15 s, wee kwụsie ike (site na 15 s ruo 30 min; aka ekpe nke tebụl 4).
Mgbakwunye nke ọkwa glucose dị iche iche na ọbara dum butere mbelata mbido ngwa ngwa na MPV yana mgbake dabere na ntinye uche nke ihe karịrị 20%.Akụkọ ifo na-egosi ntinye nke glucose mgbe dilution gasịrị.A na-eme D15, D20 na D25 na 1:1 mgbaze.A na-eme D21 na D41 na 1: 5 dilution.
Tebụl 4 na-egosi mgbanwe nke ọnụ ọgụgụ platelet mgbe agbanyere ya na glucose hypertonic.Anyị hụrụ mmekọrịta dabere na dose n'etiti ọdịda ozugbo na nọmba PLT na 1: 1 dilution na 1: 5 dilution.N'ịtụle 1: 1 dilutions dị ka otu otu na 1: 5 dilutions, otu 1: 1 nwere mbelata ozugbo na ọnụ ọgụgụ platelet na-erughị 1:5 otu 66± 48,000 (23%) megide 99± 69,000 (37%)., p = 0.014) na otu 1:5 .Mgbe ọdịda mbụ na ebe nlele mbụ gasịrị, platelet na-agụta dị ka pasentị glucose kwụsiri ike (Fig 4).
Mgbe etinyere ọbara dum na glucose na nha 1: 1, ọnụ ọgụgụ platelet na-ebelata ihe dịka 25%.Otú ọ dị, mgbe agbakwunyere ọbara dum na nha nke 1: 5, mbelata ahụ dị ukwuu - ihe dịka 50%.
41% glucose mụbara MPV ngwa ngwa yana karịa 25% ma ọ bụ 21%.E gosiputara nsonaazụ MPV na eserese 3. N'ime ntụle ndị ọzọ niile, ọ nweghị mbelata mbido mbụ na MPV ka agbakwunyere 50% glucose.Mgbe ị na-eji 25% glucose (glucose ntinye 20.8% na dilution ikpeazụ), mgbanwe MPV dị ka mgbanwe nke 20% glucose na 1: 1 dilution (Fig 3).Ọ bụ ezie na mgbanwe ndị dị na MPV dị na mbụ na 41% mgbagwoju anya karịa na 25%, ọdịiche dị na MPV n'etiti 41% na 25% mgbe nkeji 16 gasịrị adịkwaghị mkpa (Table 3, right).Ọ dịkwa mma na 25% glucose mụbara MPV nke ọma karịa 20.8%.
Ọmụmụ ihe in vitro nke a gosipụtara n'ụzọ ụfọdụ n'echiche anyị. O gosiputara lysis platelet nwere ike site na dextrose admixture, nnabata ngwa ngwa nke platelet ruo oke hypertonicity, yana mmụba dị ukwuu na MPV na nzaghachi> 25% mkpokọta hypertonic dextrose. O gosiputara lysis platelet nwere ike site na dextrose admixture, nnabata ngwa ngwa nke platelet ruo oke hypertonicity, yana mmụba dị ukwuu na MPV na nzaghachi> 25% mkpokọta hypertonic dextrose. Оn pokazal potentsyalny chestychnыy lyzys trombotsytov prymesyu dexstrozы гипертонуса и значительное повышение MPV в ответ на гипертоническую концентрацию декстрозы> 25%. O gosiputara lysis platelet nwere ike na dextrose, ebe obibi platelet ngwa ngwa ruo oke hypertonicity, yana mmụba dị ukwuu na MPV na nzaghachi hypertonic dextrose ọkwa>25%.它显示出通过葡萄糖混合物潜在的部分血小板溶解，血小板快速适应极端，血小板快血小板快速递应极端，响应>不应>2的高渗葡萄糖时MPV 显着上升。它 显示 出 通过 葡萄糖 潜在 的 部分 血小板 溶解 血小板浓度 高渗 葡萄糖 时 时 mpv 显着….. Оn pokazыvaet potentsyalnыy lyzys trombotsytov smesyami s gulukoy у гипертонусу и значительное увеличение MPV в ответ на концентрацию гипертонической глюкозы > 25%. Ọ na-egosiputa mbelata nke platelet lysis site na ngwakọta glucose, ngbanwe ngwa ngwa platelet na oke hypertonicity, yana mmụba dị ukwuu na MPV na nzaghachi hypertonic glucose> 25%.Mmụba mbụ kachasị na mkpughe glucose 41.6%, mana mmụba nke MPV rutere nso 25% mkpughe glucose ihe dịka nkeji 20 ka ekpughere ya.
Mbelata nke platelet na-emetụta glucose.Anyị chọpụtara na ọnụọgụ PLT na-agbadata n'ụdị ọ bụla nke glucose.Mbelata dị nkọ na ọnụ ọgụgụ platelet na H2O (0%) mmịfe nke usoro PRP nwere ike jikọta ya na osmotic lysis.N'aka nke ọzọ, nke a nwere ike ịbụ ihe arụpụtara site na nchikota platelet, ma nke a dị iche na enweghị mgbanwe MPV n'oge dilution a.Nchọpụta a pụtara na ụfọdụ platelet na-enwe mmetụta nke ukwuu maka hypoosmolarity.
N'ime 1: 1 dilution nke glucose niile, ọnụọgụ PLT belatara site na 20-30%, ọbụlagodi site na D5W (hypotonic na 252 mOsm), nke nwere ike igosi nsonaazụ glucose na-abụghị osmotic, ebe ma PLT na MPV agbanwebeghị na mmụba okpukpu atọ na ntinye uche.glucose.site na D5W ruo D25W.N'ezie, ntinye uche PLT na-abawanye ntakịrị site na mmụba osmolarity.
Mbelata nke PLT n'etiti 1: 1 na 1: 5 dilutions pụtara na mmetụta mgbasa na-adabere na ntinye glucose mbụ na nke ikpeazụ.Ọ bụrụ na ọ dabere naanị na ntinye uche mbụ, mgbe ahụ, mmadụ ga-atụ anya ịhụ ọdịiche dị na Mbelata PLT n'etiti ntinye 1: 1.Ma anyị adịghị.Ọ bụrụ na mmetụta lysis na-adabere naanị na ntinye glucose ikpeazụ, mgbe ahụ anyị anaghị atụ anya nnukwu ọdịiche dị n'etiti 20% 1: 1 dilution na 20.8% 1: 5 dilution.Ma anyị mere ya.
Ọ bụrụ na mfu platelet na-apụta n'ihi platelet lysis, a na-emepụta lysate akụkụ, mgbe nke ahụ gasịrị, a na-ahapụ cytokines na ihe ndị na-eto eto na gburugburu ebe obibi extracellular.Ọtụtụ nnyocha egosiwo na platelet lysate fọrọ nke nta ka ọ dị irè dị ka PRP dị ka ngwọta mgbasawanye [11].E gosipụtara PRP n'onwe ya ka ọ bụrụ ngwọta dị irè maka ọgwụgwọ mgbasawanye [12-14].
platelet na-adịghị arụ ọrụ na-ekesa n'ụdị diski ejiri ọtụtụ ihe arụ ọrụ dị n'ime mesie ike.N'oge ịgbalite, ha na-enwe ọdịdị okirikiri ma ọ bụ amoeba, na-ebute mmụba nke olu.Mmụba nke olu na-achọ mmụba na mpaghara elu, nke bụ nsonaazụ nke extrusion nke usoro tubule na-emeghe (OCS) na mgbakwunye nke granules exocytic na akpụkpọ ahụ.A ka ga-ekpebi ma mmụba nke MPV nke glucose hypertonic kpatara gụnyere otu ma ọ bụ abụọ n'ime usoro ndị a, mana ọ bụrụ na nke ikpeazụ, mgbe ahụ mmụba na MPV ga-egosi mbibi.
Ọmụmụ ihe a gosiri na ikpughe ọkwa glucose dị elu na PRP ma ọ bụ platelet ọbara dum butere mmụba na MPV n'ime nkeji iri na ise yana ntinye glucose dị 25% na 41.6% n'otu n'otu.
Mmụba nke platelet MPV nwere ike ịbụ n'ihi mgbasa nke microtubule tangles gbara ya gburugburu na nzaghachi na ntinye calcium.Liu et al.E gosiputara glucose ka ọ na-eme ka ọbịbịa calcium site na ọwa platelet TRPC6 [6].Echiche anyị bụ na glucose na-ebute izu ike nke microtubule tangles, na-eduga n'ịbawanye MPV na mmetụta platelet na/ma ọ bụ ịgbalite.Agbanyeghị, n'ịkpe ikpe site na nsonaazụ anyị, nke a bụ naanị akụkụ nke akụkọ ahụ.N'ime ule anyị, ọ nweghị ntinye uche dị n'okpuru D25W butere mmụba na MPV.Nyere na anyị anwalebeghị mkpughe nke mkpokọta glucose n'etiti 12.5% ​​na 25%, nsonaazụ anyị nke mbụ na-egosi na enwere ike ịnwe ọnụ ụzọ n'ụdị mkpokọta glucose a nke na-eduga na mmụba na MPV.Nnwale ndị ọzọ na nkebi 3 na 4 gosiri na glucose 20-25% yiri ka ọ bụ ọnụ ụzọ maka nke a, mana amabeghị ihe kpatara ya.
Anyị hụkwara mbelata ~9% na MPV mgbe centrifugation gasịrị.O dochaghị anya ma mbelata a na MPV bụ n'ihi platelet buru ibu ma dị njọ tọrọ n'ime oyi akwa RBC nke centrifuge.Nlebanya a nwere ike ịdị mkpa nye ndị dọkịta n'ihi na ọ nwere ike ịpụta na platelet PRP bụ obere platelet WB pere mpe ma pere mpe.
N'ime ọmụmụ ihe gara aga, anyị gosipụtara na nkwadebe PRP site na usoro ntuziaka adịghị ọnụ [8].Ọ bụrụ na glucose na-achọpụta platelet anụ ahụ ma ọ bụ PRP, na-eme ka ha dịkwuo mfe ịgbalite, ma ọ bụ ọ bụrụ na a na-emepụta PRP na akụkụ lysate akụkụ, nke a nwere ike ịkwalite mmụba ma belata mkpa ọgwụgwọ.Ya mere, nchikota nke PRP na glucose etinyere nke ukwuu nwere ike ịdị ọnụ ala karịa PRP ma ọ bụ glucose naanị.
Ọmụmụ ihe anyị nwere ọtụtụ adịghị ike.Nke mbụ, anyị na-eji PRP enwetara site na ụzọ dị iche iche.Nke a nwere ike ibute nsonaazụ na-emegiderịta onwe ya.Nke abụọ, anyị enweghị ike ịme nyocha biochemical nke ọ bụla n'ime ihe nlele anyị iji chọpụta nke ọma ma ịgbalite platelet mere.Ọ ga-amasị anyị ịlele P-selectin, platelet factor 4, monocytic platelet aggregates, ma ọ bụ ihe nrịbama ndị ọzọ nke ịgbalite platelet iji ghọta nke ọma ogo ma ọ bụ ọnụnọ nke mbibi alfa granule, mana nke a karịrị oke ọmụmụ a.Nke atọ, anyị enweghị ike ikwenye site na microscopy eletrọn ma ọ bụ ụzọ ndị ọzọ na mmụba nke MPV na platelet na-ekpughere glucose bụ n'ihi mmetụta na microtubule tangles.
Ngwakọta nke WB ma ọ bụ PRP nwere 25% glucose mụbara MPV, na-egosi mmalite mmalite nke platelet, n'agbanyeghị na ọmụmụ ihe a egosighi ọganihu nke nchịkọta ma ọ bụ mmebi.Ngwakọta glucose hypertonic butere mfu platelet, ikekwe na-anọchite anya mmetụta lytic.Ịgbalite obere akụkụ ma ọ bụ lysis nke platelet nwere ike ime ka anụ ahụ dịghachi ọhụrụ mgbe ịgba ọgwụ mgbochi platelet gasịrị.O dochaghị anya ihe nsonaazụ ụlọ ọgwụ mgbanwe ndị a nwere ike ibute.Ọmụmụ ihe ndị ọzọ egosila nha nke ịgbalite ma ọ bụ lysis ziri ezi ma enyochala nsonaazụ ụlọ ọgwụ dị iche iche nke ngwakọta glucose hypertonic na WB ma ọ bụ PRP.
Usoro ọgwụgwọ glucose proliferative ọgwụgwọ bụ ọgwụgwọ mmeghari dị mfe ma dị ọnụ ala nke na-agbasa ngwa ngwa ma na-akwado nyocha ụlọ ọgwụ.Ọmụmụ ihe a na-egosi usoro ihe ọmụmụ physiological nke, ọ bụrụ na akwadoro ya, nwere ike inyere anyị aka ịghọta akụkụ nke usoro mmeghari nke ọgwụgwọ proliferative.
Biomedical na Health Informatics na Mahadum Missouri, Kansas City School of Medicine, Kansas City, USA
Ihe gbasara mmadụ: Ndị niile sonyere na ọmụmụ ihe a nyere ma ọ bụ enyeghị nkwenye.International Society for Cellular Medicine enyela nkwado ICMS-2017-003.Ndị ụlọ ọrụ nyocha ụlọ ọrụ International Society for Cellular Medicine ka akwadoro ụkpụrụ ndị a maka ojiji ọzọ: Isiokwu: Ngụkọta mkpụrụ ọgwụ plasma bara ụba nke platelet dabere na ntọala CBC platelet.Ihe gbasara anụmanụ: Ndị ode akwụkwọ niile kwadoro na ọ nweghị anụmanụ ma ọ bụ anụ ahụ etinyere aka na ọmụmụ a.Esemokwu nke Mmasị: N'ikwekọ n'ụdị ngosi ICMJE Uniform, ndị edemede niile na-ekwupụta ihe ndị a: Ozi ịkwụ ụgwọ/ozi ọrụ: Ndị ode akwụkwọ niile na-ekwupụta na ha enwetaghị nkwado ego n'aka ụlọ ọrụ ọ bụla maka ọrụ etinyere.Mmekọrịta ego: Ndị ode akwụkwọ niile na-ekwupụta na ha enweghị ugbu a ma ọ bụ n'ime afọ atọ gara aga nwere mmekọrịta ego na ụlọ ọrụ ọ bụla nwere ike ịmasị ọrụ etinyere.Mmekọrịta ndị ọzọ: Ndị ode akwụkwọ niile na-ekwupụta na enweghị mmekọrịta ma ọ bụ mmemme ndị ọzọ nwere ike imetụta ọrụ etinyere.
Harrison TE, Bowler J, Reeves K et al.(Mee 17, 2022) Mmetụta nke glucose na ọnụ ọgụgụ platelet na olu: ihe ọ pụtara maka ọgwụ na-emegharị ahụ.Ọgwụgwọ 14 (5): e25081.doi:10.7759/cureus.25081
© Copyright 2022 Harrison et al.Nke a bụ akụkọ ohere mepere emepe ekesara n'okpuru usoro ikike nke Creative Commons Attribution CC-BY 4.0.Eji, nkesa na mmeputakwa na-akparaghị ókè anabatara na usoro ọ bụla, ma ọ bụrụhaala na akwadoro onye edemede mbụ na isi mmalite.