Emepụtala igwekota static inline ọhụrụ na-agbanwe agbanwe nke ọma iji gboo mkpa siri ike nke sistemu chromatography mmiri mmiri (HPLC) yana ultra high performance liquid chromatography (HPLC na UHPLC).Ngwakọta na-adịghị mma nke usoro mkpanaka abụọ ma ọ bụ karịa nwere ike ibute oke mgbama na mkpọtụ dị elu, nke na-ebelata mmetụta.Ngwakọta static dị n'otu nke mmiri abụọ ma ọ bụ karịa nwere opekempe olu dị n'ime yana akụkụ anụ ahụ nke igwekota static na-anọchi anya ọkọlọtọ kachasị elu nke ezigbo igwekota static.Ngwakọta static ọhụrụ a na-enweta nke a site na iji teknụzụ obibi akwụkwọ 3D ọhụrụ iji mepụta usoro 3D pụrụ iche nke na-enye ngwakọta static hydrodynamic ka mma yana mbelata pasentị kachasị elu na isi sine wave kwa nkeji n'ime olu nke ngwakọta.Iji 1/3 nke olu dị n'ime nke igwe na-agwakọta ihe na-ebelata isi ebili mmiri site na 98%.Ngwakọta ahụ nwere ọwa mmiri 3D jikọtara ọnụ yana mpaghara akụkụ dị iche iche na ogologo ụzọ ka mmiri ahụ na-agafe geometries 3D gbagwojuru anya.Ịgwakọta n'ọtụtụ ụzọ na-asọ asọ, jikọtara ya na ọgba aghara mpaghara na eddies, na-ebute ịgwakọta na micro, meso na nnukwu akpịrịkpa.Emebere igwekota a pụrụ iche site na iji simulations computational fluid dynamics (CFD).Data ule ewepụtara na-egosi na a na-enweta ngwakọta dị mma site na obere olu dị n'ime.
Kemgbe ihe karịrị afọ 30, ejirila chromatography mmiri mmiri mee ihe n'ọtụtụ ụlọ ọrụ, gụnyere ọgwụ, ọgwụ pesticides, nchekwa gburugburu ebe obibi, nyocha nyocha, na nyocha kemịkalụ.Ikike iji tụọ akụkụ kwa nde ma ọ bụ obere dị oke mkpa maka mmepe teknụzụ na ụlọ ọrụ ọ bụla.Ịgwakọta ọnụ na-adịghị mma na-eduga n'ókè mgbaàmà na-adịghị mma, nke bụ mkpasu iwe nye obodo chromatography n'ihe gbasara oke nchọpụta na nghọta.Mgbe ị na-agwakọta ihe mgbaze HPLC abụọ, ọ na-adị mkpa mgbe ụfọdụ ịmanye ịgwakọta site na mpụga n'ụzọ iji homogenize ihe mgbaze abụọ ahụ n'ihi na ụfọdụ ihe mgbaze adịghị agwakọta nke ọma.Ọ bụrụ na agwakọtaghị ihe mgbaze nke ọma, nbibi nke chromatogram HPLC nwere ike ime, na-egosipụta onwe ya dị ka oke mkpọtụ ntọala na/ma ọ bụ ọdịdị elu adịghị mma.Site na ngwakọta na-adịghị mma, ụda ntọala ga-apụta dị ka ebili mmiri sine (na-ebili ma na-ada) nke ihe nrịbama n'ime oge.N'otu oge ahụ, ịgwakọta adịghị mma nwere ike iduga n'ịgbasawanye na ọnụ ọgụgụ asymmetric, na-ebelata arụmọrụ nyocha, ọdịdị kachasị elu, na mkpebi kachasị elu.Ụlọ ọrụ ahụ achọpụtala na in-line na tee static mixers bụ ụzọ isi melite oke ndị a na ikwe ka ndị ọrụ nweta njedebe nchọpụta dị ala (sensitivities).Ngwakọta igwe kwụ ọtọ nke ọma na-ejikọta uru nke arụmọrụ ngwakọta dị elu, obere olu nwụrụ anwụ na obere nrụgide na-adaba na olu kacha nta na ntinye usoro kachasị.Na mgbakwunye, ka nyocha na-adịwanye mgbagwoju anya, ndị nyocha ga-ejikarị polar na ihe mgbaze siri ike na-agwakọta.Nke a pụtara ịgwakọta ka mma bụ ihe dị mkpa maka nnwale n'ọdịnihu, na-abawanye mkpa maka imewe igwekota dị elu na arụmọrụ.
N'oge na-adịbeghị anya Mott ewepụtala ụdị patent nke PerfectPeakTM inline static mixers nwere mpịakọta dị n'ime atọ: 30 µl, 60 µl na 90 µl.Nha ndị a na-ekpuchi oke mpịakọta na njirimara agwakọta dị mkpa maka ọtụtụ ule HPLC ebe achọrọ ka agwakọta ka mma na mgbasa ozi dị ala.Ụdị atọ ahụ bụ 0.5 ″ na dayameta ma na-ebute arụmọrụ na-eduga ụlọ ọrụ na nhazi kọmpat.A na-eme ha nke igwe anaghị agba nchara 316L, nke na-agafe maka inertness, mana titanium na ihe ndị ọzọ na-eguzogide corrosion na kemịkal inert metal alloys dịkwa.Ngwakọta ndị a nwere oke ọrụ arụ ọrụ ruru 20,000 psi.Na fig.1a bụ foto nke igwekota static 60 µl Mott emebere iji wepụta arụmọrụ kacha mma ka ị na-eji obere olu dị n'ime karịa ndị na-agwakọta ọkọlọtọ nke ụdị a.Nhazi igwekota static ọhụrụ a na-eji teknụzụ nrụpụta ihe mgbakwunye ọhụrụ iji mepụta usoro 3D pụrụ iche nke na-eji obere ihe n'ime ime karịa igwe igwe ọ bụla ejiri ugbu a na ụlọ ọrụ chromatography iji nweta ngwakọta static.Ngwakọta ndị dị otú ahụ nwere ọwa mmiri na-asọ asọ nke nwere akụkụ atọ nwere njikọ dị iche iche na ogologo ụzọ dị iche iche ka mmiri mmiri na-agafe ihe mgbochi geometric dị mgbagwoju anya n'ime.Na fig.Onyonyo 1b na-egosi eserese schematic nke igwekota ọhụrụ, nke na-eji ọkọlọtọ ụlọ ọrụ 10-32 threaded HPLC mkpakọ maka ntinye na ntinye, ma kpuchie oke anụnụ anụnụ nke ọdụ ụgbọ mmiri mixer.Ebe dị iche iche na-agafe agafe nke ụzọ ntinye n'ime ime na mgbanwe na ntụgharị ntụgharị n'ime ụda olu na-emepụta mpaghara nke mgbagwoju anya na laminar, na-eme ka ngwakọta na micro, meso na macro scales.Nhazi nke igwekota a pụrụ iche jiri simulations computational fluid dynamics (CFD) nyochaa usoro eruba ma mezie nhazi ahụ tupu prototyping maka ule nyocha n'ime ụlọ yana nyocha mpaghara ndị ahịa.Nrụpụta mgbakwunye bụ usoro nke ibipụta ngwa geometric 3D ozugbo site na eserese CAD na-enweghị mkpa maka igwe ọdịnala (igwe igwe igwe, lathes, wdg).A na-emepụta mixers ọhụrụ ndị a ka a na-emepụta site na iji usoro a, ebe a na-emepụta ahụ igwekota site na eserese CAD na akụkụ ndị ahụ na-emepụta oyi akwa (bipụta) site na oyi akwa site na iji mmepụta ihe.N'ebe a, a na-edobe oyi akwa ntụ ntụ nke dị ihe dị ka micron 20, na laser kọmputa na-achị ahọrọ na-agbaze ma gbanye ntụ ntụ ahụ n'ụdị siri ike.Tinye akwa oyi akwa ọzọ n'elu oyi akwa a ma tinye singing laser.Tinyegharịa usoro a ruo mgbe akụkụ ahụ gwụchara.A na-ewepụ ntụ ntụ ahụ na akụkụ nke na-abụghị laser, na-ahapụ akụkụ 3D ebipụtara dabara na ihe osise CAD mbụ.Ngwaahịa ikpeazụ dịtụ ka usoro microfluidic, yana ihe dị iche bụ na akụkụ microfluidic na-abụkarị akụkụ abụọ (flat), ebe ị na-eji nrụpụta mgbakwunye, enwere ike ịmepụta usoro ịgba ọsọ dị mgbagwoju anya na geometry akụkụ atọ.Igwe mmiri ndị a dị ugbu a dị ka akụkụ 3D ebipụtara na 316L igwe anaghị agba nchara na titanium.Enwere ike iji ọtụtụ alloys metal, polymers na ụfọdụ ceramik mee ihe site na iji usoro a, a ga-atụlekwa ya na nhazi/ ngwaahịa n'ọdịnihu.
Osikapa.1. Foto (a) na eserese (b) nke igwekota 90 μl Mott static mixer na-egosi akụkụ obe nke ụzọ mmiri mmiri igwekota agwakọta na-acha anụnụ anụnụ.
Gbaa simulations computational fluid dynamics (CFD) nke arụmọrụ igwekota static n'oge usoro nhazi iji nyere aka mepụta atụmatụ dị mma ma belata nnwale nnwale na njehie na-eri oge yana ọnụ.CFD simulation nke static mixers na ọkọlọtọ ọkpọkọ (no-mixer simulation) na-eji COMSOL Multiphysics software ngwugwu.Nlegharị anya site na iji igwe igwe mmiri laminar na-ebugharị iji ghọta ọsọ mmiri na nrụgide n'ime akụkụ.Mgbanwe nke mmiri a, jikọtara ya na njem kemịkalụ nke ogige a na-ebugharị mkpanaka, na-enyere aka ịghọta ngwakọta nke mmiri mmiri abụọ dị iche iche.A na-amụ ihe nlereanya ahụ dị ka ọrụ nke oge, hà nhata 10 sekọnd, maka ịdị mfe nke ngụkọta oge mgbe ị na-achọ ngwọta ndị yiri ya.Enwetara data usoro ihe omume na ọmụmụ ihe jikọtara oge site na iji ngwa nyocha ihe nyocha, ebe a na-ahọrọ ebe dị n'etiti ụzọ ọpụpụ maka nchịkọta data.Ụdị CFD na nnwale nnwale na-eji ihe mgbaze abụọ dị iche iche site na valvụ nlele nha nha na sistemụ mgbapụta, na-ebute nkwụnye ngbanwe maka ihe mgbaze ọ bụla na ahịrị nlele.A na-agwakọta ihe mgbaze ndị a na igwekota static.Ọnụọgụ 2 na 3 na-egosi simulations eruba site na ọkpọkọ ọkọlọtọ (enweghị igwekota) yana site na igwekota Mott static, n'otu n'otu.A na-eme ihe ngosi ahụ na tube kwụ ọtọ 5 cm n'ogologo na 0.25 mm ID iji gosipụta echiche nke mgbanwe plọg mmiri na acetonitrile dị ọcha n'ime tube na-enweghị ihe mgbagwoju anya, dị ka e gosiri na Figure 2. Ihe ngosi na-eji kpọmkwem akụkụ nke tube na mixer na ọnụ ọgụgụ na-asọpụta nke 0.3 ml / min.
Osikapa.2. Simulation nke CFD eruba na a 5 cm tube na esịtidem dayameta nke 0.25 mm na-anọchi anya ihe na-eme na HPLC tube, ie na-anọghị nke igwekota.Uhie zuru oke na-anọchite anya oke mmiri.Blue na-anọchi anya enweghị mmiri, ntụgharị acetonitrile dị ọcha.Enwere ike ịhụ mpaghara mgbasa ozi n'etiti plọgụ ndị ọzọ nke mmiri mmiri abụọ dị iche iche.
Osikapa.3. Ngwakọta static nwere olu nke 30 ml, nke emere na ngwugwu ngwanrọ COMSOL CFD.Akụkọ a na-egosi oke mmiri dị na igwekota.A na-egosi mmiri dị ọcha na ọbara ọbara yana acetonitrile dị ọcha na-acha anụnụ anụnụ.Mgbanwe nke oke nha nke mmiri simulated na-anọchi anya mgbanwe agba nke ngwakọta nke mmiri mmiri abụọ.
Na fig.4 na-egosi nnwapụta nkwado nke ụdị mmekọrịta dị n'etiti ịgwakọta arụmọrụ na ịgwakọta olu.Ka ụda ngwakọta na-abawanye, arụmọrụ ngwakọta ga-abawanye.N'ihe ndị odee mara, ike anụ ahụ ndị ọzọ dị mgbagwoju anya na-arụ ọrụ n'ime igwekota enweghị ike ịgụta ya na ụdị CFD a, na-ebute arụmọrụ ngwakọta dị elu na ule nnwale.A tụrụ arụmọrụ ngwakọta nnwale ahụ dị ka mbelata pasentị na sinusoid isi.Na mgbakwunye, mmụba azụ azụ na-ebutekarị ọkwa ngwakọta dị elu, nke a na-ejighị n'aka na simulation.
A na-eji ọnọdụ HPLC ndị a na ntọlite ​​ule iji tụọ ebili mmiri raw iji tụnyere arụmọrụ nke ndị mixers static dị iche iche.Eserese dị na eserese 5 na-egosi nhazi usoro HPLC/UHPLC.A nwalere igwekota static site n'itinye igwekota ozugbo mgbe mgbapụta ahụ na tupu injector na kọlụm nkewa.A na-eme ọtụtụ nha sinusoidal ndabere site na ịgafe kọlụm injector na capillary n'etiti igwekota static na ihe nchọpụta UV.Mgbe ị na-enyocha ogo mgbama-na-mkpọtụ na/ma ọ bụ na-enyocha udi elu, a na-egosi nhazi usoro na eserese 5.
Onyonyo 4. Atụmatụ agwakọta arụmọrụ megide agwakọta olu maka a nso nke static mixers.Ihe adịghị ọcha nke usoro ihe ọmụma na-agbaso otu usoro ahụ dị ka data adịghị ọcha nnwale na-akwado izi ezi nke simulations CFD.
Sistemu HPLC ejiri mee nnwale a bụ Agilent 1100 Series HPLC nwere ihe nchọta UV nke PC na-agba ngwa Chemstation na-achịkwa.Tebụl 1 na-egosi ọnọdụ nlegharị anya na-ahụkarị maka ịlele arụmọrụ igwekota site na nyochaa isi sinusoids na ọmụmụ ihe abụọ.Emere ule nnwale na ihe atụ abụọ dị iche iche nke ihe mgbaze.Ihe mgbaze abụọ a gwakọtara ma ọ bụrụ na 1 bụ ihe mgbaze A (20 mM ammonium acetate na mmiri deionized) na ihe mgbaze B (80% acetonitrile (ACN) / 20% mmiri deionized).N'okwu nke 2, ihe mgbaze A bụ ihe ngwọta nke 0.05% acetone (label) na mmiri deionized.Ihe mgbaze B bụ ngwakọta nke 80/20% methanol na mmiri.N'ihe banyere 1, a na-etinye mgbapụta ahụ ka ọ bụrụ 0.25 ml / min na 1.0 ml / min, na n'ọnọdụ 2, a na-etinye mgbapụta ahụ ka ọ bụrụ 1 ml / min.N'okwu abụọ ahụ, oke nke ngwakọta nke ihe mgbaze A na B bụ 20% A / 80% B. A na-edozi ihe nchọpụta ahụ na 220 nm ma ọ bụrụ na 1, na ntinye kachasị nke acetone ma ọ bụrụ na 2 ka edobere n'ogologo nke 265 nm.
Tebụl 1. Nhazi HPLC maka ikpe 1 na 2 Case 1 Case 2 Pump Speed ​​​​0.25 ml/min ka 1.0 ml/min 1.0 ml/min Ihe mgbaze A 20 mM ammonium acetate na mmiri deionized 0.05% Acetone na deionized mmiri mgbaze B 80% ACNenetoi 2% / 0.0% nke mmiri na-edozi mmiri. Ihe mgbaze mmiri deionized 20% A / 80% B 20% A / 80% B Nchọpụta 220 nm 265 nm
Osikapa.6. Ntube nke ebili mmiri sine agwakọtara tụrụ tupu na mgbe etinyechara nzacha dị ala iji wepụ ihe ndị na-ebugharị ihe mgbama.
Onyonyo 6 bụ ihe atụ atụ nke ụda ntọala agwakọtara agwakọta na Ikpe 1, nke egosiri dị ka usoro sinusoidal na-emegharị ugboro ugboro na-atụkwasịrị na mbugharị ntọala.Mfefe ndabere bụ mmụba nwayọ ma ọ bụ mbelata na mgbama azụ.Ọ bụrụ na ekweghị ka usoro ahụ guzozie ogologo oge, ọ ga-adabakarị, mana ọ ga-agafe n'ụzọ na-ezighị ezi ọbụna mgbe usoro ahụ kwụsiri ike.Ntugharị ntọala a na-ebuwanye ibu mgbe sistemụ na-arụ ọrụ na ọnọdụ mkpọda ugwu ma ọ bụ ọnọdụ nrụgide azụ dị elu.Mgbe mgbagharị ntọala a dị, ọ nwere ike isi ike iji tụnyere nsonaazụ site na nlele gaa na nlele, nke enwere ike imeri site na itinye nzacha dị ala na data raw iji yochaa mgbanwe ndị a dị ala, si otú ahụ na-enye nkata oscillation na ntọala dị larịị.Na fig.Onyonyo 6 na-egosikwa nkata nke mkpọtụ ntọala igwekota mgbe etinyechara nzacha dị ala.
Mgbe emechara ihe ngosi CFD na nnwale nnwale izizi, e mechara mepụta igwekota static atọ dị iche iche site na iji ihe dị n'ime akọwara n'elu nwere mpịakọta dị n'ime atọ: 30 µl, 60 µl na 90 µl.Nke a nso na-ekpuchi nso nke mpịakọta na Ịgwakọta arụmọrụ chọrọ ala analyte HPLC ngwa ebe mma Ịgwakọta na ala dispersion na-chọrọ iji mepụta ala amplitude baselines.Na fig.7 na-egosi nha nha ebili mmiri bụ isi enwetara na sistemụ nnwale nke Ihe Nlereanya 1 (acetonitrile na ammonium acetate dị ka tracers) nwere mpịakọta atọ nke igwekota static na enweghị igwekota.Ọnọdụ nnwale nnwale maka nsonaazụ egosiri na eserese 7 ka a na-eme mgbe niile n'ime ule 4 niile dịka usoro akọwapụtara na Tebụl 1 na ọnụego mgbaze nke 0.5 ml / min.Tinye uru nkwụghachi na dataset ka enwere ike igosipụta ha n'akụkụ n'akụkụ na-enweghị mgbakwụnye mgbaama.Nkwụsị adịghị emetụta njupụta nke mgbama ejiri mee ka ọ bụrụ ọkwa arụmọrụ nke igwekota.Nkezi njupụta sinusoidal na-enweghị igwekota bụ 0.221 mAi, ebe njupụta nke static Mott mixers na 30 µl, 60 µl, na 90 µl gbadara ruo 0.077, 0.017, na 0.004 mAi, n'otu n'otu.
Ọgụgụ 7. HPLC UV Detector Signal Offset vs. Oge maka ikpe 1 (acetonitrile na ammonium acetate egosi) na-egosi ngwakọta ihe mgbaze na-enweghị igwekota, 30 µl, 60 µl na 90 µl Mott mixers na-egosi ngwakọta ka mma (njupụta mgbama ala ala) dị ka olu nke static mixer na-abawanye.(Nkwụsị data n'ezie: 0.13 (enweghị igwekota), 0.32, 0.4, 0.45mA maka ngosi ka mma).
Data egosiri na fig.8 bụ otu ihe ahụ dị na Fig. 7, mana oge a ha na-agụnye nsonaazụ HPLC static mixers atọ a na-ejikarị nwere mpịakọta ime nke 50 µl, 150 µl na 250 µl.Osikapa.Ọgụgụ 8. HPLC UV Detector Signal Offset versus Time Plot for Case 1 (Acetonitrile na Ammonium Acetate dị ka egosi) na-egosi ngwakọta nke ihe mgbaze na-enweghị static mixer, ọhụrụ usoro nke Mott static mixers, na atọ omenala mixers (n'ezie data offset bụ 0.1 (enweghị igwekota), 0.480, 0.0.7 m, 0.48, 0.32, 8. A otu maka mma ngosi mmetụta).A na-agbakọ mbelata pasentị nke ebili mmiri ntọala site na nha nke njupụta nke ebili mmiri sine na njupụta na-enweghị igwekota.Pasent attenuation sine ebili mmiri tụrụ atụ maka ikpe 1 na 2 ka edepụtara na Tebụlụ 2, yana mpịakọta dị n'ime nke igwekota static ọhụrụ yana igwe agwakọta ọkọlọtọ asaa a na-ejikarị na ụlọ ọrụ.Ihe omuma a di na onu ogugu 8 na 9, yana ngụkọ ewepụtara na Tebụl 2, na-egosi na Mott Static Mixer nwere ike inye ihe ruru 98.1% attenuation sine, karịa arụmọrụ nke igwekota HPLC nkịtị n'okpuru ọnọdụ ule ndị a.Ọnụọgụ 9. HPLC UV detector nkwụghachi ụgwọ n'usoro oge maka ikpe 2 (methanol na acetone dị ka tracers) na-egosi na ọ dịghị static mixer (jikọtara), usoro ọhụrụ nke Mott static mixers na abụọ mixers (n'ezie data offsets bụ 0, 11 (na-enweghị mixer.), 0.22, 0.3, 0.35 maka ngosi ka mma).A tụlekwara igwekota asaa a na-ejikarị na ụlọ ọrụ ahụ.Ndị a na-agụnye mixers nwere mpịakọta dị n'ime atọ dị iche iche sitere na ụlọ ọrụ A (nke a họpụtara Mixer A1, A2 na A3) na ụlọ ọrụ B (nke a họpụtara Mixer B1, B2 na B3).Ụlọ ọrụ C nyere naanị otu nha.
Tebụl 2. Ngwakọta agwakọta Static Static Characterities and Internal Volume Static Mixer Case 1 Sinusoidal Iweghachite: Acetonitrile Test (Irụ Ọrụ) Ikpe 2 Sinusoidal Iweghachite: Methanol Water Test (Erụmọrụ) Mpịakọta Ime (µl) Ọ dịghị Ngwakọta - - 0 Mott 30 62.6% 0.3% 60 Mott 90 98.1% 97.5% 90 igwekota A1 66.4% 73.7% 50 igwekota A2 89.8% 91.6% 150 igwekota A3 92.2% 94.5% 250 igwekota B1 44.8% 9338 mixer B1 44.8% 45.75. Ngwakọta C 97.2% 97.4% 250
Nyocha nke nsonaazụ dị na eserese 8 na tebụl 2 na-egosi na 30 µl Mott static mixer nwere otu arụmọrụ ngwakọta dị ka igwekota A1, ya bụ 50 µl, agbanyeghị, 30 µl Mott nwere 30% obere olu dị n'ime.Mgbe atụnyere 60 µl Mott mixer na 150 µl n'ime olu A2 igwekota, enwere ntakịrị mmụba n'ịgwakọta arụmọrụ nke 92% megide 89%, mana nke ka mkpa, enwetara ọkwa dị elu nke ngwakọta a na 1/3 nke olu igwekota.yiri igwekota A2.Arụmọrụ nke igwekota 90 µl Mott gbasoro otu omume ahụ dị ka igwekota A3 nwere oke ime nke 250 µl.A hụkwara mmụba n'ịgwakọta arụmọrụ nke 98% na 92% site na mbelata okpukpu atọ n'ime olu dị n'ime.E nwetara nsonaazụ na ntụnyere yiri nke ahụ maka mixers B na C. N'ihi ya, usoro ọhụrụ nke static mixers Mott PerfectPeak TM na-enye arụmọrụ dị elu nke mixing karịa ndị na-asọmpi asọmpi, ma na obere olu dị n'ime, na-enye ụda ụda azụ ka mma na akara ngosi ka mma nke ọma, nyocha nke ọma, ọdịdị elu na mkpebi kacha elu.Ahụrụ usoro ndị yiri nke ahụ na ngwakọta nke ọma n'ihe ọmụmụ nke mbụ na nke abụọ.Maka ikpe 2, a na-eme ule site na iji (methanol na acetone dị ka ihe ngosi) iji tụnyere ịdị mma nke 60 ml Mott, igwekota A1 (olu dị n'ime 50 µl) na mixer yiri B1 (oke ime 35 µl)., arụmọrụ adịghị mma na-enweghị onye na-agwakọta ihe, ma a na-eji ya mee ihe maka nyocha ntọala.Ngwakọta Mott 60 ml gosipụtara na ọ bụ igwekota kacha mma n'ime otu nnwale ahụ, na-enye mmụba 90% na nrụpụta ngwakọta.Ngwakọta Ngwakọta atụnyere A1 hụrụ mmụba 75% n'ịgwakọta arụmọrụ yana mmụba 45% na igwekota B1 yiri ya.A na-eme ule mbelata sine ebili mmiri bụ isi na ọnụego nrịba na usoro igwekota n'okpuru otu ọnọdụ dị ka ule sine curve dị na Case 1, na naanị ọnụego mgbanwe gbanwere.Ihe omuma a gosiputara na n'ogo nke nfe site na 0.25 ruo 1 ml/min, mbelata nke mbu na ebili mmiri sine nọgidere na-adịgide adịgide maka mpịakọta igwekota atọ niile.Maka ndị na-agwakọta ụda olu abụọ dị ntakịrị, a na-enwe ntakịrị mmụba na nkwụsị nke sinusoidal ka ọnụ ọgụgụ na-agbapụta na-ebelata, nke a na-atụ anya n'ihi ụbara oge obibi nke ihe mgbaze na mixer, na-enye ohere maka ịba ụba mgbasa ozi.A na-atụ anya na mwepu nke ebili mmiri sine ga-abawanye ka mmiri na-agbada n'ihu.Otú ọ dị, maka nnukwu igwekota olu nwere oke sine wave base attenuation, attenuation sine wave base attenuation nọgidere na-agbanweghị agbanwe (n'ime oke nnwale nnwale), yana ụkpụrụ sitere na 95% ruo 98%.Osikapa.10. Attenuation bụ isi nke sine wave versus na-agba ọsọ na ikpe 1. A na-eme nyocha ahụ n'okpuru ọnọdụ ndị yiri ule sine na ọnụego mgbanwe mgbanwe, na-agbanye 80% nke ngwakọta 80/20 nke acetonitrile na mmiri na 20% nke 20 mM ammonium acetate.
The ọhụrụ emepụtara patented PerfectPeakTM inline static mixers nwere atọ dị n'ime mpịakọta: 30 µl, 60 µl na 90 µl na-ekpuchi olu na agwakọta arụmọrụ nso chọrọ maka ọtụtụ HPLC analysis na-achọ mma ịgwakọta na ala mgbasa ozi ala.Ngwakọta igwe kwụ otu ebe ọhụrụ na-enweta nke a site na iji teknụzụ obibi akwụkwọ 3D ọhụrụ iji mepụta usoro 3D pụrụ iche nke na-enye ngwakọta static hydrodynamic ka mma yana mbelata pasentị kachasị elu na mkpọtụ ntọala kwa otu olu nke ngwakọta ime.Iji 1/3 nke olu dị n'ime nke igwe na-agwakọta ihe na-ebelata ụda isi site na 98%.Ngwakọta ndị dị otú ahụ nwere ọwa mmiri na-asọ asọ nke nwere akụkụ atọ nwere njikọ dị iche iche na ogologo ụzọ dị iche iche ka mmiri mmiri na-agafe ihe mgbochi geometric dị mgbagwoju anya n'ime.Ezinụlọ ọhụrụ nke ndị mixers static na-enye arụmọrụ ka mma karịa ndị na-agwakọta asọmpi, mana na-enwe obere olu dị n'ime, na-ebute oke mgbaàmà na mkpọtụ dị mma yana oke ọnụọgụ dị ala, yana nkwalite ọdịdị elu, arụmọrụ na mkpebi maka inwe mmetụta dị elu.
Na mbipụta a Chromatography - RP-HPLC na gburugburu ebe obibi - Iji chromatography isi-shell dochie acetonitrile na isopropanol na nyocha na ịdị ọcha - chromatograph gas ọhụrụ maka…
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