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Ibidukikije byita ku buzima byanduye bigira uruhare runini mu gukwirakwiza ibinyabuzima birwanya imiti myinshi (MDR) na C. diffile.Icyari kigamijwe muri ubu bushakashatsi kwari ugusuzuma ingaruka za ozone ikorwa na reakteri ya plasma ya dielectric (DBD) ya plasma ku gikorwa cya vancomycine irwanya Enterococcus faecalis (VRE), karbapenem irwanya Klebsiella pneumoniae (CRE), karbapenem-irwanya Antibacterial yibikoresho byanduye na Pseudomas.Pseudomonas aeruginosa (CRPA), karbapenem irwanya Acinetobacter baumannii (CRAB) na Clostridium difficile spores.Ibikoresho bitandukanye byandujwe na VRE, CRE, CRPA, CRAB na C. spifile spicile yavuwe hamwe na ozone mugihe cyo kwibanda hamwe nibihe bitandukanye.Imbaraga za atome microscopi (AFM) yerekanye ihinduka rya bagiteri nyuma yo kuvura ozone.Iyo ikinini cya 500 ppm ozone cyashyizwe kuri VRE na CRAB mu minota 15, igabanuka rya logi hafi 2 cyangwa irenga ryagaragaye mu byuma bitagira umwanda, imyenda n'ibiti, kandi kugabanuka kwa log10 1-2 byagaragaye mu kirahure na plastiki.C. sporile spifile wasangaga irwanya ozone kurusha ibindi binyabuzima byose byapimwe.Kuri AFM, nyuma yo kuvurwa na ozone, bagiteri zabyimbye kandi zirahinduka.Ozone yakozwe na DBD Plasma Reactor nigikoresho cyoroshye kandi gifite agaciro ko kwanduza MDRO na C. diffile spores, izwiho kuba ari indwara zanduza indwara ziterwa n'ubuzima.
Kuba havutse ibinyabuzima birwanya imiti myinshi (MDR) biterwa no gukoresha nabi antibiotike mu bantu no ku nyamaswa kandi byagaragaye ko Umuryango w’ubuzima ku isi (OMS) ari ikibazo gikomeye ku buzima rusange1.By'umwihariko, ibigo nderabuzima bigenda bihura n’ikwirakwizwa rya MRO.MRO nyamukuru ni methicilline irwanya Staphylococcus aureus na enterococcus irwanya vancomycine (VRE), yagutse-ya beta-lactamase itanga enterobacteria (ESBL), irwanya imiti myinshi ya Pseudomonas aeruginosa, irwanya Acinobacter baumannii, na Carbapenemi.Byongeye kandi, indwara ya Clostridium difficile nimwe mu mpamvu zitera impiswi ziterwa n'ubuzima, zishyira umutwaro ukomeye kuri gahunda z'ubuzima.MDRO na C. diffile yanduzwa binyuze mu biganza by'abakozi bashinzwe ubuzima, ibidukikije byanduye, cyangwa biturutse ku muntu ku muntu.Ubushakashatsi buherutse gukorwa bwerekanye ko ibidukikije byanduye mu rwego rw’ubuzima bigira uruhare runini mu kwanduza MDRO na C. diffile igihe abakozi b’ubuzima (HCWs) bahuye n’imiterere yanduye cyangwa iyo abarwayi bahuye n’ubutaka bwanduye 3,4.ibidukikije byanduye mubuzima bwubuzima bigabanya kwandura MLRO na C. kwandura cyangwa gukolonizwa 5,6,7.Urebye impungenge ku isi hose ziyongera ku kurwanya imiti igabanya ubukana bwa virusi, biragaragara ko hakenewe ubushakashatsi bwinshi ku buryo n’uburyo bwo kwanduza indwara z’ubuzima.Vuba aha, uburyo bwo gusukura itumanaho, cyane cyane ibikoresho bya ultraviolet (UV) cyangwa sisitemu ya hydrogen peroxide, byamenyekanye nkuburyo butanga uburyo bwo kwanduza.Nyamara, ibyo bikoresho biboneka mubucuruzi UV cyangwa hydrogen peroxide ntabwo bihenze gusa, kwanduza UV bigira akamaro gusa hejuru yubutaka bugaragara, mugihe hydrogène peroxide plasma yanduza bisaba igihe kirekire cyo kwanduza mbere yikizunguruka gikurikira5.
Ozone izwiho kurwanya ruswa kandi irashobora kubyazwa umusaruro8.Birazwi kandi ko ari uburozi kubuzima bwabantu, ariko birashobora kubora vuba muri ogisijeni 8. Imiyoboro ya plasma ya dielectric (DBD) yamashanyarazi niyo itanga ingufu za ozone cyane9.Ibikoresho bya DBD bigufasha gukora plasma yubushyuhe buke mukirere no kubyara ozone.Kugeza ubu, ikoreshwa rya ozone ryagarukiye gusa ku kwanduza amazi yo koga, amazi yo kunywa n’imyanda10.Ubushakashatsi bwinshi bwerekanye ko bukoreshwa mu buvuzi8,11.
Muri ubu bushakashatsi, twifashishije imashini itanga amashanyarazi ya DBD plasma ozone kugira ngo tugaragaze akamaro kayo mu gukuraho MDRO na C. diffile, ndetse n’ibiterwa ku bikoresho bitandukanye bikunze gukoreshwa mu buvuzi.Byongeye kandi, uburyo bwa ozone bwo kuboneza urubyaro bwasobanuwe hakoreshejwe microscopi ya atomic force (AFM) ya selile zivuwe na ozone.
Imbaraga zabonetse mu bwigunge bwa:bagiteri Pseudomonas aeruginosa (CRPA; 54 na 83).irwanya Acinetobacter baumannii (CRAB; F2487 na SCH-511).C. difficile yabonetse mu cyegeranyo cy’umuco w’indwara ya Patogene (NCCP 11840) y’ikigo cya Koreya gishinzwe kurwanya no gukumira indwara.Yatandukanijwe n’umurwayi wo muri Koreya yepfo muri 2019 ugasanga ari iya ST15 ukoresheje imashini ikurikirana.Umutima Wubwonko (BHI) Umuyoboro (BD, Sparks, MD, USA) watewe na VRE, CRE, CRPA na CRAB wavanze neza kandi ushyirwa kuri 37 ° C. mumasaha 24.
C. diffile yashizwemo anaerobically kumaraso agar kumasaha 48.Abakoloni benshi noneho bongerewe kuri ml 5 yumunyu wumutima wubwonko hanyuma ushyirwa mubihe bya anaerobic mumasaha 48.Nyuma yibyo, umuco warahungabanye, ml 5 ya 95% ya Ethanol yongeweho, irongera iranyeganyezwa hanyuma isigara ku bushyuhe bwicyumba muminota 30.Nyuma ya centrifugation kuri 3000 g muminota 20, fata ndengakamere hanyuma uhagarike pellet irimo spore hanyuma wice bagiteri muri ml 0.3 y'amazi.Ingirabuzimafatizo zifatika zabaruwe no gutera imbuto ya bagiteri ihagarikwa kumasahani yamaraso nyuma yo kuyungurura neza.Ikibonezamvugo cyemeje ko 85% kugeza 90% byimiterere ya bagiteri ari spore.
Ubushakashatsi bukurikira bwakozwe kugira ngo hakorwe ubushakashatsi ku ngaruka za ozone nka disinfectant ku bice bitandukanye byandujwe na MDRO na C. diffile spores, izwiho gutera indwara ziterwa n'ubuzima.Tegura ingero z'ibyuma bitagira umwanda, umwenda (ipamba), ikirahure, plastike (acrylic), n'ibiti (pinusi) bipima santimetero imwe na santimetero imwe.Kurandura ama coupons mbere yo kuyakoresha.Ingero zose zahagaritswe na autoclaving mbere yo kwandura bagiteri.
Muri ubu bushakashatsi, ingirabuzimafatizo za bagiteri zakwirakwijwe hejuru y’ubutaka ndetse no ku byapa bya agar.Ibibaho noneho bigahagarikwa muguhishurira ozone mugihe runaka kandi mugihe runaka mubyumba bifunze.Ku mutini.1 nifoto yibikoresho bya ozone.Imashini ya DBD plasma yahimbwe muguhuza electrode isobekeranye kandi yerekana ibyuma bitagira umwanda imbere n'inyuma ya mm 1 z'uburebure bwa alumina (dielectric).Kuri electrode isobekeranye, agace ka aperture nu mwobo byari mm 3 na mm 0.33.Buri electrode ifite ishusho izengurutswe na diameter ya mm 43.Umuvuduko mwinshi w'amashanyarazi menshi (GBS Elektronik GmbH Minipuls 2.2) wakoreshejwe mugukoresha ingufu za sinusoidal zingana na 8 kV hejuru ya pex kuri pex kuri 12.5 kHz kuri electrode isobekeranye kugirango itange plasma kumpera ya electrode.electrode isobekeranye.Kubera ko ikoranabuhanga ari uburyo bwo guhagarika gazi, sterisizione ikorerwa mu cyumba kigabanijwemo ingano mu bice byo hejuru no hepfo, birimo ingero zanduye na bagiteri hamwe na generator ya plasma.Igice cyo hejuru gifite ibyambu bibiri byo gukuramo no gukuramo ozone isigaye.Mbere yo gukoresha mubigeragezo, impinduka mugihe cya ozone yibanze mucyumba nyuma yo gufungura plasma yapimwe hakurikijwe uburyo bwo kwinjiza umurongo wa ecran ya 253.65 nm y'itara rya mercure.
.Igishushanyo cyakozwe hifashishijwe verisiyo ya OriginPro 9.0 (Porogaramu ya OriginPro, Northampton, MA, Amerika; https://www.originlab.com).
Ubwa mbere, muguhindura ingirabuzimafatizo zashyizwe kuri plaque ya agar hamwe na ozone, mugihe hahinduwe igihe cya ozone hamwe nigihe cyo kuvura, igihe gikwiye cya ozone hamwe nigihe cyo kuvura cyo kwanduza MDRO na C. diffile.Mugihe cyo kuboneza urubyaro, urugereko rwabanje gusukurwa numwuka wibidukikije hanyuma rwuzuzwa ozone mugukingura plasma.Ingero zimaze kuvurwa na ozone mugihe cyagenwe, pompe ya diafragm ikoreshwa mugukuraho ozone isigaye.Ibipimo byakoresheje icyitegererezo cy'umuco wuzuye w'amasaha 24 (~ 108 CFU / ml).Ingero zo guhagarika ingirabuzimafatizo (20 μl) zabanje gukurikiranwa inshuro icumi hamwe na saline sterile, hanyuma izo ngero zikwirakwizwa ku masahani agar yatewe na ozone mu cyumba.Nyuma yibyo, ingero zasubiwemo, zigizwe nintangarugero zerekanwe kandi zidahuye na ozone, zashyizwe kuri 37 ° C mumasaha 24 hanyuma zibarwa na koloni kugirango isuzume akamaro ko kuvuka.
Byongeye kandi, ukurikije uburyo bwo kuboneza urubyaro bwasobanuwe mu bushakashatsi bwavuzwe haruguru, hasuzumwe ingaruka zo kwanduza iryo koranabuhanga kuri MDRO na C. difficile zasuzumwe hifashishijwe udupapuro tw’ibikoresho bitandukanye (ibyuma bitagira umwanda, imyenda, ikirahure, plastike n’ibiti) bikoreshwa mu bigo by’ubuvuzi.Imico yuzuye yamasaha 24 (~ 108 cfu / ml) yakoreshejwe.Ingero zo guhagarika ingirabuzimafatizo (20 μl) zashizwemo inshuro icumi hamwe na saline sterile, hanyuma za coupons zinjizwa muri utwo dusimba twavanze kugirango dusuzume umwanda.Ingero zavanyweho nyuma yo kwibizwa mu muhogo wa dilution zashyizwe mu byokurya bya Petri sterile hanyuma zumishwa n'ubushyuhe bw'icyumba amasaha 24.Shira umupfundikizo wa petri kurugero hanyuma ubishyire mubyumba byipimisha.Kuramo umupfundikizo mubiryo bya Petri hanyuma werekane icyitegererezo kuri 500 ppm ozone muminota 15.Ingero zagenzuwe zashyizwe mu nama y’umutekano y’ibinyabuzima kandi ntizigeze zihura na ozone.Ako kanya nyuma yo guhura na ozone, ingero hamwe nicyitegererezo kidafite imirasire (ni ukuvuga kugenzura) byavanze na saline sterile ikoresheje imvange ya vortex kugirango itandukanya bagiteri hejuru.Ihagarikwa ryavuyeho ryakuweho inshuro 10 hamwe na saline sterile, nyuma y’umubare wa bagiteri zivanze zamenyekanye ku masahani y’amaraso (kuri bacteri zo mu kirere) cyangwa amasahani y’amaraso ya anaerobic ya Brucella (kuri Clostridium difficile) hanyuma ashyirwa kuri 37 ° C mu masaha 24.cyangwa mugihe cya anaerobic mumasaha 48 kuri 37 ° C muburyo bubiri kugirango umenye intangiriro ya inoculum.Itandukaniro ryimibare ya bagiteri hagati yubugenzuzi butamenyekanye hamwe nicyitegererezo cyagaragaye cyabazwe kugirango igabanye igabanywa ryimibare ya bagiteri (ni ukuvuga uburyo bwo kuboneza urubyaro) mugihe cyibizamini.
Ingirabuzimafatizo zigomba guhagarikwa ku isahani yerekana amashusho ya AFM;kubwibyo, disiki iringaniye kandi imwe ya mica disiki hamwe nubunini bugereranije buto kuruta ubunini bwakagari bukoreshwa nka substrate.Diameter n'ubunini bwa disiki byari mm 20 na mm 0,21.Kugirango ushikame neza ingirabuzimafatizo hejuru, hejuru ya mika yashizwemo na poly-L-lysine (200 µl), bigatuma ishiramo neza kandi membrane ikagabanuka nabi.Nyuma yo gutwikira poly-L-lysine, disiki ya mika yogejwe inshuro 3 hamwe na ml 1 ya deionised (DI) n'umwuka wumye ijoro ryose.Hanyuma, selile za bagiteri zashyizwe kumurongo wa mika zometse kuri poly-L-lysine ukoresheje umuti wa bagiteri utuje, usigara muminota 30, hanyuma hejuru ya mika wogejwe na ml 1 y'amazi ya deioni.
Kimwe cya kabiri cyicyitegererezo cyavuwe hamwe na ozone kandi morfologiya yubuso bwa plaque ya mika yuzuye VRE, CRAB na C. diffile spores yagaragaye hakoreshejwe AFM (XE-7, sisitemu ya parike).Uburyo bwa AFM bwo gukora bwashyizweho muburyo bwo gukanda, nuburyo busanzwe bwo gufata amashusho yibinyabuzima.Mu bushakashatsi, hakoreshejwe microcantilever yagenewe uburyo bwo kudahuza (OMCL-AC160TS, OLYMPUS Microscopy).Amashusho ya AFM yafashwe ashingiye ku gipimo cya scan ya 0.5 Hz bivamo ishusho ya 2048 × 2048 pigiseli.
Kugirango tumenye imiterere ya rezo ya DBD ifite akamaro kanini muguhindura ingumba, twakoze urukurikirane rwubushakashatsi dukoresheje MDRO (VRE, CRE, CRPA, na CRAB) na C. diffile kuburyo butandukanye bwa ozone hamwe nigihe cyo kwerekana.Ku mutini.1b yerekana igihe cya ozone yibanda kumurongo kuri buri kizamini nyuma yo gufungura igikoresho cya plasma.Imyitozo yiyongereye kuri logarithmique, igera kuri 300 na 500 ppm nyuma yiminota 1.5 na 2.5.Ibizamini byibanze hamwe na VRE byagaragaje ko byibuze bisabwa kugirango yanduze neza bagiteri ni 300 ppm ozone muminota 10.Rero, mubushakashatsi bukurikira, MDRO na C. diffile bahuye na ozone kubintu bibiri bitandukanye (300 na 500 ppm) no mubihe bibiri bitandukanye (iminota 10 na 15).Gukoresha uburyo bwa sterilisation kuri buri dose ya ozone nigihe cyo kwerekana igihe cyabazwe kandi cyerekanwe mu mbonerahamwe ya 1. Guhura na 300 cyangwa 500 ppm ozone mu minota 10-15 byatumye igabanuka rusange muri VRE ya 2 cyangwa irenga log10.Uru rwego rwo hejuru rwica bagiteri hamwe na CRE rwagezweho niminota 15 yo guhura na 300 cyangwa 500 ppm ozone. Kugabanuka kwinshi muri CRPA (> 7 log10) byagezweho hifashishijwe 500 ppm ya ozone kuminota 15. Kugabanuka kwinshi muri CRPA (> 7 log10) byagezweho hifashishijwe 500 ppm ya ozone kuminota 15. Высокое снижение CRPA (> 7 log10) было достигнуто при воздействии 500 частей на на гона в течение 15 мин. Kugabanuka kwinshi muri CRPA (> 7 log10) byagezweho hamwe no guhura na 500 ppm ozone muminota 15.暴露 于 500 ppm 的 臭氧 15 分钟 后 ， 可 大幅 降低 CRPA (> 7 log10)。暴露 于 500 ppm 的 臭氧 15 分钟 后 ， 可 大幅 降低 CRPA (> 7 log10)。 Существенное снижение CRPA (> 7 log10) после 15-минного воздействия କାନона снараций 500 ppm. Kugabanuka gukomeye muri CRPA (> 7 log10) nyuma yiminota 15 ihura na 500 ppm ozone.Kwica bidasubirwaho bacteri za CRAB kuri 300 ppm ozone; icyakora, kuri 500 ppm ozone, habayeho kugabanya 1.5 log10. icyakora, kuri 500 ppm ozone, habayeho kugabanya 1.5 log10. однако при прирации କାନона 500 частей на наблюдалось снижение> 1.5 log10. icyakora, kuri ozone yibanze ya 500 ppm, byagaragaye ko igabanuka rya> 1.5 log10.然而 ， 在 500 ppm 臭氧 下 ， 减少 了> 1.5 log10。然而 ， 在 500 ppm 臭氧 下 ， 减少 了> 1.5 log10。 Однако при прирации କାନона 500 частей на наблюдалось снижение> 1.5 log10. Nyamara, kuri ozone yibanze kuri 500 ppm, byagaragaye ko igabanuka rya> 1.5 log10. Kugaragaza C. diffile spores kuri 300 cyangwa 500 ppm ozone byatumye kugabanuka kwa 2.5 2.5. Kugaragaza C. diffile spores kuri 300 cyangwa 500 ppm ozone byatumye kugabanuka kwa 2.5 2.5. Воздействие на сп сп. Kumenyekanisha C. spicile spores kugeza 300 cyangwa 500 ppm ozone yatumye kugabanuka> 2.5 log10.将 艰难 梭菌 孢子 暴露 于 300 或 500 ppm 的 臭氧 中 导致> 2.5 log10 减少。 300 或 500 ppm 的 臭氧 中 导致> 2.5 log10 减少。 Воздействие на сп сп. Kumenyekanisha C. spicile spores kugeza 300 cyangwa 500 ppm ozone yatumye kugabanuka> 2.5 log10.
Hashingiwe ku bushakashatsi bwavuzwe haruguru, habonetse icyifuzo gihagije cyo kudakora bagiteri ku gipimo cya 500 ppm ozone mu minota 15.VRE, CRAB na C. spifile spores zapimwe kugirango zandure mikorobe ya ozone kubikoresho bitandukanye birimo ibyuma bitagira umwanda, imyenda, ikirahure, plastike nimbaho ​​zikoreshwa mubitaro.Uburyo bwiza bwo kuboneza urubyaro bugaragara mu mbonerahamwe ya 2. Ibinyabuzima byapimwe byasuzumwe kabiri.Muri VRE na CRAB, ozone ntiyagize ingaruka nziza hejuru yikirahure na plastike, nubwo igabanuka rya log10 ryikigereranyo cya 2 cyangwa byinshi byagaragaye ku byuma bitagira umwanda, imyenda nigiti.C. sporile spicile wasangaga irwanya imiti ya ozone kurusha ibindi binyabuzima byose byapimwe.Kugira ngo wige imibare ingaruka za ozone ku ngaruka zo kwica ibikoresho bitandukanye birwanya VRE, CRAB, na C. diffile, t-test yakoreshejwe mu kugereranya itandukaniro riri hagati y’umubare wa CFU kuri mililitiro mu kugenzura no mu matsinda y’ubushakashatsi ku bikoresho bitandukanye (Ishusho 2).imirongo yerekanaga itandukaniro rinini cyane, ariko itandukaniro ryibanze ryagaragaye kuri spore ya VRE na CRAB kuruta kuri C. diffile spore.
Scatterplot yingaruka za ozone mukwica bagiteri kwica ibikoresho bitandukanye (a) VRE, (b) CRAB, na (c) C. diffile.
Amashusho ya AFM yakorewe kuri ozone ivurwa kandi itavuwe VRE, CRAB, na C. diffile spores kugirango bige birambuye inzira ya gaz ya ozone.Ku mutini.3a, c na e byerekana AFM amashusho ya VRE itavuwe, CRAB na C. spicile spicile.Nkuko bigaragara mumashusho ya 3D, selile ziroroshye kandi ntizihinduka.Igishushanyo cya 3b, d na f cyerekana VRE, CRAB na C. sporile spicile nyuma yo kuvura ozone.Ntabwo byagabanutse gusa mubunini muri selile zose zapimwe, ariko ubuso bwazo bwarushijeho gukomera nyuma yo guhura na ozone.
AFM amashusho ya VRE itavuwe, MRAB na C. spicile spores (a, c, e) na (b, d, f) yavuwe na 500 ppm ozone kuminota 15.Amashusho yashushanijwe akoresheje Parike ya Sisitemu XEI verisiyo 5.1.6 (Porogaramu ya XEI, Suwon, Koreya; https://www.parksystems.com/102-ibicuruzwa/park-xe-bio).
Ubushakashatsi bwacu bugaragaza ko ozone ikorwa n’ibikoresho bya plasma ya DBD yerekana ubushobozi bwo kwanduza neza sporo ya MDRO na C. difficile, izwiho kuba intandaro y’indwara ziterwa n’ubuzima.Byongeye kandi, mu bushakashatsi bwacu, urebye ko kwanduza ibidukikije hamwe na MDRO na C. diffile spores bishobora kuba intandaro y’indwara ziterwa n’ubuzima, ingaruka za mikorobe ya ozone wasangaga zigenda neza ku bikoresho bikoreshwa cyane cyane mu bitaro.Ibizamini byo kwanduza byakozwe hakoreshejwe ibikoresho bya plasma ya DBD nyuma yo kwanduza ibikoresho nkibyuma bitagira umwanda, igitambaro, ikirahure, plastike ninkwi hamwe na MDRO na C. spicile spicile.Nkigisubizo, nubwo ingaruka zo kwanduza zitandukanye bitewe nibikoresho, ubushobozi bwo kwanduza ozone buratangaje.
Ibintu bikora cyane mubyumba byibitaro bisaba kwanduza, kurwego rwo hasi.Uburyo busanzwe bwo kwanduza ibintu nkibi ni ugusukura intoki hamwe nudukoko twangiza nka ammonium ya quaternary 13. Nubwo hubahirizwa byimazeyo ibyifuzo byo gukoresha imiti yica udukoko, MPO biragoye kuyikuraho hakoreshejwe isuku y’ibidukikije (ubusanzwe isukura intoki) 14.Kubwibyo, tekinoroji nshya irakenewe, nkuburyo budahuza.Kubera iyo mpamvu, habaye inyungu zo kwanduza gaze, harimo hydrogen peroxide na ozone10.Ibyiza bya disinfectant ya gaze ni uko bashobora kugera ahantu hamwe nibintu gakondo intoki zidashobora kugera.Hydrogene peroxide iherutse gukoreshwa mubuvuzi, icyakora hydrogen peroxide ubwayo ni uburozi kandi igomba gukemurwa hakurikijwe uburyo bukoreshwa neza.Plasma sterilisation hamwe na hydrogène peroxide isaba igihe kinini cyo kweza mbere yukwezi gutaha.Ibinyuranye, ozone ikora nka antibacterial agent yagutse, ikora neza kurwanya bagiteri na virusi zirwanya izindi zanduza 8,11,15.Byongeye kandi, ozone irashobora kubyazwa umusaruro uva mu kirere cyo mu kirere kandi ntisaba imiti y’ubumara ishobora kwangiza ikirere cyangiza ibidukikije, kubera ko amaherezo icika ogisijeni.Ariko, impamvu ituma ozone idakoreshwa cyane nka disinfectant niyi ikurikira.Ozone ni uburozi kubuzima bwabantu, kubwibyo kwibanda kwayo ntibirenza 0.07 ppm ugereranije mugihe cyamasaha arenga 816, bityo steriseri ya ozone yarakozwe kandi ishyirwa kumasoko, cyane cyane mugusukura imyuka isohoka.Birashoboka kandi guhumeka gaze no kubyara impumuro idashimishije nyuma yo kwanduza 5.8.Ozone ntabwo yakoreshejwe cyane mubigo byubuvuzi.Nyamara, ozone irashobora gukoreshwa neza mubyumba byo kuboneza urubyaro hamwe nuburyo bukwiye bwo guhumeka, kandi kuyikuraho birashobora kwihuta cyane ukoresheje catalitike ihindura.Muri ubu bushakashatsi, twerekana ko sterilizeri ya plasma ozone ishobora gukoreshwa mu kwanduza indwara.Twateje imbere igikoresho gifite ubushobozi bwo kuboneza urubyaro, gukora byoroshye na serivisi yihuse kubarwayi bari mubitaro.Mubyongeyeho, twateje imbere uburyo bworoshye bwo kuboneza urubyaro bukoresha umwuka w’ibidukikije nta kiguzi cyinyongera.Kugeza ubu, hari amakuru adahagije kubisabwa byibura ozone kugirango MDRO idakora.Ibikoresho bikoreshwa mubushakashatsi bwacu biroroshye gushiraho kandi bifite igihe gito cyo gukora kandi biteganijwe ko bizagira akamaro mugukoresha ibikoresho kenshi.
Uburyo bwibikorwa bya bagiteri yica ozone ntibisobanutse neza.Ubushakashatsi bwinshi bwerekanye ko ozone yangiza uturemangingo twa bagiteri, biganisha ku kuva mu nda ndetse na lysis ya selile amaherezo 17,18.Ozone irashobora kubangamira ibikorwa byimikorere ya selile ikora reaction ya matsinda ya thiol kandi irashobora guhindura purine na pyrimidine muri acide nucleic.Ubu bushakashatsi bwerekanaga morphologie ya VRE, CRAB, na C. spicile spore mbere na nyuma yo kuvura ozone isanga ko bitagabanutse mu bunini gusa, ahubwo byanabaye bibi cyane hejuru, byerekana kwangirika cyangwa kwangirika kwinyuma.nibikoresho by'imbere kubera gaze ya ozone ifite ubushobozi bukomeye bwa okiside.Ibi byangiritse birashobora gutuma selile idakora, bitewe nuburemere bwimpinduka za selile.
C. sporile spicile biragoye kuyikura mubyumba byibitaro.Spore iguma ahantu bamennye 10,20.Mubyongeyeho, muri ubu bushakashatsi, nubwo logarithmic ntarengwa yagabanutse inshuro 10 umubare wa bagiteri ku masahani ya agar kuri 500 ppm ozone mu minota 15 yari 2.73, ingaruka za bagiteri ziterwa na ozone ku bikoresho bitandukanye birimo spore C .difficile yagabanutse.Kubwibyo, ingamba zitandukanye zirashobora gutekerezwa kugabanya kwandura C. kwandura mubuzima.Kugira ngo ukoreshwe mu byumba C. bitandukanya ibyumba gusa, birashobora kandi kuba byiza guhindura igihe cyo guhura nuburemere bwo kuvura ozone.Byongeye kandi, tugomba kuzirikana ko uburyo bwa ozone bwo kwanduza budashobora gusimbuza rwose isuku yintoki zisanzwe hamwe nudukoko twangiza hamwe ningamba zirwanya mikorobe, kandi birashobora no kuba ingirakamaro mugucunga C. diffile 5.Muri ubu bushakashatsi, imikorere ya ozone nka disinfectant itandukanye kubwoko butandukanye bwa MPO.Imikorere irashobora guterwa nibintu byinshi nkicyiciro cyo gukura, urukuta rw'akagari, hamwe nuburyo bwo gusana21,22.Impamvu yingaruka zinyuranye za ozone hejuru ya buri kintu gishobora guterwa no gukora biofilm.Ubushakashatsi bwibanze bwerekanye ko E. faecium na E. faecium byongera ibidukikije iyo bihari nka biofilms23, 24, 25. Nyamara, ubu bushakashatsi bwerekana ko ozone igira ingaruka zikomeye za bagiteri kuri MDRO na C. spicile spicile.
Intambamyi yubushakashatsi bwacu nuko twasuzumye ingaruka zo kugumana ozone nyuma yo gukosorwa.Ibi birashobora gutuma umuntu arengana umubare wa selile zifatika.
Nubwo ubu bushakashatsi bwakozwe kugirango hamenyekane imikorere ya ozone nka disinfectant mu bitaro, biragoye guhuza ibisubizo byacu mubitaro byose.Niyo mpamvu, ubushakashatsi burakenewe kugira ngo hakorwe iperereza ku bijyanye no guhuza iyi DBD ozone sterilizer mu bitaro nyabyo.
Ozone yakozwe na reaction ya DBD plasma irashobora kuba ibintu byoroshye kandi bifite agaciro ko kwanduza MDRO na C. diffile.Gutyo, kuvura ozone birashobora gufatwa nkuburyo bwiza bwo kwanduza ibidukikije ibitaro.
Imibare ikoreshwa kandi / cyangwa yasesenguwe mubyigisho byubu iraboneka kubanditsi babisabwe babisabye.
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