Ati: “Ntuzigere ushidikanya ko itsinda rito ry'abaturage batekereza, bitanze bashobora guhindura isi.Nkako, ni yo yonyine ihari. ”
Inshingano za Cureus nuguhindura uburyo bumaze igihe cyo gutangaza ubuvuzi, aho gutanga ubushakashatsi bishobora kuba bihenze, bigoye, kandi bitwara igihe.
Plasma ikungahaye kuri plasma / prp, kuvugurura ingirabuzimafatizo, gukora platine, kuvura glucose, kuvura, kuvura,
Tanga iyi ngingo nka: Harrison TE, Bowler J, Reeves K, n'abandi.(Gicurasi 17, 2022) Ingaruka ya glucose kumubare wa platine nubunini: ingaruka zubuvuzi bushya.Umuti 14 (5): e25081.doi: 10.7759 / gukiza.25081
Plasma ikungahaye kuri plasma (PRP) hamwe na hypertonic glucose ibisubizo bikunze gukoreshwa mugutera inshinge mubuvuzi bushya, rimwe na rimwe hamwe.Ingaruka ya glucose ya hypertonic kuri lysis no gukora ntabwo byigeze bivugwa.Twagerageje ingaruka ziterwa na glucose hejuru ya platelet na erythrocyte, hamwe nubunini bwa selile muri PRP namaraso yose (WB).Kugabanuka byihuse kubice bya platine byabaye hamwe nuruvange rwa glucose ruvanze na PRP cyangwa amaraso yose, bihuye na lysis igice. Nyuma yiminota yambere, ibara rya platine ryagumye rihamye, byerekana icumbi ryihuse rya platine isigaye kurenza hypertonicity ikabije (> 2000 mOsm). Nyuma yiminota yambere, ibara rya platine ryagumye rihamye, byerekana icumbi ryihuse rya platine isigaye kurenza hypertonicity ikabije (> 2000 mOsm). После первой минуты количестро тромбоцитов увасос с стабильным, что указывает на быструю аккомодацию остаточных тромбоцитов до экстремемналного (> 2000 мОсм) гипертонуса. Nyuma yiminota yambere, umubare wa platel wagumye uhagaze neza, byerekana icumbi ryihuse rya platine isigaye kugeza hypertonicity ikabije (> 2000 mOsm).第一 分钟 后 ， 血小板 计数 保持 稳定 ， 残余 血小板 2000 2000 2000> 2000 mOsm） 高渗 状态。2000 mOsm） 高渗 状态。 После первой минуты количестро тромбоцитов увасос с стабильным, что указывает на быструю адаптацию остаточных тромбоцитов к экстремемнному (> 2000 мОсм) гиперосмоларному состоянию. Nyuma yumunota wambere, umubare wa platine wagumye uhagaze neza, byerekana ihinduka ryihuse ryibisigisigi bisigaye kuri hyperosmolar bikabije (> 2000 mOsm).Glucose yibanze kuri 25% no hejuru yayo byatumye ubwiyongere bugaragara mubunini bwa platine (MPV), byerekana icyiciro cya mbere cyo gukora platine.Iyindi nyigisho irakenewe kugirango hamenyekane niba lysis ya platisite cyangwa activation ibaho kandi niba inshinge ya hypertonic glucose yonyine cyangwa ifatanije na PRP ishobora gutanga inyungu zubuvuzi.
Mu myaka ya za 1950, umunyamerika wabaga George Hackett yavumbuye ko ashobora kugabanya burundu ububabare bw’umugongo n’umugongo ku barwayi benshi atera igisubizo cyinshi mu mitsi no mu mitsi.Ubushakashatsi yakoze ku nkwavu bwerekanye ko ubuvuzi yise ubuvuzi bukabije, bwatumye imitsi yaguka kandi ikomera.Ubushakashatsi bw’amateka bwemeje ko kolagen nshya ikorwa muri iki gikorwa [1].
Mu myaka mike yambere, ibisubizo byinshi bitandukanye byo kugabura byageragejwe.Mu myaka ya za 90, abimenyereza benshi babonaga ko glucose yibanze cyane ko aribwo buryo bwizewe kandi bwiza.Nyamara, uburyo bwibikorwa ntibusobanutse.
Ubushakashatsi buke mu mavuriro bwakozwe mu kinyejana cya 20 nyuma ya Hackett.Nyamara, mu myaka ya za 2000 hongeye gushimishwa kandi hasuzumwe ibizamini byinshi by’amavuriro byo kuvura indwara nyinshi byarangiye kugira ngo bivure ububabare bwo mu mugongo [2], osteoarthritis yo mu ivi [3], na epicondylitis yo ku ruhande [4].
Kuvugurura imyenda bisaba uruhare rwingirabuzimafatizo.Kubwibyo, kwibanda cyane kwa glucose bigomba gutera uburyo bwo kwimuka, kwigana, no gutandukanya ingirabuzimafatizo.Turakekeranya ko platine ishobora gukora nk'intumwa kandi ko glucose yuzuye ishobora gutuma platine irekura cytokine hamwe nimpamvu zikura, bityo bigatuma habaho uburyo bushya bwo kuvugurura ibintu, cyane cyane kwimuka kw ingirabuzimafatizo mu turere twinshi twa glucose.
Gukora platel buri gihe bibanziriza kwiyongera kwa calcium yo mu nda [5].Liu n'abandi.muri 2008 yerekanye ko urugero rwa glucose rwongera ibikorwa byinzira zigihe gito zishobora kwakirwa muburyo bwa Canonical ubwoko bwa 6 (TRPC6) mumyanya ya plasma membrane, bigatuma habaho kwinjiza ioni ya calcium muri platine [6].Ubundi bushakashatsi bwerekanye ko guhura na microtubule marginal zone ya calcium ion bitera kuruhuka, kwaguka, no guhindura imiterere ya marginal, ari nako bitera ihinduka ryimiterere kuva disiki ikajya kuri serefegitire, bikavamo ubunini bwa platine (MPV) [7].
Igitekerezo cyacu muri ubu bushakashatsi ni uko guhura na platine ku bwinshi bwa glucose bigira ingaruka kuri microtubule marginal zone ndetse n’ibidukikije bidasanzwe, bigatuma MPV yiyongera.
Abitabiriye amahugurwa bose bashyize umukono ku mpapuro zabemereye nyuma yo gusobanura ibisobanuro birambuye mbere yo kwakira izo ngero.Muri ubu bushakashatsi, hifashishijwe urugero rwa PRP gusa hamwe na hematocrit irenga 2% kugira ngo erythrocyte (erythrocyte) ibare kandi bivuze ingano ya corpuscular ya selile yamaraso itukura (MCV) ishobora gushyirwamo kugereranya.
Ubushakashatsi bwakozwe mu byiciro bine, icyiciro cya mbere ni PRP naho ibyiciro bisigaye byari amaraso yose (Imbonerahamwe 1).Nkuko byasobanuwe mbere [8], imbaraga zose zijyanye na centrifugal (RCF, g-force) zabazwe uhereye hagati (Rmid, muri cm) winkingi yamaraso muri siringi ya centrifugal.Twahisemo gukoresha MPV nk'ikimenyetso cyo gukangurira platel no kubara platelet nk'ikimenyetso cyerekana lisiti ishobora kuba, byombi bishobora gupimwa byoroshye kubasesengura bisanzwe.
Mu cyiciro cya mbere, abakorerabushake 47 batanze urugero rw'amaraso - umuyoboro umwe wa aside yitwa Ethylenediaminetetraacetic (EDTA) hamwe na PRP imwe y'amaraso yose (anticoagulated with sodium citrate (NaCl, 3%)) (Imbonerahamwe 1).Shira rocker muri tube ako kanya.Kubara amaraso yuzuye (CBC) byakorewe ku cyitegererezo cya EDTA muri bitatu, naho NaCl yasesenguwe muri bitatu kugirango isesengura CBC, hanyuma PRP itegurwa nuburyo butandukanye bwasobanuwe haruguru [8].Ingero zose za PRP zateguwe na centrifugation kuri 900-1000 g.Kuvanga buri cyitegererezo cya PRP kuri mixeur ya vortex kumasegonda 5-10, hanyuma ugabanye alikoti eshanu 0,5 muri tubes.
Kugirango hamenyekane ingaruka ziterwa na platine hejuru yubunini bwa glucose, bingana (0,5 ml) ya 0%, 5%, 12.5%, 25%, na 50% glucose mumazi byavanze nicyitegererezo cya platine kugirango babone 0%, 2.5% 6.25%, 12.5% ​​na 25% byibanze bya glucose bivanze hanyuma bavange imiyoboro kumashanyarazi yikizamini muminota 15.TAC ya buri mvange yasesenguwe muri bitatu nyuma yiminota 15.Umubare wa platelet (PLT), kubara RBC, MCV, na MPV wagereranijwe kuri buri muyoboro, kandi bivuze ko kubara platine, kubara RBC, MCV, na MPV byabazwe kuri PRP zose.
Nyuma yicyiciro cya mbere cyo gukusanya amakuru kirangiye, twabonye ubwiyongere bugaragara mububiko bwa platel muri PRP nyuma yo kongerwaho D50W.PRP platine ntabwo isobanura byanze bikunze platine zose mumaraso, kandi PRP itandukanye na WB.Kubwibyo, twahisemo gukora ikizamini cya kabiri cyingaruka zo kongera D50W mumaraso yose.
Ku cyiciro cya kabiri, twahisemo urugero rwa 30 rushingiye kubisubizo bivuye murukurikirane rwa mbere, nkuko byasobanuwe mu gice cyisesengura.Muri uru ruhererekane, abakorerabushake 20 batanze urugero rw'amaraso (Imbonerahamwe 1).Amaraso yose (1.8 ml) yakuwe muri siringi ya ml 3 hanyuma anticoagulée hamwe na 0.2 ml 40% NaCl.Siringe yamaraso yose yavanze mumasegonda atanu hamwe na mixeur ya vortex hanyuma CBC isesengurwa muri bitatu.Nyuma yisesengura, maraso anticoagulée yongerewe kuri ml 2 ya glucose ya 50% muri seringe ya ml 5 (kwibanda kwa glucose ya nyuma yari hafi 25% (D25) hanyuma ishyirwa mu muyoboro wa shake mu minota 30. Nyuma yiminota 30, D25 / CBC muri siringi ya WB yasesenguwe inshuro eshatu. Kubara platine, kubara RBC, MCV, na MPV kuri siringi byagereranijwe, kandi bivuze ko MCV yabibwiwe, kandi bivuze ko PLV,
Kubera ko platine mu maraso yose ikunze guhura na glucose ya hypertonic mugihe cyo kuvura glucose ikabije bitewe no gutera inshinge nkeya, kandi ntibisanzwe guhuza PRP na glucose hypertonic mbere yo gutera inshinge, twahisemo kwiga glucose hypertonic dufatanije na WB mugice cya 1. Intambwe ya gatatu n'iya kane.Kuri buri cyiciro, abakorerabushake 20 batanze ml 7-8 ya ACD-A (aside irimo citrate ya trisodium (22.0 g / l), aside citricike (8.0 g / l) na glucose (24.5 g / l), umuti wa dextrose citrate) mu kurwanya anticagulants (Imbonerahamwe 1).Gusa imvange ya glucose irenga 12.5% ​​yakoreshejwe kugirango hamenyekane ijanisha ntarengwa rijyanye no kwiyongera kwa MPV.Ku cyiciro cya gatatu, ml 1 yamaraso ishyirwa mumiyoboro yipimisha.Noneho vanga amaraso kumuvanga wa vortex mumasegonda 10 wongeyeho ml 1 ya 30% glucose, 40% glucose, cyangwa 50% glucose kumuyoboro kugirango ubone glucose yanyuma ya 15%, 20%, na 25%.Amaraso ya glucose yasesenguwe kuri CBC ako kanya nyuma yo kuvanga no gusubiramo buri minota ibiri muminota 30.
Mugihe cyo kuvanga kwambere, kongeramo 1: 1 hypertonic glucose na WB cyangwa PRP byerekana platine yibitekerezo biri hejuru ya 25% mumasegonda menshi.Mu ntambwe ya kane, kugirango dusuzume ingaruka za glucose ya hypertonique hamwe nubushakashatsi bwambere bwambere bwo hejuru no gusuzuma urugero rwo hejuru rwingaruka za glucose, twongeyeho amaraso make kuri D25W cyangwa D50W.Shira ml 1 ya D25W cyangwa D50W mumiyoboro hanyuma wongeremo 0.2 ml ya WB mugihe uzunguruka icyitegererezo kumasegonda 10.Muri ibi bihe, amaraso yahuye na glucose yibitekerezo hafi 20% hejuru yubushakashatsi bwa nyuma, aho kuba 50% hejuru yubushakashatsi bwa nyuma nko mu cyiciro cya 3, bigatuma glucose ya nyuma ya 20.8% na 41,6%.Ingero zivanze zasesenguwe mugihe kimwe nkintambwe ya 3.
Mu ntambwe yambere ya buri cyiciro cya glucose ya glucose, hafashwe ingero 30 kuko aribwo bunini bwakorewe ubushakashatsi bwikigereranyo [9].Iyo buri cyiciro kirangiye (harimo icyiciro cya mbere), suzuma bihagije ingano yicyitegererezo ukoresheje formula yakoreshejwe kugirango umenye ingano yicyitegererezo ikenewe kugirango ugereranye uburyo impinduka zikomeza guhinduka mubaturage umwe.Inzira n = Z2 x SD2 / E2.Muri uku kugereranya, Z ni Z-amanota, SD ni gutandukana bisanzwe, na E ni ikosa ryifuzwa [10].Alpha yacu ni 0.05, ihuye na Z agaciro ka 1.96, kandi turateganya ikosa rya 5 (ku ijana).Kubwibyo dukemura kuri n = (1.962 x SD2) / 52.Ibisubizo byerekanaga ko ingano yicyitegererezo isabwa kuri buri cyiciro yari nto ugereranije numubare nyawo wakusanyijwe.
Mugihe cya 1, 3 na 4 ukoresheje glucose irenze imwe, ingaruka ziterwa na glucose zitandukanye zasesenguwe mugereranya ihinduka ryibice hagati yigihe 0 na buri gihe cyakurikiyeho (icyiciro cya 1 kuminota 15, igihe cya 3 kuminota 15).na bine kumasegonda 15, hanyuma buri minota ibiri.) Igipimo cyo guhindura buri gihe cyagereranijwe ukoresheje Mann-Whitney U-test kuko amakuru ntiyakurikiranye isaranganya risanzwe nkuko byagenwe nikizamini gisanzwe cya Shapiro-Wilk.Kubera ko isesengura rya 1 kugeza kuri 1 ryamatsinda menshi (atanu) ryakozwe mu ntambwe ya mbere, iya gatatu n'iya kane (eshanu zose hamwe), hakosowe Bonferroni kugira ngo ihindure agaciro ka alfa yifuzwa ≤0.01 ariko ntabwo ≤0.05.
Kugabanya umubare wa platel hamwe na hypertonic dextrose yose hamwe no kwiyongera kwa MPV muri platine ya PRP kuri> 12.5% ​​ya dextrose yibanze: Umubare wa platine wa PRP wazamutse uva kumurongo umwe kugeza kuri gatanu ugereranije namaraso yibanze yose, bitandukanye nuburyo (buterekanwa). Kugabanya umubare wa platel hamwe na hypertonic dextrose yose hamwe no kwiyongera kwa MPV muri platine ya PRP kuri> 12.5% ​​ya dextrose yibanze: Umubare wa platine wa PRP wazamutse uva kumurongo umwe kugeza kuri gatanu ugereranije namaraso yibanze yose, bitandukanye nuburyo (buterekanwa). Уменьшение количества тромбоцитов при всех киранх гипертонической декстрозы и увеличение MPV в тромб ахтах PRP при зависимости от метода (не показано). Kugabanuka kwa platel kuri hypertonic dextrose yose hamwe no kwiyongera kwa MPV muri platine ya PRP kuri> 12.5% ​​ya dextrose yibanze: Umubare wa PRP wongeyeho inshuro 1-5 ugereranije namaraso yibanze yose, bitewe nuburyo (buterekanwa). ).在> 12.5% ​​的 葡萄糖 浓度 下 ， 所有 浓度 高渗 ， PRP 血小板 中 MPV 增加 ： 与 P P P P P P P P 倍 的 倍 （（ Kuri> 12.5% ​​bya glucose yibanze, glucose nyinshi igabanya umubare wamaraso, PRP yamaraso MPV yiyongera: ugereranije na 与 基线 全 血, umubare wamaraso PRP wiyongera kuva kuri 1 kugeza kuri 5 ugereranije nubushakashatsi (ntibisobanuwe). При занх глюкозы> 12,5% вс киьнной крови, вависимости от метода (не описано). Kwibanda kwa glucose> 12.5%, glucose yibipimo bya glucose byagabanije umubare wa platel kandi byongera MPV muri platine ya PRP: Umubare wa PRP wongeyeho inshuro 1 kugeza kuri 5 ugereranije n’ibanze byuzuye byamaraso, bitewe nuburyo (nkuko byasobanuwe).Igishushanyo 1 kirerekana ko umubare wa platine wagabanutseho hafi 75% nyuma yo kuyungurura mumazi na 20-30% nyuma yiminota 15 yo kuyungurura hamwe na glucose itandukanye ugereranije na PRP yibanze hamwe na 1: 1 byahinduwe kubunini (1- k1 hamwe no gukosora amajwi).k -1 ubworozi) .1 korora).
Umubare w'utugingo ngengabuzima muri buri dilution ugaragazwa nkigice cyumubare wambere mbere yo kuyungurura.
MPV yagabanutse cyane mu gihe cyo kubyara PRP, nta yandi mahinduka yagabanijwe kugeza kuri 12.5% ​​mu mazi cyangwa glucose (harimo 25% ya PRP glucose ivanze) kandi yiyongereyeho hejuru ya 20% nyuma yo kuyungurura 50% ya glucose (Ishusho .2).).Ibinyuranye, erythrocytes yerekanaga ko nta mpinduka nini yagaragaye mubunini iyo ari yo yose itari H2O.
Impuzandengo ya selile muri buri dilution igaragazwa nkijanisha ryijwi ryambere mbere yo kuyungurura.
Kugabanuka nkukwo ariko kutagaragara kugabanuka kwa platel no kwiyongera kwa CVR byagaragaye muri BC byagaragaye glucose 50% (gukora glucose 25%).Imbonerahamwe ya 2 igereranya imibare ya selile nubunini bwingirabuzimafatizo mumaraso yose yavanze muri 50% dextrose hamwe nicyiciro cya 1 PRP yatanzwe muri 50% dextrose.Impinduka mu mibare ya RBC na RBC MCV ntabwo zagaragaye kandi ntabwo aribyo twibanzeho.
SD = gutandukana bisanzwe, MD = bisobanura itandukaniro hagati yitsinda, SE = gutandukana bisanzwe gutandukanya itandukaniro, RBC = erythrocytes, PLT = platine, PRP = plasma ikungahaye kuri plasma, WB = amaraso yose
Nyuma yo kongeramo D50W muri WB, ijanisha ryagabanijwe ryahinduwe rya platine yari 7.7% (310 ± 73 na 286 ± 96) ugereranije na 17.8% yo gukwirakwiza PRP muri D50W (664 ± 348 na 544 ± 277).MPV WB yiyongereyeho 16.8% (kuva 10.1 ± 0.5 igera kuri 11.8 ± 0,6), naho MPV PRP yiyongereyeho 26% (9.2 ± 0.8 na 11.6 ± 0. 7). Nubwo itandukaniro riri hagati yo kugabanya umubare wa platel no kwiyongera kwa MPV byari byinshi cyane hamwe na PRP, impinduka zo kugabanya umubare wa platine muri WB zari hafi cyane (310 ± 73 kugeza 286 ± 96 (-7.7%); p = .06) kandi kwiyongera kwa MPV byari bikomeye (10.1 ± 0.5 kugeza 11.8 ± 0.6 (+16.8) p <.001). Nubwo itandukaniro riri hagati yo kugabanya umubare wa platel no kwiyongera kwa MPV byari byinshi cyane hamwe na PRP, impinduka zo kugabanya umubare wa platine muri WB zari hafi cyane (310 ± 73 kugeza 286 ± 96 (-7.7%); p = .06) kandi kwiyongera kwa MPV byari bikomeye (10.1 ± 0.5 kugeza 11.8 ± 0.6 (+16.8) p <.001).Nubwo itandukaniro riri hagati yo kugabanya kubara kwa platine no kwiyongera kwa CVR byari byinshi cyane hamwe na PRP, impinduka zo kugabanuka kwa platine muri WB zari zikomeye (310 ± 73 kugeza 286 ± 96 (-7.7%); p = 0.06).увеличение MPV было значительным (от 10,1 ± 0,5 до 11,8 ± 0,6 (+16,8) p <0,001). kwiyongera kwa MPV byari bifite akamaro (kuva 10.1 ± 0.5 kugeza 11.8 ± 0,6 (+16.8) p <0.001).P PRP 在 血小板 计数 减少 和 MPV 增加 方面 的 平均 差异 B B B B B B B 0 0 0 （（310 ± 73 至 286 ± 96 (-7.7%) ； p = .06） 和 V <.001）。P PRP 在 血小板 计数 和 和 增加 方面 的 差异 显 着 （计数 0 0 0 310 ± 73 至 286 ± 96 (-7.7%) ； p = .06） 和 MPV 的 是<.001）。Guhindura kugabanuka kwa platelet muri WB byari hafi cyane (kuva 310 ± 73 kugeza 286 ± 96 (-7.7%); p = 0.06), nubwo PRP yari ifite itandukaniro rinini risobanura itandukaniro riri hagati yo kugabanuka kwa platine no kwiyongera kwa MPV.no kwiyongera kwa MPV byari bifite akamaro.(от 10,1 ± 0,5 до 11,8 ± 0,6 (+16,8) р <0,001). (kuva 10.1 ± 0.5 kugeza 11.8 ± 0,6 (+16.8) p <0.001).
Ubwinshi bwa glucose ya 20% byasabwaga kugirango habeho impinduka zikomeye muri MPV, ariko ihinduka rya MPV ryagaragaye cyane kuri concentration ya nyuma ya 25%.Igihombo cya platel cyarahagaze nyuma yo kugabanuka kwambere.Twabonye ko kugabanuka kwa CVR kwambere, ariko, CVR yagaruwe byihuse kuri 25% ya glucose yanyuma, ibyo bikaba byari hejuru cyane kurwego rwa CVR rwagaragaye kuri glucose ya nyuma ya 20% na 15% (Ishusho 3 no ibumoso bwimbonerahamwe ya 3; agasanduku gicucu).erekana p-gaciro ≤ alpha hamwe na Bonferroni ikosora ya 0.01).Habayeho kandi kugabanuka kwambere kumubare wa PLT, byagaragaye mugice cyambere cya 0-15 s, hanyuma ukomeza guhagarara neza (kuva 15 s kugeza 30 min; ibumoso bwimbonerahamwe 4).
Kwiyongera kwinshi kwa glucose kumaraso yose byatumye igabanuka ryambere rya MPV hanyuma hakurikiraho gukira guterwa na 20%.Umugani werekana ubunini bwa glucose nyuma yo kuyungurura.D15, D20 na D25 byakozwe muburyo bwa 1: 1.D21 na D41 byakozwe kuri 1: 5.
Imbonerahamwe 4 irerekana ihinduka ryimibare ya platel iyo ivanze muri glucose ya hypertonic.Twabonye umubano ushingiye ku kugabanuka hagati yimibare ihita igabanuka kumibare ya PLT kuri 1: 1 no kuri 1: 5.Ugereranije ihindagurika rya 1: 1 nkitsinda rimwe hamwe na 1: 5, itsinda rya 1: 1 ryagabanutse ako kanya umubare wa platel munsi ya 1: 5 itsinda 66 ± 48.000 (23%) na 99 ± 69.000 (37%)., p = 0.014) mu itsinda rya 1: 5.Nyuma yo kugabanuka kwambere kumwanya wambere wapimwe, platelet ibara nkijanisha rya glucose ihagaze neza (Ishusho 4).
Iyo amaraso yose yongewe kuri glucose mubipimo bya 1: 1, umubare wa platel ugabanukaho 25%.Ariko, mugihe amaraso yose yongeyeho ku kigereranyo cya 1: 5, kugabanuka kwabaye kwinshi - hafi 50%.
41% glucose yiyongereye MPV byihuse kandi bitangaje kurenza 25% cyangwa 21%.Ibisubizo bya MPV byerekanwe ku gishushanyo cya 3. Ku zindi mpinduka zose, nta kugabanuka kwambere kwa MPV byagaragaye nyuma yo kongeramo glucose 50%.Iyo ukoresheje glucose 25% (kwibanda kuri glucose 20.8% mugihe cyanyuma), ihinduka rya MPV ryagereranijwe nimpinduka ya 20% glucose mugihe cya 1: 1 (Ishusho 3).Nubwo impinduka muri MPV zabanje kuba nyinshi kuri 41% zivanze kuruta kuri 25%, itandukaniro rya MPV hagati ya 41% na 25% nyuma yiminota 16 ntiryari rikigaragara (Imbonerahamwe 3, iburyo).Birashimishije kandi ko 25% glucose yiyongereye MPV neza kurenza 20.8%.
Ibi mubushakashatsi bwa vitro byemeje igice hypothesis. Yerekanaga ibibyimba bya platine igice cya dextrose ivanze, icumbi ryihuse rya platine kuri hypertonicity ikabije, hamwe no kuzamuka cyane kwa MPV hasubijwe> 25% bya hypertonic dextrose. Yerekanaga ibibyimba bya platine igice cya dextrose ivanze, icumbi ryihuse rya platine kuri hypertonicity ikabije, hamwe no kuzamuka cyane kwa MPV hasubijwe> 25% bya hypertonic dextrose. Он показал данный частичный лизис тромбоцитов примесу декстрозы, бстрстую аккомодацию тромбоцитов до экстремального гипертонуса и значительное повшенение MPV в ответ на гиоффическую Yerekanye ibishobora kuba byitwa lysis hamwe na dextrose, icumbi ryihuta rya platel kugeza hypertonicity ikabije, hamwe no kwiyongera gukabije kwa MPV hasubijwe urwego rwa hypertonic dextrose> 25%.25% 出 通过 V V 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 V V 25它 显示 出 通过 葡萄糖 潜在 的 部分 血小板 血小板 快速 25> 25% 浓度 高渗 葡萄糖 时 时 mpv 显 着 。。。。。 Он показывает କାନный частичный лизис тромбоцитов смесями с глюкозой, бструю адаптацию тромбоцитов к экстремальному гипертонусу и значительное увеличение MPV в ответ на Irerekana ibibyimba bimwe na bimwe bya lysis bivangwa na glucose ivanze, imihindagurikire yihuse ya platine na hypertonicity ikabije, hamwe no kwiyongera gukabije kwa MPV hasubijwe glucose ya hypertonic> 25%.Ubwiyongere bwambere bwari bwinshi kuri glucose ya 41,6%, ariko kwiyongera kwa MPV byegereye 25% glucose guhura nyuma yiminota 20 nyuma yo guhura.
Ubwinshi bwa platine bugira ingaruka kuri glucose.Twabonye ko umubare wa PLT wagabanutse kuri glucose zose.Kugabanuka gukabije mumibare ya platine muri H2O (0%) ihindagurika rya serivise ya PRP irashobora kuba ifitanye isano na osmotic lysis.Ubundi, ibi birashobora kuba igihangano cyatewe no gufata platine, ariko ibi bitandukanye no kubura impinduka za MPV kuriyi dilution.Ubu bushakashatsi bivuze ko platine zimwe zumva cyane hypoosmolarity.
Muri 1: 1 zose za glucose, umubare wa PLT wagabanutseho 20-30%, ndetse na D5W (hypotonic kuri 252 mOsm), ibyo bikaba bishobora kwerekana ingaruka zidasanzwe za glucose, kubera ko PLT na MPV byombi bitigeze bihinduka mugihe cyikubye inshuro eshatu kwiyongera.glucose.kuva D5W kugeza D25W.Mubyukuri, kwibanda kwa PLT byakunze kwiyongera gato hamwe no kwiyongera kwa osmolarite.
Kugabanuka kwa PLT hagati ya 1: 1 na 1: 5 bivuze ko ingaruka zo guseswa ziterwa na glucose yibanze kandi yanyuma.Niba byaterwaga gusa nubushakashatsi bwibanze, noneho umuntu yakwitega kubona itandukaniro ryo kugabanuka kwa PLT hagati ya 1: 1.Ariko ntitubikora.Niba ingaruka ya lysis iterwa gusa na glucose yanyuma, ntabwo rero dutegereje itandukaniro ryinshi hagati ya 20% 1: 1 na 20.8% 1: 5.Kandi nyamara twarabikoze.
Niba igihombo cya platel kibaye kubera lysis ya platine, hashyirwaho lysate igice, nyuma ya cytokine nibintu bikura bikarekurwa mubidukikije bidasanzwe.Ubushakashatsi bwinshi bwerekanye ko lysate ya platel ikora neza nka PRP nkigisubizo cyo gukwirakwiza [11].PRP ubwayo yerekanwe ko ari igisubizo cyiza cyo kuvura ikwirakwizwa [12-14].
Amashanyarazi adakora azenguruka muburyo bwa disiki ishimangirwa nuburyo bwinshi bwimbere.Mugihe cyo gukora, bafata imiterere myinshi cyangwa amibe, bigatuma ubwiyongere bwijwi.Ubwiyongere bw'ijwi busaba kwiyongera k'ubuso, ibyo bikaba ibisubizo byo gukuramo sisitemu ifunguye (OCS) no kongeramo granules ya exocytic muri membrane.Hasigaye kumenya niba ubwiyongere bwa MPV buterwa na hypertonic glucose burimo bumwe cyangwa ubwo buryo bwombi, ariko niba aribwo bwa nyuma, noneho kwiyongera kwa MPV byerekana kwangirika.
Ubu bushakashatsi bwerekanye ko guhura cyane na glucose kuri PRP cyangwa platine yuzuye y'amaraso byatumye MPV yiyongera mu minota 15 hamwe na glucose yibanze kuri 25% na 41,6%.
Ubwiyongere bwa platine MPV bushobora guterwa no kwaguka kwa microtubule ikikije ibisubizo bya calcium.Liu n'abandi.Glucose yerekanwe guhuza calcium yinjira binyuze mumashanyarazi ya TRPC6 [6].Igitekerezo cyacu nuko glucose itera kuruhuka kwa microtubule tangles, biganisha ku kwiyongera kwa MPV na sensibilisation ya platel na / cyangwa gukora.Ariko, ukurikije ibisubizo byacu, iki ni igice cyinkuru gusa.Mu bizamini byacu, nta kwibanda munsi ya D25W byatumye MPV yiyongera.Urebye ko tutigeze tugerageza guhura na glucose iri hagati ya 12.5% ​​na 25%, ibisubizo byicyiciro cya 1 byerekana ko hashobora kubaho inzitizi muriki cyiciro cya glucose itera kwiyongera kwa MPV.Ibindi bizamini byo mu cyiciro cya 3 n'icya 4 byerekanye ko glucose ya 20-25% bigaragara ko ari yo mbibi, ariko ntibisobanutse impamvu.
Twabonye kandi igabanuka rya ~ 9% muri MPV nyuma ya centrifugation.Ntabwo byumvikana niba uku kugabanuka kwa MPV guterwa na platine nini kandi yuzuye yafatiwe muri RBC ya centrifuge.Iri genzura rishobora kuba ingenzi kubavuzi kuko rishobora kumvikanisha ko platine ya PRP ari agace gato kandi gake cyane ka WB.
Mu bushakashatsi bwabanje, twerekanye ko gutegura PRP hakoreshejwe uburyo bw'intoki bidahenze [8].Niba glucose ikangurira platine cyangwa PRP, bigatuma irushaho gukora cyane, cyangwa niba PRP ikorwa hamwe na lysate igice, ibi birashobora kongera imbaraga kandi bikagabanya ubuvuzi.Kubwibyo, guhuza PRP hamwe na glucose yibanze cyane birashobora kubahenze kuruta PRP cyangwa glucose yonyine.
Inyigisho yacu ifite ibitagenda neza.Ubwa mbere, dukoresha PRP twabonye muburyo butandukanye.Ibi birashobora gushikana kubisubizo bivuguruzanya.Icya kabiri, ntitwashoboye gukora isesengura ryibinyabuzima kuri buri cyitegererezo cyacu kugirango tumenye neza niba ibikorwa bya platine byarabaye.Turashaka gupima P-selinine, ibintu bya platel 4, igiteranyo cya monocytike ya platine, cyangwa ibindi bimenyetso byerekana ibikorwa bya platel kugirango twumve neza urwego cyangwa ahari alfa granule degranulation, ariko ibi birenze ubushobozi bwubu bushakashatsi.Icya gatatu, ntitwashoboye kwemeza na microscopi ya electron cyangwa ubundi buryo ko kwiyongera kwa MPV muri platine yerekanwe na glucose byatewe ningaruka kuri microtubule.
Imvange ya WB cyangwa PRP hamwe na 25% glucose yiyongereye MPV, byerekana ko ibikorwa bya platel byatangiye, nubwo ubu bushakashatsi buterekanye iterambere ryo guteranya cyangwa gutesha agaciro.Hypertonic glucose ivanze byaviriyemo gutakaza platine, birashoboka ko byerekana ingaruka nziza.Gukora igice cyangwa lysis ya platine birashobora gutera ingirabuzimafatizo nyuma yo gutera inshinge.Ntabwo byumvikana ingaruka zamavuriro izi mpinduka zishobora kuganisha.Ubushakashatsi bwakozwe bwerekanye ibipimo nyabyo byo gukora cyangwa lysis kandi byasuzumye ingaruka zitandukanye z’amavuriro ya hypertonic glucose ivanze na WB cyangwa PRP.
Glucose proliferative therapy ni uburyo bworoshye kandi buhendutse bwo kuvura bushya bugenda bwiyongera kandi bushigikira ubushakashatsi ku mavuriro.Ubu bushakashatsi bwerekana uburyo bwa physiologique, niba byemejwe, byadufasha gusobanukirwa igice cyuburyo bushya bwo kuvura indwara.
Ubumenyi bw’ibinyabuzima n’ubuzima muri kaminuza ya Missouri, Ishuri ry’Ubuvuzi rya Kansas, Umujyi wa Kansas, Amerika
Ibintu byabantu: Abitabiriye ubu bushakashatsi batanze cyangwa ntibatanze uburenganzira.Umuryango mpuzamahanga wubuvuzi bwa selile watanze ICMS-2017-003.Porotokole ikurikira yemejwe kugirango ikoreshwe n’inama ishinzwe isuzuma ry’ikigo mpuzamahanga cy’ubuvuzi bw’utugari: Umutwe: Kubara umusaruro w’ibiyobyabwenge bya plasma ukungahaye kuri platine ushingiye ku mibare fatizo ya CBC.Ibikoko: Abanditsi bose bemeje ko nta nyamaswa cyangwa inyama zagize uruhare muri ubu bushakashatsi.Amakimbirane y'inyungu: Dukurikije ifishi yo kumenyekanisha ICMJE Uniform, abanditsi bose batangaza ibi bikurikira: Amakuru yo kwishyura / serivisi: Abanditsi bose batangaza ko batabonye inkunga y'amafaranga n'umuryango uwo ariwo wose kubikorwa byatanzwe.Isano ry’imari: Abanditsi bose batangaza ko badafite muri iki gihe cyangwa mu myaka itatu ishize bafitanye umubano w’amafaranga n’umuryango uwo ariwo wose ushobora gushishikarira umurimo watanzwe.Ubundi busabane: Abanditsi bose batangaza ko ntayindi mibanire cyangwa ibikorwa bishobora kugira ingaruka kumurimo watanzwe.
Harrison TE, Bowler J, Reeves K n'abandi.(Gicurasi 17, 2022) Ingaruka ya glucose kumubare wa platine nubunini: ingaruka zubuvuzi bushya.Umuti 14 (5): e25081.doi: 10.7759 / gukiza.25081
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