Javascript kuri ubu irahagarikwa muri mushakisha yawe.Bimwe mubiranga uru rubuga ntabwo bizakora niba JavaScript ihagaritswe.
Iyandikishe hamwe nibisobanuro byihariye hamwe nibiyobyabwenge byihariye, kandi tuzahuza amakuru utanga ningingo ziri muri data base yacu hanyuma twohereze imeri ya kopi ya PDF ako kanya.
Ibigize hamwe nibiranga chlorhexidine hydrochloride nanoemulsion nka antibacterial root umuyoboro wamazi wuhira: muri vitro na ex vivo
作者 Abdelmonem R., Younis MK, Hassan DH, El-Sayed Ahmed MAEG, Hassanien E., El-Batuti K., Elfaham A.
Rehab Abdelmonem, 1 Mona K. Younis, 1 Doaa H. Hassan, 1 Mohamed Abd El-Gawad El-Sayed Ahmed, 2 Ehab Hassanein, 3 Kariem El-Batuti, 3 Alaa Elfaham 31 Ubumenyi n'Ikoranabuhanga, Ishami rya Farumasi na Farumasi y'inganda, Kaminuza ya Misr, 6 Ukwakira Umujyi wa Misiri;Ishami rya Microbiology na Immunology, Ishami rya Farumasi, Kaminuza y’ubumenyi n’ikoranabuhanga ya Misr, ku ya 6 Ukwakira, Misiri;Ishami rya Endodontike, kaminuza ya Ain Shams, Cairo, Egiputa Intangiriro n'intego: Chlorine hexidine hydrochloride [Chx.HCl] ifite ibikorwa byinshi bya antibacterial antibacterial, ibikorwa bimara igihe kinini hamwe n'uburozi buke, bityo rero birasabwa ko hashobora kuvomerwa imizi.Icyari kigamijwe muri ubu bushakashatsi kwari ugukoresha ibihimbano bishya Chx.HCl nanoemulsion kugirango yongere imbaraga zinjira, kweza no kurwanya antibacterial ibikorwa bya Chx.HCl no kuyikoresha nk'umuyoboro wamazi.Uburyo: Chx.Tegura igishushanyo mbonera cya pseudo-ternary kugirango werekane sisitemu nziza.Imyiteguro ya nanoemulsion yateguwe yasuzumwe ibirimo ibiyobyabwenge, igihe cya emulisifike, ikwirakwizwa, ingano y’igitonyanga, mu gusohora ibiyobyabwenge bya vitro, ituze rya termodinamike, mu bikorwa bya antibacterial vitro, no mu bushakashatsi bwa vitro bw’ubushakashatsi bwatoranijwe.Igikorwa cyo kwinjira, kweza no kurwanya antibacterial ya Chx.Ibisubizo.Imiterere yatoranijwe yari F6 hamwe na 2% Labrafil, 12% Hagati ya 80 na 6% propylene glycol.Ingano ntoya (12.18 nm), igihe gito cyo kwigana (amasegonda 1.67) no gushonga vuba nyuma yiminota 2.Byagaragaye ko ari sisitemu ya termodinamike / sisitemu ihamye.Ugereranije nubunini busanzwe bwa Chx.Ugereranije nubunini busanzwe bwibintu (2609.56 µm2), 1,6% Chx.HCl nanoemulsion ifite ubuso buto buringaniye bwubuso bwimyanda isigaye (2001.47 µm2).Umwanzuro: Ibigize Nanoemulsion Chx.HCl ifite ubushobozi bwiza bwo gukora isuku nibikorwa bya antibacterial.Ifite igikorwa cyiza cyane cya bagiteri irwanya Enterococcus faecalis, kandi igipimo cyo kugabanuka kwa bagiteri ni kinini cyangwa cyangiritse rwose.Amagambo shingiro: chlorhexidine hydrochloride, nanoemulsion, kuvomera umuyoboro wamazi, kwinjira, ingaruka zo kweza, kuvomera antibacterial.
Nanoemuliyoni, icyiciro cya emulisiyo ifite ubunini bwigitonyanga kiri hagati ya 50-500 nm, byitabweho cyane mumyaka yashize kubera imiterere yihariye.Ibikoresho byiza byogusukura, ntabwo byatewe nubukomere bwamazi, mubihe byinshi usanga bifite uburozi buke no kutagira imikoranire ya electrostatike.2 Nanotehnologiya ifite ubunini buke bwa ultra-nto, ubuso bunini bugereranije ninshi hamwe nibintu bidasanzwe byumubiri nubumara ugereranije nibicuruzwa byinshi, kandi binakingura ibitekerezo bishya mukuvura no gukumira indwara z amenyo.3 Chlorhexidine hydrochloride (Chx.HCl) irashobora gushonga gake mumazi, igashonga gato muri alcool kandi ikagenda yoroha mumucyo.4.5 SH.HCl ifite ibikorwa byinshi bya antibacterial ibikorwa, ibikorwa birebire hamwe n'uburozi buke.Kubera iyi miterere, birasabwa kandi nkibishobora kuvomera umuzi.Ibyiza byingenzi bya Chx.HCl ni cytotoxicity nkeya, nta mpumuro nziza kandi nta buryohe budashimishije.6-9 Ubwoko butandukanye bwa laseri bwakoreshejwe mugutezimbere imizi.Ingaruka ya bagiteri yica lazeri iterwa nuburebure bwumurongo ningufu, kimwe nubushyuhe bwumuriro, butera impinduka murukuta rwa bagiteri, biganisha ku guhinduka kwa osmotic gradient kugeza gupfa.Imikoranire hagati ya lazeri nu muyoboro wamazi wamazi ufungura inzira nshya muri disinfection.10 Ultrasonic ingufu zitanga imirongo myinshi ariko amplitude make. Amadosiye yagenewe kunyeganyega kuri ultrasonic frequency ya 25-30 kHz, irenze imipaka yimyumvire yabantu (> 20 kHz). Amadosiye yagenewe kunyeganyega kuri ultrasonic frequency ya 25-30 kHz, irenze imipaka yimyumvire yabantu (> 20 kHz). Файлы предназначены для колебаний на ульт Канвуковых частотах 25-30 кГц, кот не находятся за пределами слухового воспритияияововка (> 20 кГц). Amadosiye yagenewe kunyeganyega kuri ultrasonic yumurongo wa 25-30 kHz, birenze ubushobozi bwabantu (> 20 kHz).这些 文件 被 设计 成 在 25-30 kHz 的 超声波 频率 下 振荡 ， 这 了>>> (> 20 kHz)。这些 文件 被 设计 成 – 25-30 kHz Файлы рассчитаня на колебания на ульт କାନвуковых частотах 25-30 кГц, что вхходит за пределы слухового вос Пририяияяовека (> 20 кГц). Amadosiye yagenewe kunyeganyega kuri ultrasonic frequency ya 25-30 kHz, irenze imipaka yo kumva kwabantu (> 20 kHz).Bakora muburyo bwo guhindagurika, bashiraho uburyo buranga ipfundo na antinode muburebure bwabo.Ijambo "passive ultrasonic irrigation" (PUI) ni protocole yo kuhira aho nta bikoresho cyangwa inkuta bihura na dosiye cyangwa ibikoresho bya endodontiki.Mugihe cya PUI, ingufu za ultrasound zihererekanwa ziva muri dosiye zinyeganyeza zikavomerera mumazi.Iheruka irashobora gutera sonic itemba hamwe na cavitation ya agent yoza.11 Ukurikije amakuru yavuzwe haruguru, bifatwa nkibikwiye gukoresha nanotehnologiya kugirango dusuzume ibikorwa byinjira no gukora isuku bya Chx.HCl.
Chlorhexidine hydrochloride Chx.HCl yatanzwe neza na Sosiyete ishinzwe imiti y’abarabu ishinzwe imiti (Cairo, Misiri).Labrafil M 1944 CS (oleoylpolyoxy-6-glyceride) yatanzwe na Gattefosse (Padiri mutagatifu, Ubufaransa).Hagati ya 20 (polyoxyethylene (20) sorbitan monolaurate), Hagati ya 80 (polyoxyethylene (80) sorbitan monooleate), aside oleic, propylene glycol yo muri sosiyete ya Gomhorya (Cairo, Misiri)).Gukuramo amenyo adafite amatsiko amenyo yumuti umwe kugirango avurwe igihe cyangwa ortodontique, ishami rya siyanse ya Maxillofacial, ishami ry amenyo, kaminuza ya Ain Shams, Cairo, Misiri.Umuco mwiza wa Enterococcus faecalis (strain ATCC 29212) ikura mumitsi yumutima wubwonko (BHI) (RC CLEANER, IIchung Dental Ltd, Seoul, Koreya).
Ubushakashatsi bwa Chx.HCl mubitangazamakuru bitandukanye (aside oleic, Labrafil M 1944CS, Hagati ya 20, Hagati ya 80, propylene glycol, namazi) byizwe.Umubare munini wa Chx.HCl (50 mg) ushyirwa mumiyoboro ya centrifuge hanyuma hongerwaho 5.0 g yicyiciro giciriritse.Uruvange rwanyeganyezwa muri mixeur ya vortex muminota 15 hanyuma ubikwa mubushyuhe bwicyumba.Nyuma yamasaha 24, pellet yimiti idashobora gushonga muri tube yashizwemo 3000 rpm muminota 5 kugirango ibone ndengakamere isobanutse.Kusanya icyitegererezo gihagije kandi uyunguruze hamwe n-butanol.Ingero zivanze zayungurujwe binyuze mu mpapuro za filteri za Whatman 102 hanyuma zivangwa neza na n-butanol kugirango hamenyekane ibiyobyabwenge byibisubizo byuzuye.Ingero zasesenguwe hamwe na UV spectrophotometero kuri 260 nm hamwe n-butanol nkigenzura.12.13
Igishushanyo mbonera cya pseudo-triple cyubatswe kugirango hamenyekane igipimo nyacyo cya buri kintu gisabwa muburyo bwo kubona ibipimo byiza bya nanoemuliyoni nziza.14 Ihindurwa ryakozwe hifashishijwe amavuta (ni ukuvuga aside oleic na Labrafil M1944CS), surfactants (ni ukuvuga hagati ya 20 na Tween 80) hamwe na surfactant yongeyeho, ni ukuvuga propylene glycol.Ubwa mbere, imvange itandukanye ya surfactants (idafite cosurfactants) hamwe namavuta byateguwe mubipimo bitandukanye (kuva 1: 9 kugeza 9: 1).Iyo imvange yitiriwe amazi (ongeramo amazi atemba), ukurikiranire hafi imvange kuva ibicu kugeza ibicu nkibihe byanyuma.Izi ngingo zanyuma zirangwa ku gishushanyo cya pseudo-eshatu.Inzira yose yasubiwemo kuvanga surfactant na surfactant ya kabiri (Smix) yateguwe mubice 2: 1 na 3: 1 hanyuma ivangwa namavuta yatoranijwe15,16 imwe.
Sisitemu ya Nanoemulsion irimo Chx.HCl yateguwe hifashishijwe Labrafil M 1944 CS nkicyiciro cyamavuta na Hagati ya 80 cyangwa 20 surfactant na propylene glycol nkibindi byongera amazi hanyuma amaherezo, Imbonerahamwe 1. Ibiyobyabwenge byashongeshejwe muri Labrafil M 1944 CS hanyuma amazi ahuriweho na surfactant na surfactant ya kabiri yongerwaho buhoro buhoro no kuvanga buhoro buhoro.Ingano ya surfactant na co-surfactant yongeweho, kimwe nijanisha ryicyiciro cya peteroli gishobora kongerwamo, igenwa hakoreshejwe igishushanyo mbonera cya pseudo-ternary.Imashini itanga ultrasonic (Ultrasonic LC 60 H, Elma, Ubudage) yakoreshejwe kugirango igere ku ntera yifuzwa yo gukwirakwiza granules.Noneho uburinganire.17
Ikizamini cyo gutandukana cyakozwe hifashishijwe ibikoresho byo gusesa (Dr. Schleuniger Pharmaton, Model Diss 6000, Thun, Ubusuwisi) aho ml 1 ya buri myiteguro yongewemo ml 500 y'amazi kuri 37 ± 0.5 ° C.Kwiyoroshya byoroheje byemezwa nicyuma gisanzwe kitagira umuyonga gishonga kizunguruka kuri 50 rpm.Emulsion yavuyemo yagenwe muburyo bugaragara kandi ishyirwa mubikorwa bisobanutse, bisobanutse hamwe nubururu, amata cyangwa igihu.Hitamo formulaire isobanutse yubushakashatsi.18.19
Gukuramo Chx.HCl bivuye muburyo bwiza bwa nanoemuliyoni bushingiye ku gishushanyo mbonera cya pseudo-triple iganisha ku gukora n-butanol ukoresheje tekinoroji ya ultrasonic.Nyuma yo kuyungurura bikwiye, ibivuyemo byasesenguwe kuri spropropotometometricique kumurongo wa 260 nm kubiri muri Chx.HCl.makumyabiri
Kugirango ugerageze kwihesha agaciro, ml 1 ya buri kintu cyongewemo kuri beaker yuzuyemo ml 250 y'amazi yatoboye kandi ikomeza kuri 37 ± 1 ° C hamwe no guhora ikurura 50 rpm.Igihe cyo kwigana-emulisation gifatwa nkigihe preconcentrate ikora imvange imwe nyuma yo kuyungurura.makumyabiri na rimwe
Kugirango isesengura ry'ubunini bw'igitonyanga, koresha mg 50 za formulaire nziza kugeza kuri ml 1000 n'amazi muri flask hanyuma uvange witonze n'intoki.Ikwirakwizwa ry'ubunini bw'igitonyanga ryagenwe hifashishijwe igikoresho cya Malvern Zetasizer 2000 (Malvern Instruments Ltd., Malvern, UK) mu gihe cyo kumenya inyuma ya 173º, ubushyuhe bwa 25ºC, hamwe na index ya 1.330.makumyabiri na kabiri
Muri vitro yo gusesa vitro hakoreshejwe ibikoresho bya USP Type II (paddle) (Dr. Schleuniger Pharmaton, Diss Model 6000) saa kumi nimwe zumugoroba.Amazi yamenetse (ml 500) yabitswe ku bushyuhe bwa 37 ± 0.5 ° C yakoreshejwe nk'uburyo bwo gusesa, naho ml 5 z'ibikoresho byateguwe byongewemo bitonyanga mu buryo bwo gusesa.Hanyuma, mu bihe bitandukanye, hafashwe ml 5 yumuti wo gusesa hanyuma ingano yimiti yarekuwe igaragazwa na spropropotometometricique kuri 254 nm.Ubushakashatsi bwakozwe muri bitatu.makumyabiri na gatatu
Hanyuma, ibipimo bya kinetic ya Chx.HCl irekura muri vitro ivuye muri nanoemuliyoni yateguwe hashingiwe.Zeru, iyambere niyakabiri itondekanya kinetics hamwe na Higuchi diffuzion moderi zageragejwe kugirango zihitemo urutonde rwa kinetic zikwiranye no gusohora Chx.HCl.
Ml 2 ya buri formulaire yabitswe kubushyuhe bwibidukikije mumasaha 48 mbere yo gutandukana kwicyiciro.Icyitegererezo cya ml 1 ya buri Chx.HCl nanoemulsion yakozwe noneho yahinduwe kugeza kuri ml 10 na ml 100 hamwe namazi yatoboye kuri 25 ° C. hanyuma abikwa mumasaha 24.Hanyuma gutandukana kwicyiciro byagaragaye.makumyabiri na rimwe
Hanyuma ingero za ml 2 za buri kintu cyimuriwe mu icupa ryeruye rifite capa ya screw hanyuma ubikwa muri firigo kuri 2 ° C mumasaha 24.Noneho bakuweho babikwa kuri 25 ° C na 40 ° C.Inzira imwe yo gukonjesha-gukonjesha yarakozwe.Ingero zaje kugaragara kugirango zitandukane nicyiciro cyimvura.makumyabiri na rimwe
Icyitegererezo cya ml 5 ya buri Chx.HCl nanoemulsion yimuwe yimuriwe mumirahuri yikirahure ishyirwa muri laboratoire ya laboratoire (Uruganda rwa Shanghai Surgical Instrument Factory Microcentrifuge Model 800, Shanghai, Repubulika yUbushinwa) hanyuma rushyirwa muri 4000 rpm muminota 5.Ingero zaje kugaragara kugirango zitandukane nicyiciro cyimvura.makumyabiri na rimwe
Ubushakashatsi bwose bwemejwe na komite ishinzwe imyitwarire yinzego za kaminuza ya Ain Shams, mu Misiri.Hatoranijwe amenyo 50 adafite amatsiko amenyo yabantu hamwe na apex yashizweho.Amenyo yakuweho yakoreshejwe nyuma yo kubona uruhushya rwanditse rwasinywe numurwayi.Amenyo arimo imashini nini na mandibular incisors na mandibular premolars.Ubuso bw'inyuma bw'imizi bwavuwe hakoreshejwe curette kandi amenyo yose yakorewe sterisizione kuri 0.5% NaOCl mumasaha 24 hanyuma abikwa muri saline sterile kugeza ikoreshejwe.Ikamba ryakuweho hamwe na disiki ya diyama itekanye kandi uburebure bw'iryinyo bwari busanzwe kugera kuri mm 16 kuva kuri apex kugera kumpera ya coronale.24,25 Ukurikije igisubizo cyo kwoza, amenyo agabanyijemo amatsinda akurikira:
(A) Itsinda (n = 24) ingero zogejwe hamwe na Chx.HCl nanoemulsion.Itsinda (I) (n = 12) ryogeje ingero hamwe na ml 5 Chx.HCl nanoemulsion 0,75%.Itsinda (II) (n = 12) ryogeje ingero hamwe na ml 5 ya 1,6% Chx.HCl nanoemulsion..Itsinda rishinzwe kugenzura: (n = 2) ryogejwe hamwe na ml 5 saline idakora.
Batoranijwe 44 badafite amatsiko amenyo yabantu afite imizi.Amenyo arimo imashini nini na mandibular incisors na mandibular premolars.Ubuso bw'inyuma bw'imizi bwavuwe hakoreshejwe curette kandi amenyo yose yakorewe sterisizione kuri 0.5% NaOCl mumasaha 24 hanyuma abikwa muri saline sterile kugeza ikoreshejwe.Amakamba yakuweho hamwe na disiki ya diyama yumutekano kandi uburebure bw amenyo bwari busanzwe kugera kuri mm 16 kuva hejuru kugeza kuri coronale.24,25,29
Gutegura uburyo bwa fayili yingenzi ya fayili 50 ukoresheje uburyo busanzwe.Koresha saline sterile nkuwuhira mugihe cyo kubagwa.Hanyuma, umuyoboro wumuzi wasukuwe hamwe na ml 2 ya 17% EDTA kumunota 1 kugirango ukureho smear.Ubuso bwose bwumuzi, harimo na apical foramen ya buri cyitegererezo, yari itwikiriye amakoti abiri yimisumari (kole ya cyanoacrylate) kugirango wirinde kumeneka.Amenyo ahita ashyirwa muburyo bwa tartar kugirango byoroshye gukora no kumenyekana.29-33 Ingero zahise zikora kuri 121ºC na 15 psi muminota 20.Nyuma yo kuboneza urubyaro, ibyitegererezo byose byatwarwaga kandi bigatunganywa mubihe bidasanzwe hakoreshejwe ibikoresho bya sterile.Imiyoboro yumuzi yandujwe numuco mwiza wa Enterococcus faecalis (strain ATCC 29212) ikura mumitsi yubwonko bwubwonko (BHI) mumasaha 24 kuri 37 ° C.Ukoresheje micropipette sterile, shyiramo ihagarikwa risobanutse rya E. faecalis inoculum mumigezi yateguwe y amenyo yose.Inzitizi zahise zishyirwa muri beaker sterile hanyuma zishyirwa kuri 37 ° C mumasaha 24.31, 34, 35
(A) Itsinda (n = 24) ingero zogejwe hamwe na Chx.HCl nanoemulsion.Ingero zitsinda (I) (n = 12) zogejwe hamwe na ml 5 za Chx.HCl nanoemulsion 0,75%.Itsinda (II) (n = 12) ryogeje ingero hamwe na ml 5 ya Chx.HCl nanoemulsion 1,6%.
Itsinda rishinzwe kugenzura: kugenzura neza, (n = 4) umuyoboro wanduye wanduye washyizwemo saline 5 ml hanyuma ukabikwa nkigenzura ryiza.Igenzura ribi: (n = 4) Ingero ntizatewe inshinge, ni ukuvuga umuyoboro wumuzi ntiwandujwe na E. faecalis, kandi wagumishijwe kuba sterile nkigenzura ribi kugirango hemezwe uburyo bwo kwizerwa no kwizerwa.Koresha ml 5 yo gukaraba igisubizo muri buri sample.Buri cyitegererezo noneho cyogejwe bwa nyuma hamwe na ml 1 ya saline sterile.
Ingano ya 35 sterile impapuro zikoreshwa mugukusanya ingero ziva mumigezi.Impapuro zinjijwe mu muyoboro kugeza ku burebure bw'akazi, hasigara amasegonda 10, hanyuma zoherezwa ku byapa bya agar kugira ngo umenye umubare w’ibice bigize koloni (CFU) kuri buri sahani.Isahani yashizwe kuri 37ºC mu masaha 24 hanyuma isuzumwa muburyo bwo gukura kwa bagiteri.Isahani ibonerana yerekana sterisizione yuzuye.Isahani itagaragara ifatwa nkikura ryiza.Impuzandengo ya CFUs muri zone yo gukura kwa bagiteri kuri buri funguro yaramenyekanye kandi umubare wa CFUs wabazwe.Abacitse ku icumu bapimwa mbere na mbere nibintu bifatika ku byapa bitonyanga.Mubyongeyeho, igikombe cyo gusuka cyakoreshejwe mukubara CFU nkeya, naho ikoreshwa rya 106 ryakoreshejwe mukubara CFU ndende.36.37
Tegura imiyoboro irimo ml 15 ya taged agar medium yabanje kubikwa muri autoclave kumunsi umwe nubushakashatsi.Enterococcus faecalis nubushobozi bwa Gram-positif anaerobic coccus ishobora kubaho kuri pH nyinshi cyane, acide nubushyuhe bwinshi.39 Ingero za bagiteri (Enterococcus faecalis ATCC 29212) zateguwe zivanga selile ziva muri koloni na saline sterile.Ingero za bagiteri zahise zivangwa na saline kugirango zihure na McFarland 0.5, bihwanye na CFU / mL 108.Ingano yicyitegererezo yongeweho yari 10 µl.39 Igipimo cy’imyororokere (McFarland 0.5) 40 cyateguwe usuka 0,6 ml ya 1% (10 g / l) barium chloride dihydrate yumuti muri silinderi 100 yarangije kandi yuzuza ml 100 hamwe na 1% (10 g / l) acide sulfurike.Ibipimo by’imyororokere byashyizwe mu miyoboro imwe n’icyitegererezo cy’umunyu kandi kibikwa ku bushyuhe bw’icyumba amezi 6 mu mwijima kandi bifunzwe kugira ngo birinde guhumeka.Fungura umupfundikizo wibiryo bya Petri birimo ubusa hanyuma usukemo icyitegererezo hagati yisahani.Niba agar imaze gukomera rwose, hindura isahani hanyuma ushire kuri 37 ° C mumasaha 24.
Amakuru yose yakusanyirijwe, ateganijwe kandi akorerwa isesengura mibare.Isesengura mibare ryakozwe hifashishijwe IBM® SPSS® Statistical Version 17 ya Windows (SPSS Inc, IBM Corporation, Armonk, NY, USA).
Gukemura kwa Chx.HCl mubice bitandukanye byamavuta, ibisubizo bya surfactant, ibisubizo bifatanije, namazi byizwe.Chx.Hcl ifite solubilité nyinshi muri Labrafil M hamwe nubushyuhe buke muri acide oleic.Kwiyongera kwinshi kwibiyobyabwenge mugice cyamavuta nibyingenzi kuri nanoemuliyoni kuko nanoemuliyumu ishoboye kugumisha imiti muburyo bwashonze, bivuze ko gukomera kwinshi mumiti bivamo amavuta make mubitera bityo ibiyobyabwenge bike.gupakira Umubare munini wa surfactant hamwe na co-surfactant urasabwa kwigana ibitonyanga byamavuta.
Igishushanyo mbonera cya pseudo-triple cyubatswe kugirango hasobanurwe uturere twa nanoemuliyoni no guhuza neza amavuta yatoranijwe, surfactants, hamwe nandi mashanyarazi (Labrafil M, Hagati ya 80, Hagati ya 20, na propylene glycol).Chx.Kubwibyo, sisitemu ya oleic aside yakuwe muri ubu bushakashatsi.Ibindi byateguwe byateguwe hakoreshejwe 1: 9 ivanze ryamavuta na surfactant.urwego rwa pH nimbaraga za ionic, ubwo rero surfactants zatoranijwe.
Ibisobanuro byose byateguwe byari bisobanutse usibye Sisitemu F2, yagaragaraga nk'igicu bityo ikaba itavanyweho mu yandi masomo yo gusuzuma.
Uburyo bwiza bwa nanoemulsion bugomba gushobora gutatana burundu kandi vuba mugihe bivanze nubwitonzi bworoheje.Chx.HCl nanoemulsion yerekanaga igihe gito cyo kwigana, kuva kumasegonda 1.67 kugeza 12.33.Hagati ya 80 ifite igihe gito cyo kwigana.Ibi birashobora gusobanurwa nubushobozi buhanitse bwo hagati ya Tween 80. Igihe cyo kwigira-emulisiyasi cyiyongera hamwe no kwiyongera kwa surfactant, bishobora guterwa no kwiyongera kwijimye rya sisitemu ikorwa na surfactant.
Ingano yigitonyanga ya emulsiyo igena igipimo nintera yo gusohora ibiyobyabwenge.Ingano ntoya ya emulsiyo itanga igihe gito cyo kwigana hamwe nubuso bunini bwo kwinjiza ibiyobyabwenge.Impuzandengo yigitonyanga cyibipimo byatoranijwe bya Chx.HCl nanoemulsion yari 711 ± 0.44, 587 ± 15.3, 10.97 ± 0.11, 16.43 ± 4.55, na 12.18 ± 2.48, naho PDI yari 0 .76, 0.19, 0.61, 0.47 na 0.76 kuri F1, F2., F3 na 0.16 bikurikiranye F4, F5 na F6.Imiterere irimo Tween 80 nka surfactant yerekanaga spherulite nto.Ibi birashobora guterwa nimbaraga zayo zo hejuru.Agaciro gato ka PDI yerekana ingano ya sisitemu igabanijwe.Iyi formulaire ifite isura nziza kuko radiyo yigitonyanga ni ntoya kurenza optique yumurambararo wa optique yumucyo ugaragara (390-750 nm) aho urumuri rudasanzwe.41
Ku mutini.2 yerekana ijanisha rya Chx.HCl yasohotse muburyo bwateguwe.Kurekura burundu ibiyobyabwenge bivuye muburyo bwateguwe bwa Chx.HCl nanoemulsion kuva muminota 2 kugeza 7.Byagaragaye ko igipimo kinini cyo kurekura ibiyobyabwenge cyabonetse ku bijyanye na Chx.HCl F6 nanoemulsion (2 min), ishobora guterwa no kuba hagati ya 80, yerekanaga urwego rwo hejuru rwa emulisation, hamwe na nanoemuliyoni.itanga ubuso bunini bwo kurekura ibiyobyabwenge, bigatuma ibiciro byiyongera byibiyobyabwenge.Muri icyo gihe, imiterere ya solubile ya propylene glycol ituma ubwinshi bwa hydrophilique surfactants zishonga mumavuta.40
Chx.HCl irekura muri vitro byagaragaye ko ikurikiza gahunda itandukanye, kandi nta tegeko risobanutse neza rishobora kwerekana irekurwa ryibiyobyabwenge bivuye muburyo butandukanye bwa nanoemulsion.Kurekura kinetic yibiyobyabwenge bya F4 nubwa mbere gahunda ya kinetics, bivuze ko irekurwa ukurikije umubare wibiyobyabwenge bisigaye muri bo.42 Kurekura kwa kinetic y’ibindi biyobyabwenge byari bihuye n’ikwirakwizwa rya Higuasha, ryerekanaga ko ingano y’ibiyobyabwenge yasohotse yagereranyaga n’umuzi wa kare w’ibiyobyabwenge byose hamwe n’ibiyobyabwenge muri nanoemulsion.42
Ibyatoranijwe byatoranijwe byakorewe itandukanyirizo rya termodinamike mugupima ibibazo ukoresheje ubushyuhe bukonje, centrifugation hamwe na freeze-thaw cycle.Byagaragaye ko imiterere F3 na F4 yerekanaga imvura yibiyobyabwenge nyuma yo gukonja, mugihe F1 yerekanaga umubyimba (gelling).F5 na F6 batsinze centrifugation ikomeza, ikizamini cyo gushyushya-gukonjesha no gukonjesha.Nanoemuliyumu ni sisitemu ihamye ya termodinamike ikozwe mubice bimwe byamavuta, surfactant namazi nta gutandukanya icyiciro, emulisation cyangwa guturika.Nubushyuhe bwumuriro butandukanya nanoemuliyoni na emulsiyo, ihagaze neza kandi amaherezo izatandukana mubice.19 F3 yerekanye ubunini bunini (587 nm) kurusha ubundi buryo, bushobora gusobanura gutandukanya icyiciro n’imvura igwa mu bizamini bya termodinamike.F4 irimo Hagati ya 80 kandi nta s-surfactant yerekanye imvura igwa, ibi birashobora kwerekana ko ari ngombwa gukoresha propylene glycol na Tween 80 kugirango tunonosore imitekerereze ya nanoemulsion.F1 irimo Tween 20 idafite surfactant yongeyeho kwerekana umubyimba (gelling), ibyo bikaba byiyongera mubwiza bwa gel cyangwa imbaraga bitewe no guteranya ibitonyanga.
Ibisubizo bihamye byerekana akamaro ko kuba hari ikindi kintu cyitwa propylene glycol cyongera imbaraga kugirango ikwirakwize ibice kandi birinde ibiyobyabwenge.43 F6 niyo yari nziza cyane bitewe nubunini buke (12.18 nm), igihe gito cyo kwigana (amasegonda 1.67) nigipimo cyihuta nyuma yiminota 2.Byagaragaye ko ari sisitemu ya termodinamike / ihamye kumubiri bityo ikaba yaratoranijwe kugirango ikore ubushakashatsi.
Kunanirwa nyuma yo kuvura imiyoboro y'amazi bigenda byiyongera, bivuze ko abarwayi bafite ibyago byinshi byo kwandura indwara zikomeye.44,45 Biofilm igomba gukurwaho mugihe cyo kwanduza no kuzuza imiyoboro yumuzi.46.47 Kubera ubwinshi bwimikorere yumuyoboro wumuzi, biragoye kuvanaho burundu imiyoboro ya bagiteri ukoresheje ibikoresho gusa no kuhira.48 Imikorere yumuti wogeje umuzi biterwa no kwinjirira mumazi muri DT hamwe nigihe cyo guhura na bagiteri.49 Kubwibyo, uburyo bushya bwo gushinga imizi neza bwarageragejwe kandi burageragezwa.Kwoza bisanzwe ntibikuraho burundu E. faecalis kubera kwinjira kwa DT.50.
Impuzandengo yo gukora isuku ya nanoemulsion yogeje yari 2001.47 µm2, naho impuzandengo yingirakamaro yimfashanyo yo kwoza yari 2609.56 µm.Ikigereranyo cyo hagati yo gukaraba nanoemulsion no gukaraba ingano isanzwe yari 608.09 µm2. Hariho itandukaniro rifite imibare ikomeye cyane (P <0.001) hagati yuhira nanoemulsion nuhira bisanzwe byingirakamaro hamwe na (P-agaciro 0.00052). Hariho itandukaniro rifite imibare ikomeye cyane (P <0.001) hagati yuhira nanoemulsion nuhira bisanzwe byingirakamaro hamwe na (P-agaciro 0.00052). Между григационными растворами нано Мюльсии и кригаганыными рацворами с башныным размером частиц наблюдалась статистически високозначимая (P <0,001) разница (значение P 0,00052). Hariho imibare ihambaye cyane (P <0.001) itandukaniro (P agaciro 0.00052) hagati yuhira nanoemulsion nuhira ibice bisanzwe.纳米 乳液 冲洗 剂 和 正常 粒径 冲洗 <<<<<P <0.001） （P 值 0.00052）。纳米 乳液 冲洗 剂 和 正常 粒径 冲洗 <<<<<P <0.001） （P 值 0.00052）。 Между ополаскивеелем с нано Мульссией и ополаскиватем с с Hariho imibare itandukanye cyane (P <0.0001) hagati yo koga kwa nanoemulsion hamwe nubunini busanzwe bwo kwoza (P agaciro 0.00052).Nanoemuliyoni yerekanaga itandukaniro rinini cyane ugereranije nubunini busanzwe bwibikoresho, byerekana ubuso buke busigara hejuru yimyanda, ni ukuvuga ibikoresho bya nanoemuliyoni byari bifite ubushobozi bwiza bwo gukora isuku, nkuko bigaragara ku gishushanyo cya 3.
Igicapo 3. Kugereranya imikorere yisuku yimfashanyo yoza: (A) na laser ya Nano CHX ikora, (B) hamwe na laser ya CHX ikora, (C) hamwe na PUI Nano CHX, (D) idafite ibikorwa bya Nano CHX, (E) idafite ibikorwa bya CHX, na (F)) CHX PUI.
Impuzandengo yubuso bwa Chx isigaye.HCl 1,6% ibice byari 2320.36 µm2, naho ubuso bwubuso bwa Chx.HCl 2% bwari 2949.85 µm2. Hariho itandukaniro rinini cyane (P <0.001) hagati yubushuhe bwinshi bwamazi ya nanoemulsion hamwe nubunini busanzwe bwamazi (P-agaciro 0.00000). Hariho itandukaniro rinini cyane (P <0.001) hagati yubushuhe bwinshi bwamazi ya nanoemulsion hamwe nubunini busanzwe bwamazi (P-agaciro 0.00000). Наблюдалась статистически высокозначимая (P <0,001) разница между более высокой мирацией нано Прал чных чригаганыных растворов и иригригационными растворами с ча пы Hariho itandukaniro rifite imibare ikomeye cyane (P <0.001) hagati yubushuhe bwinshi bwamazi ya nanoemulsion hamwe nubunini busanzwe bwamazi (P agaciro 0.00000).<浓度 的 纳米 乳液 乳液 剂 <<<<<P <0.001） （P 值 0.00000）。较高 浓度 的 纳米 乳液 冲洗 剂 与 <<<<<<P <0.001） （P 0 0.0 Наблюдалась Статистически очень значимая разница (P <0,001) между более всок зим мыри ополаскивателя с нано Мурсисией и ополаскиветеля с черныным размером частиц (значение P 0,00000). Hariho imibare itandukanye cyane (P <0.001) hagati yubushuhe bwinshi bwa nanoemulsion rinse hamwe nubunini busanzwe bwo kwoza (P agaciro 0.00000).Nubwo kwibumbira hamwe kwuhira kwa nanoemuliyoni kwari munsi ugereranije nubunini busanzwe bwuhira, ubwo bwiyongere bwo hasi bwagize akamaro kanini mugukuraho imyanda kandi bugira ingaruka nziza mugusukura imizi.
PUI yari ifite imibare itandukanye cyane (p <0.001) mugihe ugereranije nubundi buryo bwo gukora. PUI yari ifite imibare itandukanye cyane (p <0.001) mugihe ugereranije nubundi buryo bwo gukora. PUI имел статистически высокозначимую разницу (p <0,001) по сравнению с друг матоода активи. PUI yari ifite itandukaniro rinini cyane (p <0.001) ugereranije nubundi buryo bwo gukora.与 其他 激活 方法 相比 UI PUI 具有 统计学 上 非常 显 着 的 p (p <0.001)。与 其他 激活 方法 相比 UI PUI 具有 统计学 上 非常 显 着 的 p (p <0.001)。 П сравнению с друг мин методами активации PUI imел статистически очень значимую разницу (p <0,001). Ugereranije nubundi buryo bwo gukora, PUI yari ifite imibare itandukanye cyane (p <0.001).Hamwe nogukora kwa ISP, impuzandengo yubuso bwubuso bwimyanda yari 1695.31 µm2. Itandukaniro riri hagati ya PUI na Laser ryari 987.89929 ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p-agaciro 0.00000). Itandukaniro riri hagati ya PUI na Laser ryari 987.89929 ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p-agaciro 0.00000). Средняя разница между PUI na Laser составила 987,89929, демонстрируя высокостатистически значимую (P <0,001) разницу с (p-значение 0,00000). Itandukaniro riri hagati ya PUI na Laser ryari 987.89929, ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro kuva (p-agaciro 0.00000). PUI er Laser 之间 的 平均 差异 为 987.89929 ， 显示 出 高度 统计学 显 着 性 (P <0.001) 差异 (p 值 0.00000)。PUI 和 Laser Средняя разница между PUI na Laser составила 987,89929, что свидетельствует о визокой статистической значимости (P <0,001) разницы (p-значение 0,00000). Itandukaniro riri hagati ya PUI na Laser ryari 987.89929, ryerekana ubusobanuro buhanitse bwibarurishamibare (P <0.001) itandukaniro (p-agaciro 0.00000). Itandukaniro riri hagati ya PUI kandi nta activation ryabaye 712.40643 ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na p-agaciro ka 0.00098) .Ikoreshwa ryaba Laser activation cyangwa ntagikorwa cyakozwe ntabwo cyari gifite imibare (P> 0.05) itandukanye na P-agaciro ka 0.451211. Itandukaniro riri hagati ya PUI kandi nta activation yari 712.40643 yerekana itandukaniro rinini cyane (P <0.001) itandukaniro na p-agaciro ka 0.00098).a P-agaciro ka 0.451211. Средняя разница между PUI и отсутствием активяи составила 712,40643, демонстрируя высокостатистически значимую (P <0,001) разницу с p-значением 0,00098). Itandukaniro riri hagati ya PUI no kutagira ibikorwa byari 712.40643, byerekana itandukaniro rinini cyane (P <0.001) itandukaniro na p-agaciro ka 0.00098).P-agaciro 0.451211. PUI 和 未 激活 之间 的 平均 差异 为 712.40643 ， 显示 高度 统计学 显 着 性 (P <0.001) ， p 值为 0.00098)。PUI Средняя разница между PUI и интактиваей составила 712,40643, что свидетельствует о высокой статистической значимости разницы (P <0,001, p-значение 0,00098). Itandukaniro riri hagati ya PUI no kudakora byari 712.40643, byerekana akamaro gakomeye k’imibare itandukanye (P <0.001, p-agaciro 0.00098).使用 激光 激活 或 不 激活 在 统计学 P P (P> 0.05) P 值为 0.451211。使用 激光 激活 或 不 激活 在 统计学 P P (P> 0.05) P 值为 0.451211。 Статистически значимой разницы (P> 0,05) с лазерной активией или не не не было со значением P 0,451211. Nta tandukanyirizo rifite imibare (P> 0.05) hamwe cyangwa idafite lazeri ifite P ifite agaciro ka 0.451211.Impuzandengo yubuso bwibice bisigaye nyuma yo gukora laser yari 2683.21 µm2.Impuzandengo yubuso bwibice bisigaye nta gukora byari 2407.72 µm2.Ugereranije no gukora laser cyangwa nta activation, PUI yari ifite imibare mito mito igereranije, ni ukuvuga imbaraga nziza zo gukora isuku.
Impuzandengo yo gukora isuku ya nanoemulsion yogeje yari 2001.47 µm2, naho impuzandengo yingirakamaro yimfashanyo yo kwoza yari 2609.56 µm.Ikigereranyo cyo hagati yo gukaraba nanoemulsion no gukaraba ingano isanzwe yari 608.09 µm2. Hariho imibare ihambaye cyane (P <0.001) hagati yuhira nanoemulsion nuhira ningero zisanzwe zingana na (P-agaciro 0.00052). Hariho imibare ihambaye cyane (P <0.001) hagati yuhira nanoemulsion nuhira ningero zisanzwe zingana na (P-agaciro 0.00052). Между григационными растворами нано Мульссии һәм матригационными растворами с башныным размером частиц б мат статистически визокозначимая (P <0,001) разница (значение P 0,00052). Hariho imibare ihambaye cyane (P <0.001) itandukaniro (P agaciro 0.00052) hagati yuhira nanoemulsion nuhira ibice bisanzwe.纳米 乳液 冲洗 剂 与 正常 粒径 冲洗 <<<<<P <0.001） （P 值 0.00052）。 P <0.001） （P 值 0.00052）。 Между ополаскивеелем с нано Мульссией и ополаскиватем с с Hariho imibare itandukanye cyane (P <0.0001) hagati yo koga kwa nanoemulsion hamwe nubunini busanzwe bwo kwoza (P agaciro 0.00052).Ugereranije nubunini busanzwe bwibintu, nanoemuliyoni ifite itandukaniro rinini cyane mubibare, byerekana ubuso buke busigara busigara hejuru yubutaka, ni ukuvuga ibikoresho bya Nanoemulsion bifite ubushobozi bwo gukora isuku nkuko bigaragara mumashusho 3.
Impuzandengo yubuso bwa Chx isigaye.HCl 1,6% ibice byari 2320.36 µm2, naho ubuso bwubuso bwa Chx.HCl 2% bwari 2949.85 µm2. Hariho imibare ihambaye cyane (P <0.001) hagati yubushuhe bwinshi bwamazi ya nanoemulsion hamwe namazi asanzwe yuhira (P-agaciro 0.00000). Hariho itandukaniro rinini cyane (P <0.001) hagati yubushuhe bwinshi bwamazi ya nanoemulsion hamwe nubunini busanzwe bwamazi (P-agaciro 0.00000). Имелась статистически высокодостоверная (P <0,001) разница между более высокой мирацией нано Прул Кнххгригационных средств и иригиганыными растворами с савныны размером частиц (значение P 0,00) Hariho itandukaniro rifite imibare (P <0.001) hagati yubushuhe bwinshi bwamazi ya nanoemulsion hamwe nubunini busanzwe bwamazi (P agaciro 0.00000).<浓度 的 纳米 乳液 乳液 剂 <<<<<P <0.001） （P 值 0.00000）。<浓度 的 纳米 乳液 冲洗 剂 与 <<<<P <0.001） （P000 Наблюдалась статистически высокозначимая разница (P <0,001) между более высок мим мәми ополаскивателя с нано Мурсисией и ополаскиветелем с ски чныным размером частиц (значение P 0,00000). Hariho itandukaniro rinini cyane mubarurishamibare (P <0.001) hagati yubushuhe bwinshi bwa nanoemulsion rinse hamwe nubunini busanzwe bwo kwoza (P agaciro 0.00000).Nubwo kwibumbira hamwe kwuhira kwa nanoemuliyoni kwari munsi ugereranije nubunini busanzwe bwuhira, ubwo bwiyongere bwo hasi bwagize akamaro kanini mugukuraho imyanda kandi bugira ingaruka nziza mugusukura imizi.
PUI yari ifite imibare itandukanye cyane (p <0.001) mugihe ugereranije nubundi buryo bwo gukora. PUI yari ifite imibare itandukanye cyane (p <0.001) mugihe ugereranije nubundi buryo bwo gukora. PUI imел статистически высокую значимую разницу (p <0,001) по сравнению с друг мимодами активяи. PUI yari ifite itandukaniro rinini cyane (p <0.001) ugereranije nubundi buryo bwo gukora.与 其他 激活 方法 相比 UI PUI 具有 统计学 上 的 显 着 p (p <0.001)。 Ugereranije nubundi buryo bwo gukora, PUI ifite itandukaniro rinini cyane (p <0.001). PUI статистически значимо отличался (p <0,001) по сравнению с друг матоодами активии. PUI yari itandukanye cyane (p <0.001) ugereranije nubundi buryo bwo gukora.Mugihe cyo gukora PUI, impuzandengo yubuso bwimyanda isigaye yari 1695.31 μm2. Itandukaniro riri hagati ya PUI na Laser ryari 987.89929 ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p-agaciro 0.00000) .Itandukaniro riri hagati ya PUI no kutagira ibikorwa byari 712.40643 ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p-agaciro 0.00098) .Ikoreshwa ryibikorwa bya Laser cyangwa nta bikorwa byari bifite imibare itandukanye (P> 0.05). Itandukaniro riri hagati ya PUI na Laser ryari 987.89929 ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p-agaciro 0.00000).Itandukaniro riri hagati ya PUI kandi nta activation yari 712.40643 yerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p -agaciro 0.00098).Gukoresha ibikorwa bya Laser cyangwa nta activation byari bifite ntanumwe muburyo bugaragara (P> 0.05) bitandukanye na (P-agaciro 0.451211). Средняя разница между PUI и лазером составила 987,89929, демонстрируя высокостатистически значимую (P <0,001) разницу с (p-значение 0,00000). Itandukaniro riri hagati ya PUI na laser ryari 987.89929, ryerekana itandukaniro rinini cyane (P <0.001) itandukaniro na (p-agaciro 0.00000). - значение 0,00098). - agaciro 0.00098).Gukoresha lazeri cyangwa nta activation byari bifite itandukaniro rinini cyane (P> 0.05) hamwe na (P-agaciro 0.451211). PUI 和 激光 之间 的 平均 差异 为 987.89929 ， 与 (p 值 0.00000) 差异 具有 高度 统计学 P (P <0.001)。 Ikigereranyo cyo hagati ya PUI na laser ni 987.89929, kandi itandukaniro (p 值 0.00000) rifite akamaro gakomeye mu mibare (P <0.001). Средняя разница между PUI и лазером составила 987,89929, что было визоко статистически значимым (P <0,001) с (значение p 0,00000). Itandukaniro riri hagati ya PUI na laser ryari 987.89929, ryari rifite imibare ikomeye cyane (P <0.001) hamwe na (p agaciro 0.00000). PUI 与 未 激活 之间 的 平均 差异 为 712.40643 ， 与 (p) 差异 具有 高度 统计学 意义 (P <0.001) - 值 0.00098)。 Ikigereranyo cyo hagati ya PUI no kudakora ni 712.40643, kandi itandukaniro (p) rifite ubusobanuro buhanitse bwibarurishamibare (P <0.001) - agaciro 0.00098. Средняя разница между PUI и интакитасей составила 712,40643, что было высоко статистически значимым с разницей (p) (P <0,001 - значение 0,00098). Itandukaniro riri hagati ya PUI no kudakora ni 712.40643, ryari rifite imibare ikomeye cyane itandukanye (p) (P <0.001 - agaciro 0.00098).使用 激光 激活 或 不 激活 没有 显 P (P> 0.05) 与 (P 值 0.451211)。 Nta tandukanyirizo rishingiye ku mibare ryari hagati yo gukora laser no kudakora (P> 0.05) na (P 值 0.451211). Не было статистически значимой разницы (P> 0,05) по сравнению с (значение P 0,451211) с лазерной активяей или бар нее. Nta tandukanyirizo rishingiye ku mibare (P> 0.05) ugereranije na (P agaciro 0.451211) hamwe na lazeri cyangwa idafite.Impuzandengo yubuso bwibice bisigaye mugihe cyo gukora laser byari 2683.21 μm2.Impuzandengo yubuso bwibice bisigaye nta gukora byari 2407.72 μm2.Ugereranije no gukora laser cyangwa nta activation, PUI ifite imibare mito mito igereranije yubuso bwa chip, ni ukuvuga ubushobozi bwiza bwo gukora isuku.
Ingaruka ngereranyo yo kwoza nanoemuliyoni mugukuraho imyanda yari hejuru cyane mubaruta iy'ubunini busanzwe bwogejwe.Chx.HCl 1,6%, PUI 1938.77 µm2, 2510.96 µm2 hamwe na laser.Hatabayeho gukora, impuzandengo ni 2511.34 µm2.Iyo 2% Chx.HCl yakoreshejwe kandi igakoreshwa na laser, ibisubizo byari bibi cyane kandi umubare wimyanda wari mwinshi.Ibisubizo bimwe byabonetse mugihe 0,75% Chx.HCl itakoraga.Ikigaragara ni uko ibisubizo byiza byabonetse hakoreshejwe imbaraga nyinshi zo gufashwa muri nanoemulsion.PUI yagize akamaro kanini mugikorwa cyo kuhira no gusukura imyanda, nkuko bigaragara ku gishushanyo cya 3A-F)).
Nkuko bigaragara mu mbonerahamwe ya 2, Chx.HCl nanoemuliyoni yakoze neza kurusha ingano yubunini busanzwe mubijyanye na mikorobe ifatika kandi ifite isano ryiza hamwe no kwinjiza no guhanagura ukurikije ibipimo bikurikira: ubunini, kwibanda kumashanyarazi hamwe nuburyo bwo gukora.
Indwara ya bagiteri irashobora gusenywa burundu ukoresheje imbaraga nyinshi zo gufasha.Ndetse hamwe na PUI ikora, 0,75% Chx.HCl yagize ingaruka mbi ya antibacterial.Gukora lazeri bigira ingaruka mbi kuri nano-emulion.Nkuko bigaragara mubisubizo byose byabanjirije iki, gukoresha laser bigabanya imikorere ya Chx.HCl 0,75% nanoemulsion, aho CFU ya nanoChx.HCl 0,75% ni 195, nigiciro kinini cyane, byerekana ko reagent kuriyi concentration igereranywa no gukora laser.Lazeri ya Diode ifotora, bityo urumuri cyangwa ubushyuhe birashobora gutuma nanoemuliyoni itakaza ingaruka za antibacterial.Igisubizo cyo kwibanda cyane ni ugusenya burundu bagiteri.Nano Chx.Turashobora kwanzura ko ibikoresho bya nanoemuliyoni hamwe nibitekerezo byinshi bifite antibacterial nziza.
Muri iki gikorwa, Hx.HCl nanoemuliyoni yateguwe hifashishijwe amavuta abiri atandukanye, surfactants ebyiri hamwe na co-surfactant, formulaire nziza (F6) ifite ingano ntoya, igihe gito cya emulisation nigipimo kinini cyo guseswa).Mubyongeyeho, (F6) yageragejwe kuri thermodynamic / stabilite physique.Muri Chx.Byongeye kandi, ubushakashatsi bwa antibacterial ya Chx.HCl nanoemulsion yerekanye kurandura burundu bagiteri.Ibisubizo byemeje ibi.Chx.HCl nanoemulsion irashobora gufatwa nkamazi meza yo gukaraba.
Turashimira cyane abakozi ba laboratoire yubushakashatsi ya Misr University of Science and Technology kubwinkunga ikomeye.