Javascript yakadzimwa mubrowser yako parizvino.Zvimwe zvewebhusaiti ino hazvishande kana JavaScript ikavharwa.
Bhalisa nemashoko ako chaiwo uye mushonga wekufarira, uye isu tichafananidza ruzivo rwaunopa nezvinyorwa mune yedu yakakura dhatabhesi uye email iwe kopi yePDF nekukasira.
Kuumbwa uye hunhu hwe chlorhexidine hydrochloride nanoemulsion seanovimbisa antibacterial mudzi canal irrigant: in vitro uye ex vivo zvidzidzo.
作者 Abdelmonem R., Younis MK, Hassan DH, El-Sayed Ahmed MAEG, Hassanien E., El-Batuti K., Elfaham A.
Rehab Abdelmonem, 1 Mona K. Younis, 1 Doaa H. Hassan, 1 Mohamed Abd El-Gawad El-Sayed Ahmed, 2 Ehab Hassanein, 3 Kariem El-Batuti, 3 Alaa Elfaham 31 Sainzi neTekinoroji, Faculty of Pharmacy and Industrial Pharmacy, Misr City, Egypt;2 Dhipatimendi reMicrobiology uye Immunology, Faculty of Pharmacy, Misr University of Science and Technology, Gumiguru 6, Egypt;3 Dhipatimendi reEndodontics, Ain Shams University, Cairo, Egypt Nhanganyaya uye Chinangwa: Chlorine hexidine hydrochloride [Chx.HCl] ine yakakura spectrum antibacterial basa, kuita kwenguva refu uye yakaderera toxicity, saka inokurudzirwa sechinhu chinogona midzi canal irrigant.Chinangwa chechidzidzo ichi chaiva chekushandisa shanduro itsva Chx.HCl nanoemulsion kuti iwedzere simba rinopinda, kuchenesa uye antibacterial action yeChx.HCl uye kuishandisa semudziyo wekudiridza.Nzira: Chx.HCl nanoemulsions dzakagadzirirwa kushandisa mafuta maviri akasiyana: oleic acid uye Labrafil M1944CS, maviri surfactants, Tween 20 uye Tween 80, uye co-surfactant, propylene glycol.Ronga pseudo-ternary phase diagraph kuratidza iyo yakakwana system.Iyo yakagadzirirwa nanoemulsion formulations yakaongororwa kuti ione zvinodhaka, emulsification nguva, dispersibility, droplet size, in vitro drug release, thermodynamic stability, in vitro antibacterial activity, uye in vitro zvidzidzo zvezvakasarudzwa zvakagadzirirwa.Kupinda, kuchenesa uye antibacterial action yeChx.HCl 0.75% uye 1.6% nanoemulsion yakaenzaniswa neyakajairwa particle size semudzi wemvura inodiridza.Results.Iyo yakagadzirwa yakasarudzwa yaive F6 ine 2% Labrafil, 12% Pakati pe80 uye 6% propylene glycol.Diki particle size (12.18 nm), pfupi emulsification nguva (1.67 seconds) uye kukurumidza kupera mushure memaminitsi maviri.Yakawanikwa iine thermodynamically / physically stable system.Kuenzaniswa neyakajairwa Chx.HCl particle size, iyo yakakwirira yeChx.HCl 1.6% nanoemulsion yakaratidza kupinda zviri nani nekuda kwediki diki.Zvichienzaniswa neyakajairwa particle size material (2609.56 µm2), iyo 1.6% Chx.HCl nanoemulsion ine diki paavhareji nzvimbo yemarara asara (2001.47 µm2).Mhedziso: Nanoemulsion composition Chx.HCl ine zvirinani kuchenesa kugona uye antibacterial chiito.Iine bactericidal inoshanda zvakanyanya kurwisa Enterococcus faecalis, uye hutachiona hwesero rekutapudza mwero wakakwira kana kuparadzwa zvachose.Mashoko akakosha: chlorhexidine hydrochloride, nanoemulsion, mudzi canal irrigant, kupinda, kuchenesa maitiro, antibacterial irrigant.
Nanoemulsions, kirasi yema emulsions ane madonhwe saizi muhupamhi hwe50-500 nm, yakagamuchira kutariswa kwakawanda mumakore achangopfuura nekuda kwehunhu hwavo hwakasiyana.Yakanaka yekuchenesa zvivakwa, haina kukanganiswa nekuoma kwemvura, kazhinji ine yakaderera toxicity uye kusavapo kwe electrostatic kupindirana.2 Nanotechnology ine hukuru-hudiki diki saizi, nzvimbo yakakura kusvika kuhuwandu huyero uye yakasarudzika yemuviri nemakemikari zvinhu zvichienzaniswa neakawanda zvigadzirwa, uye zvakare inovhura maonero matsva mukurapa nekudzivirira hutachiona hwemazino.3 Chlorhexidine hydrochloride (Chx.HCl) inonyungudika zvishoma mumvura, inonyungudika zvishoma mudoro uye zvishoma nezvishoma inonyungudika muchiedza.4.5 SH.HCl ine yakafara spectrum antibacterial chiito, kwenguva refu chiito uye yakaderera muchetura.Nekuda kwezvivakwa izvi, inokurudzirwa zvakare seinogoneka mudzi canal irrigant.Izvo zvakakosha zveChx.HCl zvakaderera cytotoxicity, hapana kunhuwa uye hapana kunakidza kuravira.6-9 Mhando dzinoverengeka dzemalaser dzakashandiswa kuvandudza midzi canal disinfection.Iyo bactericidal mhedzisiro ye lasers inoenderana newavelength uye simba, pamwe nekuratidzwa kwekupisa, izvo zvinokonzeresa shanduko mubhakitiriya sero madziro, izvo zvinotungamira kune shanduko yeosmotic gradient kusvika kurufu rwesero.Iko kudyidzana pakati pemalaser uye midzi canal irrigators inovhura mahorizons matsva mu pulp disinfection.10 Ultrasonic simba inogadzira yakakwirira frequency asi yakaderera amplitudes. Iwo mafaera akagadzirirwa kutenderera pa ultrasonic frequencies ye 25–30 kHz, ayo ari kupfuura muganhu wekuona kwekunzwa kwevanhu (> 20 kHz). Iwo mafaera akagadzirirwa kutenderera pa ultrasonic frequencies ye 25–30 kHz, ayo ari kupfuura muganhu wekuona kwekunzwa kwevanhu (> 20 kHz). 25–30 кГц, которые находятся за пределами слухового восприкажение пределами слухового воспория восприя осправи. Iwo mafaera akagadzirwa kuti aite zvekuzunguza pa ultrasonic frequencies ye 25-30 kHz, iyo iri kupfuura huwandu hwekunzwa kwevanhu (> 20 kHz).這些文件被设计成在25–30 kHz 的超声波频率下振荡，這超出了人类听觉感知的极限(>20 kHz).這些文件被设计成在 25–30 kHz Файлы рассчитаны на колебания на ультразвуковых частотах 25–30 кГц, что выходит за пределы слухового восприятия человека (>). Iwo mafaera akagadzirirwa vibrations pa ultrasonic frequencies ye 25-30 kHz, iyo inodarika miganhu yekunzwa kwevanhu (> 20 kHz).Ivo vanoshanda mukuchinjika oscillation, vachiisa maitiro emhando yemafundo uye antinodes pamwe nehurefu hwawo.Izwi rekuti "passive ultrasonic irrigation" (PUI) iprotocol yekudiridza umo pasina zviridzwa kana madziro zvinosangana ne endodontic mafaera kana zviridzwa.Munguva yePUI, simba re ultrasound rinotamiswa kubva kune faira rekuzunguza kuenda kumhinduro yekudiridza mumudziyo wemidzi.Iyo yekupedzisira inogona kukonzera kuyerera kwe sonic uye cavitation yemumiriri we flushing.11 Kubva pane data riri pamusoro, zvinoonekwa zvakakodzera kushandisa nanotechnology kuongorora yakavandudzwa kupinza nekuchenesa chiito cheChx.HCl.
Chlorhexidine hydrochloride Chx.HCl yakapihwa nemutsa neArab Drug Company for Pharmaceuticals (Cairo, Egypt).Labrafil M 1944 CS (oleoylpolyoxy-6-glyceride) yakapiwa nerupo neGattefosse (Saint Priest, France).Pakati pe20 (polyoxyethylene (20) sorbitan monolaurate), Pakati pe80 (polyoxyethylene (80) sorbitan monooleate), oleic acid, propylene glycol kubva kuGomhorya Company (Cairo, Egypt)).Kubviswa kwemazino asina-carious ane midzi midzi ye periodontal kana orthodontic kurapwa, Dhipatimendi reMaxillofacial Sayenzi, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.Yakachena tsika yeEnterococcus faecalis (strain ATCC 29212) yakakura muuropi moyo inobvisa (BHI) muto (RC CLEANER, IIchung Dental Ltd., Seoul, Korea).
Iyo solubility yeChx.HCl mumhando dzakasiyana-siyana (oleic acid, Labrafil M 1944CS, Pakati pe20, Pakati pe80, propylene glycol, uye mvura) yakadzidzwa.Kuwedzera kukuru kweChx.HCl (50 mg) inoiswa muchubhu ye centrifuge uye 5.0 g yepakati yepakati inowedzerwa.Musanganiswa wacho wakazununguswa muvhenganisi yevortex kwemaminitsi gumi nemashanu uye wozochengetwa mukamuri yekupisa.Mushure memaawa makumi maviri nemana, iyo insoluble drug pellet muchubhu yaive centrifuged pa3000 rpm kwemaminetsi mashanu kuti iwane yakajeka supernatant.Unganidza yakakwana mhinduro yemuenzaniso uye uinyudze ne n-butanol.Iwo masampuli akacheneswa akasefa kuburikidza neWhatman 102 sefa bepa uye ndokunatswa zvakafanira nen-butanol kuti aone kuwanda kwezvinodhaka mune yakazadzwa mhinduro.Samples akaongororwa neUV spectrophotometer pa260 nm ine n-butanol sekutonga.12.13
A pseudo-triple phase diagram yakavakwa kuti ione chiyero chaicho chechikamu chimwe nechimwe chinodiwa mukugadzirisa kuti uwane zvakakwana zvigadziro zvenanoemulsion yakanaka.14 Kugadzirwa kwakagadzirwa nemafuta (kureva oleic acid neLabrafil M1944CS), ma surfactants (kureva pakati pe20 nepakati pe80) uye imwe surfactant, kureva propylene glycol.Chekutanga, musanganiswa wakapatsanurwa we surfactants (pasina cosurfactants) nemafuta akagadzirwa mumhando dzakasiyana dzevhoriyamu (kubva pa1: 9 kusvika 9: 1).Kana musanganiswa uchinge waiswa mvura (kuwedzera mvura inodonha), nyatso tarisa musanganiswa kubva pakajeka kusvika kune makore semagumo.Aya mapoinzi ekupedzisira anozomakwa pane pseudo-triple phase diagram.Iyo yese nzira yakadzokororwa kune misanganiswa ye surfactant uye yechipiri surfactant (Smix) yakagadzirirwa mu2: 1 uye 3: 1 reshiyo uye yakasanganiswa nemafuta akasarudzwa15,16 imwe.
Nanoemulsion systems dzine Chx.HCl dzakagadzirirwa kushandiswa kweLabrafil M 1944 CS sechikamu chemafuta uye Pakati pe80 kana 20 surfactant uye propylene glycol sekuwedzera surfactant uye pakupedzisira mvura, Tafura 1. Mushonga wacho wakanyungudutswa muLabrafil M 1944 CS uye mvura yakasanganiswa ye surfactant yakawedzerwa zvishoma nezvishoma ye surfactant surfactant.Huwandu hweiyo surfactant uye co-surfactant yakawedzerwa, pamwe nechiyero chechikamu chemafuta chinogona kuwedzerwa, chinotemerwa uchishandisa pseudo-ternary phase diagram.An ultrasonic jenareta (Ultrasonic LC 60 H, Elma, Germany) yakashandiswa kuzadzisa yaidiwa saizi renji yekuparadzira magranules.Zvadaro zvienzanise.17
Kuongororwa kweDispersibility kwakaitwa pachishandiswa dissolution apparatus (Dr. Schleuniger Pharmaton, Model Diss 6000, Thun, Switzerland) umo 1 ml yegadziriro yega yega yakawedzerwa ku500 ml yemvura pa37±0.5°C.Kugwinya kunyoro kunovimbiswa nesimbi yakajairwa isina tsvina inonyungudutsa mapadhi anotenderera pa50 rpm.Iyo emulsion yakaguma yakatemwa nekuona uye yakarongerwa seyakajeka, inopenya ine bluish tinge, mukaka kana hazy.Sarudza fomura yakajeka yekuenderera mberi nekutsvakurudza.18.19
Kubviswa kweChx.HCl kubva kune yakagadziridzwa nanoemulsion zvinyorwa zvichibva pane pseudo-triple phase diagram inotungamirira kugadzirwa kwen-butanol uchishandisa ultrasonic teknolojia.Mushure mekunatswa kwakakodzera, zvinyorwa zvakaongororwa spectrophotometrically pawavelength ye260 nm yezviri muChx.HCl.makumi maviri
Kuti uedze nguva ye-self-emulsification, 1 ml yega yega yakagadzirwa yakawedzerwa kune beaker yakazadzwa ne 250 ml yemvura yakasvibiswa uye yakachengetwa pa 37 ± 1 ° C nekugara ichikurudzira pa 50 rpm.Iyo yekuzvimisa-emulsification nguva inotorwa senguva iyo iyo preconcentrate inoumba homogeneous musanganiswa mushure mekunatswa.makumi maviri nerimwe
Kuti uongorore saizi yedonhwe, deredza 50 mg yeyakagadziriswa dhizaini kusvika ku1000 ml nemvura muflask uye sanganisa zvinyoro nemaoko.Droplet saizi yekugovera yakatemwa uchishandisa Malvern Zetasizer 2000 chiridzwa (Malvern Instruments Ltd., Malvern, UK) pasi pe backscatter yekuona mamiriro e173º, tembiricha ye25ºC, uye refractive index ye1.330.makumi maviri nemaviri
In vitro dissolution zvidzidzo zvakaitwa uchishandisa USP Type II apparatus (paddle) (Dr. Schleuniger Pharmaton, Diss Model 6000) pa50 rpm.Mvura yakasvibiswa (500 ml) yakachengetedzwa pakupisa kwe 37 ± 0.5 ° C yakashandiswa semhepo yakasvibiswa, uye 5 ml yekugadzirwa kwakagadzirirwa yakawedzerwa kudonha kusvika kune yakasvibiswa.Zvino, panguva dzakasiyana-siyana, 5 ml yemhepo yekunyungudutsa yakatorwa uye huwandu hwemushonga wakaburitswa hwakatemwa spectrophotometrically pa254 nm.Iwo maedzo akaitwa akapetwa katatu.makumi maviri nenhatu
Zvadaro, kinetic parameters yeChx.HCl release in vitro kubva nanoemulsions yakagadzirirwa pahwaro hwayo yakayerwa.Zero, yekutanga uye yechipiri kinetics uye Higuchi diffusion mienzaniso yakaedzwa kuti isarudze kinetic sequence inonyatsokodzera kusunungurwa kweChx.HCl.
2 ml yega yega yakagadzirwa yakachengetwa pakupisa kwemaawa makumi mana nemasere kusati kwaonekwa kuparadzaniswa kwechikamu.1 ml samples yeChx.HCl imwe neimwe nanoemulsion formulation yakabva yaderedzwa kusvika ku10 ml uye 100 ml nemvura yakasvibiswa pa25 ° C. uye yakachengetwa kwemaawa makumi maviri nemana.Ipapo kuparadzaniswa kwechikamu kwakaonekwa.makumi maviri nerimwe
Zvadaro zvienzaniso zve 2 ml yeimwe neimwe yakagadzirwa zvakaendeswa zvakasiyana mumabhodhoro akajeka ane screw cap uye akachengetwa mufiriji pa 2 ° C kwemaawa makumi maviri nemana.Zvadaro zvakabviswa ndokuchengetwa pa25°C uye 40°C.Kamwe kutonhora-kunyorova kutenderera kwakaitwa.Iwo masampuli akabva acherechedzwa kwekuparadzaniswa kwechikamu uye kunaya kwezvinodhaka.makumi maviri nerimwe
Sample ye5 ml yega yega Chx.HCl nanoemulsion formulation yakaendeswa muchubhu yegirazi ndokuiswa mulabhoritari centrifuge (Shanghai Surgical Instrument Factory Microcentrifuge Model 800, Shanghai, People's Republic of China) uye centrifuged pa4000 rpm kwemaminitsi mashanu.Iwo masampuli akabva acherechedzwa kwekuparadzaniswa kwechikamu uye kunaya kwezvinodhaka.makumi maviri nerimwe
Zvese zviedzo zvakatenderwa neInstitutional Ethics Committee yeAin Shams University, Egypt.Mazino makumi mashanu asina-carious ane midzi yemunhu ane apex akaumbwa akasarudzwa.Mazino akabviswa akashandiswa mushure mekuwana mvumo yakanyorwa ine ruzivo yakasainwa nemurwere.Mazino anosanganisira maxillary uye mandibular incisors uye mandibular premolars.Mamiriro ekunze emidzi akabatwa ne curette uye mazino ose akaiswa pasi pekuita sterilization mu 0.5% NaOCl kwemaawa makumi maviri nemana uye akazochengetwa mu sterile saline kusvika kushandiswa.Korona yakabviswa nedivi rakachengeteka redhaimani disc uye kureba kwezino kwakagadziridzwa kusvika 16 mm kubva kumusoro kusvika coronal margin.24,25 Zvinoenderana nemhinduro yekusuka, mazino akakamurwa kuita mapoka anotevera:
(A) Boka (n = 24) sampuli dzakashambidzwa neChx.HCl nanoemulsion.Subgroup (I) (n = 12) yakagezwa samples ne 5 ml Chx.HCl nanoemulsion 0.75% concentration.Subgroup (II) (n=12) yakasuka sampuli ne 5 ml ye 1.6% Chx.HCl nanoemulsion.(B) Boka (n=24) remasamples richagezwa ne5 ml 2% Chx.HCl normal particle size.Boka rekudzora: (n = 2) yakashambidzwa ne5 ml saline pasina activation.
Akasarudzwa 44 asina-carious mazino emunhu akadzika midzi ane muromo wakaumbwa.Mazino anosanganisira maxillary uye mandibular incisors uye mandibular premolars.Mamiriro ekunze emidzi akabatwa ne curette uye mazino ose akaiswa pasi pekuita sterilization mu 0.5% NaOCl kwemaawa makumi maviri nemana uye akazochengetwa mu sterile saline kusvika kushandiswa.Makona akabviswa nedhaimani disc yekuchengetedza uye kureba kwezino kwakajairwa kusvika 16 mm kubva kumusoro kusvika coronal margin.24,25,29
Mechanical kugadzirira kweiyo huru apical faira saizi 50 uchishandisa yakajairwa nzira.Shandisa sterile saline sechinhu chinodiridza panguva yekuvhiyiwa.Pakupedzisira, midzi yemidzi yakasvibiswa ne 2 ml ye17% EDTA kweminiti 1 kubvisa smear layer.Midzi yose yepasi, kusanganisira apical foramen yemuenzaniso wega wega, yakafukidzwa nejasi rembambo yezvipikiri (cyanoacrylate glue) kudzivirira kubuda.Mazino anobva agadzikwa vertically mubhokisi retartar kuitira nyore kubata nekuzivikanwa.29-33 Samples dzakabva dzave autoclaved pa121ºC uye 15 psi kwemaminetsi makumi maviri.Mushure mekuita sterilization, ese masampula akatakurwa uye akagadziriswa pasi pemamiriro ekudzivirira pachishandiswa midziyo isina mbereko.Midzi yemidzi yakasvibiswa netsika yakachena yeEnterococcus faecalis (strain ATCC 29212) yakakura muuropi yemoyo inobvisa (BHI) muto kwemaawa makumi maviri nemana pa37 ° C.Uchishandisa sterile micropipette, jekiseni kumira kwakajeka kweE. faecalis inoculum mumigero yemidzi yakagadzirwa yemazino ose.Mabhuroko acho akabva aiswa mumabheki asina utachiona ndokuiswa pa37°C kwemaawa makumi maviri nemana.31, 34, 35
(A) Boka (n = 24) sampuli dzakashambidzwa neChx.HCl nanoemulsion.Samples of subgroup (I) (n = 12) yakashambidzwa ne 5 ml yeChx.HCl nanoemulsion 0.75% concentration.Subgroup (II) (n = 12) yakasuka sampuli ne 5 ml yeChx.HCl nanoemulsion 1.6% concentration.
Boka rekudzora: kutonga kwakanaka, (n = 4) midzi yakasvibiswa yakasvibiswa yakasvibiswa ne 5 ml saline uye yakachengetwa sekutonga kwakanaka.Negative Control: (n = 4) Mienzaniso yakanga isina kuiswa jekiseni nekumiswa, kureva kuti midzi yemidzi yakanga isina kusvibiswa neE. faecalis, uye yakachengetwa isina kuchena sekutonga kusina kunaka kusimbisa sterilization uye kuvimbika kwemaitiro.Shandisa 5 ml test yekugeza mhinduro mumuenzaniso wega wega.Muenzaniso wega wega wakabva waiswa pakugezwa kwekupedzisira ne 1 ml yesaline isina utachiona.
Saizi makumi matatu neshanu bepa retipi rinoshandiswa kuunganidza sampuli kubva mumidzi migero.Iyo bepa repi yakapinzwa muchubhu kusvika paurefu hwekushanda, yasiiwa kwemasekonzi gumi, yozoendeswa kumapureti eagar kuona huwandu hwemakoloni ekugadzira mayunitsi (CFU) pandiro.Mahwendefa acho akaiswa pa37ºC kwemaawa makumi maviri nemana uye oongororwa nemaziso kuona kukura kwebhakitiriya.Iyo ndiro inoonekera inoratidza sterilization yakakwana.Blurred plates anoonekwa sekuratidza kukura kwakanaka.Avhareji yenhamba yeCFU munzvimbo yekukura kwebhakitiriya pandiro yakatemwa uye nhamba yeCFU yakaverengerwa.Vapukunyuki vanoyerwa zvakanyanya nezviverengero zvinogoneka pamadrip plates.Mukuwedzera, mukombe wekudira wakashandiswa kuverenga maCFU akaderera, uye dilution kusvika 106 yakashandiswa kuverenga maCFU akakwirira.36.37
Gadzirira machubhu ane 15 ml yeakanyungudutswa agar yepakati pre-yakaomeswa mune autoclave pazuva rimwechete seyeyedzo.Enterococcus faecalis inyanzvi yeGram-positive anaerobic coccus inogona kurarama pakakwirira kwazvo pH, acidity uye tembiricha yakakwira.39 Bacterial samples (Enterococcus faecalis ATCC 29212) yakagadzirwa nekusanganisa masero kubva kumakoloni ane sterile saline.Iwo mabhakitiriya samples akabva adirwa nesaline kuti aenderane neMcFarland 0.5, yakaenzana ne108 CFU/mL.Muenzaniso wevhoriyamu yakawedzerwa yaive 10 µl.39 A turbidity standard (McFarland 0.5)40 yakagadzirwa nekudirwa 0.6 ml ye1% (10 g/l) barium chloride dihydrate solution mumumburu wakaitwa 100 ml uye uchizadza kusvika ku100 ml ne1% (10 g/l) sulfuric acid.Turbidity zviyero zvakaiswa mumachubhu akafanana nemasampula emuto uye zvakachengetwa mukamuri tembiricha kwemwedzi mitanhatu murima uye yakavharwa kudzivirira kubuda.Vhura chivharo chePetri dish isina chinhu uye udururire sampuli mukati mendiro.Kana iyo agar yakanyatsosimba, inverted ndiro uye incuite pa 37 ° C kwemaawa makumi maviri nemana.
Yese data yakaunganidzwa, yakarongwa uye yakaiswa pasi pekuongorora kwenhamba.Ongororo yenhamba yakaitwa pachishandiswa IBM® SPSS® Statistical Version 17 yeWindows (SPSS Inc., IBM Corporation, Armonk, NY, USA).
Kunyungudika kweChx.HCl muzvikamu zvakasiyana-siyana zvemafuta, surfactant solutions, co-surfactant solutions, uye mvura zvakadzidzwa.Chx.Hcl ine yakanyanya solubility muLabrafil M uye yakaderera solubility muoleic acid.Kunyanya kunyungudika kwezvinodhaka muchikamu chemafuta kunokosha kune nanoemulsions nokuti nanoemulsions inokwanisa kuchengetedza mushonga wakanyunguduka, zvinoreva kuti kunyunguduka kwezvinodhaka mumafuta kunoguma nemafuta mashoma mukuumbwa uye naizvozvo zvinodhaka zvishoma.kurodha Imwe chiyero chesurfactant uye co-surfactant inodiwa kuti emulsify madonhwe emafuta.
A pseudo-triple phase diagram yakagadzirwa kuti itsanangure nanoemulsion matunhu uye kugadzirisa kuwanda kwemafuta akasarudzwa, ma surfactants, uye mamwe ma surfactants (Labrafil M, Pakati pe80, Pakati pe20, uye propylene glycol, zvichiteerana).Chx.Hcl inoratidza kunyungudika kwakaderera muoleic acid, zvichiita kuti pave nemakore apo oleic acid inenge yaderedzwa nedonho rekutanga remvura.Naizvozvo, iyo oleic acid system yakabviswa muchidzidzo ichi.Mamwe magadzirirwo akagadzirwa uchishandisa 1: 9 musanganiswa wemafuta uye surfactant.huwandu hwepH uye ionic simba, saka ma surfactants aya akasarudzwa.
Ese magadzirirwo akagadzirwa aive akajeka kunze kweSystem F2, iyo yairatidzika kunge yakasviba uye saka yakabviswa kubva kune zvimwe zvidzidzo zvekuongorora.
Iyo yakakodzera nanoemulsion formulation inofanirwa kukwanisa kupararira zvachose uye nekukurumidza kana yakanyungudutswa nekunyorovesa kushushikana.Chx.HCl nanoemulsion formulations yakaratidza nguva shoma emulsification, kubva pa1.67 kusvika ku12.33 seconds.Pakati pe80 ine ipfupi emulsification nguva.Izvi zvinogona kutsanangurwa nepamusoro-soro solubilizing simba reTween 80. Nguva ye-self-emulsification inowedzera nekuwedzera surfactant concentration, iyo inogona kunge yakakonzerwa nekuwedzera kwe viscosity yegadziriro pasi pechiito che surfactant.
Ukuru hwemadonhwe emulsion inotarisa chiyero uye chiyero chekusunungurwa kwezvinodhaka.Diki emulsion droplet size inokonzeresa kupfupika emulsification nguva uye yakawanda nzvimbo yekutorwa kwezvinodhaka.Avhareji yemadonhwe saizi ezvinyorwa zvakasarudzwa zveChx.HCl nanoemulsion aive 711±0.44, 587±15.3, 10.97±0.11, 16.43±4.55, uye 12.18±2.48, uye PDI yaive 0 .76, 0, 0, 0.7 F. ., F3 uye 0.16 zvakateerana F4, F5 uye F6.Magadzirirwo ane Tween 80 sea surfactant airatidza madiki spherulites.Izvi zvinogona kunge zviri nekuda kwesimba rayo repamusoro emulsifying.Iyo yakaderera PDI kukosha inoratidza yakamanikana system saizi kugovera.Aya magadzirirwo ane chitarisiko chakachena nekuti madonhwe radii awo idiki pane yekuona wavelength yechiedza chinooneka (390-750 nm) apo kupararira kushoma kunoitika.41
Pamusoro pemuonde.2 inoratidza chikamu cheChx.HCl chakaburitswa kubva mugwaro rakagadzirwa.Kusunungurwa kwakakwana kwechirwere kubva kune zvakagadzirirwa zvakagadzirirwa zveChx.HCl nanoemulsion yakabva ku2 kusvika kumaminitsi manomwe.Yakaonekwa kuti chiyero chepamusoro chekusunungurwa kwezvinodhaka chakawanikwa munyaya yeChx.HCl F6 nanoemulsion formulation (2 min), iyo inogona kunge yakakonzerwa nekuvapo kweTween 80, iyo yakaratidza dhigirii yepamusoro ye emulsification, uye inoguma nanoemulsion.inopa nzvimbo yakakura yepamusoro yekusunungurwa kwezvinodhaka, zvichibvumira kuwedzera kusunungurwa kwezvinodhaka.Panguva imwecheteyo, iyo solubility properties yepropylene glycol inobvumira yakawanda yakawanda ye hydrophilic surfactants kuti iparadzwe mumafuta.40
Chx.HCl release in vitro yakawanikwa ichitevera kinetic order yakasiyana, uye hapana zvakajeka kinetic order inogona kuratidza kusunungurwa kwezvinodhaka kubva kune zvakasiyana-siyana zvakagadzirirwa nanoemulsion formulations.Kinetic kusunungurwa kwemishonga yeF4 ndiyo yekutanga kurongeka kinetics, zvinoreva kuti inosunungurwa maererano nehuwandu hwemishonga yakasara mukati mavo.42 Kinetic kuburitswa kwemamwe mishonga yaienderana neiyo Higuasha diffusion modhi, iyo yairatidza kuti huwandu hwemushonga hwakaburitswa hwaienderana neskweya mudzi weiyo yakazara mushonga uye kunyungudika kwezvinodhaka mu nanoemulsion.42
Maumbirwo akasarudzwa akaiswa mukusiyana kwe thermodynamic kugadzikana kuburikidza nekunetseka kuyedzwa uchishandisa kupisa-kutonhorera kutenderera, centrifugation uye kutonhora-kunyungudika kutenderera.Zvakaonekwa kuti maumbirwo eF3 neF4 airatidza kunaya kwemushonga mushure mekunyunguduka, ukuwo F1 yairatidza kukora (gelling).F5 uye F6 yakapfuura inoenderera centrifugation cycle, kupisa-kutonhora bvunzo uye nechando-thaw bvunzo.Nanoemulsions ndiyo thermodynamically stable systems inoumbwa pane mamwe mafuta, surfactant uye mvura pasina kupatsanurwa kwechikamu, emulsification kana kuputika.Iko kugadzikana kwekushisa kunosiyanisa nanoemulsions kubva emulsions, iyo inogadzikana kinetically uye inozopedzisira yakaparadzana muzvikamu.19 F3 yakaratidza saizi yakakura yechikamu (587 nm) pane mamwe magadzirirwo, ayo anogona kutsanangura kupatsanurwa kwechikamu uye kunaya kwezvinodhaka muyedzo yekugadzikana kwe thermodynamic.F4 ine Tween 80 uye hapana co-surfactant yakaratidza kunaya kwezvinodhaka, izvi zvinogona kuratidza pane kudiwa kwekushandisa propylene glycol uye Pakati pe80 kuvandudza kugadzikana kweanomulsion formulations.F1 ine Tween 20 isina yekuwedzera surfactant inoratidzwa thickening (gelling), iri kuwedzera kwegel viscosity kana simba nekuda kwekudonhedza aggregation.
Migumisiro yekugadzikana inoratidza kukosha kwekuvapo kweimwe propylene glycol surfactant kuti iwedzere kupararira kwechidimbu uye kudzivirira kunaya kwezvinodhaka.43 F6 ndiyo yaive yakanakisa kuumbwa nekuda kweiyo diki diki saizi (12.18 nm), pfupi emulsification nguva (1.67 masekonzi) uye nekukurumidza kupera kwekupera mushure memaminitsi maviri.Yakawanikwa iine thermodynamically / stable stable system uye saka yakasarudzwa kuti iwedzere kudzidza.
Kukundikana mushure mekurapa kwemidzi yemidzi iri kuramba ichiwedzera, zvinoreva kuti varwere vari pangozi yekuwedzera zvirwere zvakaoma.44,45 Biofilm inofanira kubviswa panguva yekuuraya utachiona uye kuzadza midzi yemidzi.46,47 Pamusana pekuoma kwemidzi yemidzi, zvinova zvakaoma kubvisa zvachose mabhakitiriya midzi migero uchishandisa zvishandiso uye kudiridza.48 Kushanda kwemudzi wemvura yekusuka mhinduro kunoenderana nekupinda kweinodiridza muDT uye nguva yekusangana nebhakitiriya.49 Naizvozvo, nzira itsva dzekunyatso svira midzi dzakaedzwa nekuedzwa.Maresheni akajairwa haabvisi zvachose E. faecalis nekuda kwekupinda kushoma kweDT.50.
Avhareji yesimba rekuchenesa re nanoemulsion rinse yaive 2001.47 µm2, uye avhareji saizi yeaid yekusuka yaive 2609.56 µm.Avhareji mutsauko pakati pe nanoemulsion wash uye yakajairika particle size wash yaive 608.09 µm2. Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pe nanoemulsion irrigants uye yakajairika particle size irrigants ne (P-value 0.00052). Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pe nanoemulsion irrigants uye yakajairika particle size irrigants ne (P-value 0.00052). Между ирригационными растворами наноэмульсии и ирригационными растворами с нормальным размером частиц наблюдалась статистистически зназ,машение чение P 0,00052). Paive nenhamba yakakosha (P <0.001) musiyano (P kukosha 0.00052) pakati pe nanoemulsion irrigants uye yakajairika particle irrigants.纳米乳液冲洗剂和正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P<0.001） 0.纳米乳液冲洗剂和正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P<0.001） 0. Между ополаскивателем с наноэмульсией и ополаскивателем с нормальным размером частиц была статистически очень значимая разница,500,500 (P2000,500,10). Paive nemusiyano wakanyanya kukosha (P <0.0001) pakati penanoemulsion rinse uye yakajairika particle size rinse (P kukosha 0.00052).Iyo nanoemulsion yakaratidza mutsauko wakakosha zvakanyanya kana uchienzaniswa neyakajairwa particle size material, ichiratidza yakaderera inorevesa yasara marara pamusoro penzvimbo, kureva nanoemulsion zvinhu zvaive nehunyanzvi hwekuchenesa, sezvakaratidzwa mumufananidzo 3.
Mufananidzo 3. Kuenzanisa kwekuchenesa kushanda kwemishonga yekuchenesa: (A) neNano CHX laser yakagadziriswa, (B) ine CHX laser inoshandiswa, (C) nePUI Nano CHX, (D) pasina Nano CHX activation, (E) pasina CHX kushandiswa, uye (F) ) CHX PUI activation.
Avhareji yenzvimbo yakasara yeChx.HCl 1.6% zvimedu yaive 2320.36 µm2, uye avhareji yenzvimbo yeChx.HCl 2% yaive 2949.85 µm2. Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pehuwandu hwepamusoro hwe nanoemulsion irrigants uye yakajairika particle size irrigants (P-value 0.00000). Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pehuwandu hwepamusoro hwe nanoemulsion irrigants uye yakajairika particle size irrigants (P-value 0.00000). Наблюдалась статистически высокозначимая (P<0,001) разница между более высокой концентрацией наноэмульсионных ирриганциранська ворами с нормальным размером частиц (значение P 0,00000). Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pehuwandu hwepamusoro hwe nanoemulsion irrigants uye yakajairika particle size irrigants (P kukosha 0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的（0P0. 0000.较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学显着的差异（001P0. Наблюдалась статистически значимая разница (P<0,001) между более высокими концентрациями ополаскивателя разница (P<0,001) между более высокими ополаскивателя разница мером частиц (значение P 0,00000). Paiva nemusiyano wakanyanya kukosha (P <0.001) pakati pehuwandu hwepamusoro hwe nanoemulsion rinse uye yakajairika particle size rinse (P kukosha 0.00000).Kunyangwe kuwanda kweiyo nanoemulsion irrigant yaive yakaderera pane iyo yakajairwa particle size irrigant, iyi yakaderera yainyanya kushanda pakubvisa marara uye zvakanyanya kushanda mukuchenesa midzi migero.
PUI yaive nemusiyano wakanyanya kukosha (p <0.001) kana ichienzaniswa nedzimwe nzira dzekuita. PUI yaive nemusiyano wakanyanya kukosha (p <0.001) kana ichienzaniswa nedzimwe nzira dzekuita. PUI имел статистически высокозначимую разницу (p<0,001) по сравнению с другими методами активации. PUI yaive nemusiyano wakanyanya kukosha (p <0.001) zvichienzaniswa nedzimwe nzira dzekuita.与其他激活方法相比，PUI 具有统计学上非常显着的差异(p<0.001).与其他激活方法相比，PUI 具有统计学上非常显着的差异(p<0.001). По сравнению с другими методами активации PUI имел статистически значимую разницу (p<0,001). Kuenzaniswa nedzimwe nzira dzekuita, PUI yaive nemusiyano wakanyanya kukosha (p <0.001).Nekushandiswa kweISP, avhareji nzvimbo yenzvimbo yakasara yemarara yaive 1695.31 µm2. Musiyano unoreva pakati pePUI neLaser waive 987.89929 uchiratidza zvakanyanya kukosha (P <0.001) musiyano ne (p-value 0.00000). Musiyano unoreva pakati pePUI neLaser waive 987.89929 uchiratidza zvakanyanya kukosha (P <0.001) musiyano ne (p-value 0.00000). Средняя разница между PUI uye Laser составила 987,89929, демонстрируя высокостатистически значимую (P<0,001) разницу с (p-значение 000). Musiyano unoreva pakati pePUI neLaser waive 987.89929, uchiratidza mutsauko wakakosha (P <0.001) kubva (p-kukosha 0.00000). PUI 和Laser 之间的平均差异為987.89929，显示出高度统计学显着性(P<0.001) 差异(p 值0.0000).PUI uye Laser Средняя разница между PUI uye Laser составила 987,89929, что свидетельствует о высокой статистической значимости (P<0,001) разниц00 (P<0,001) разниц00 (P<0,001) разни000 Musiyano unoreva pakati pePUI neLaser waive 987.89929, zvichiratidza kukosha kwehuwandu hwepamusoro (P <0.001) musiyano (p-value 0.00000). Musiyano unoreva pakati pePUI uye hapana activation yaive 712.40643 ichiratidza mutsauko wakanyanya kukosha (P <0.001) musiyano ne p-value ye0.00098).Kushandiswa kweLaser activation kana kusaitwa kwakange kusiri zvakanyanya statistically (P>0.05) yakasiyana neP-value ye0211. Musiyano unoreva pakati pePUI uye hapana activation yaive 712.40643 ichiratidza yakanyanya kukosha (P <0.001) mutsauko ne p-value ye0.00098).P-kukosha kwe0.451211. Средняя разница между PUI и отсутствием активации составила 712,40643, демонстрируя высокостатистически значимую (P<0,0001, p. Musiyano unoreva pakati pePUI uye hapana activation yaive 712.40643, ichiratidza mutsauko wakakosha (P <0.001) ne p-value ye0.00098).P-kukosha 0.451211. PUI 和未激活之间的平均差异為712.40643，显示高度统计学显着性差异(P<0.001)，p 0006430).PUI Средняя разница между PUI и инактивацией составила 712,40643, что свидетельствует о высокой статистической значимости раз,0000,p90,800 (P900000,p90,800, p. Musiyano unoreva pakati pePUI uye kusashanda waive 712.40643, zvichiratidza kukosha kwehuwandu hwemusiyano (P <0.001, p-kukosha 0.00098).使用激光激活或不激活在统计学上没有显着差异(P>0.05) P 值為0.451211.使用激光激活或不激活在统计学上没有显着差异(P>0.05) P 值為0.451211. Статистически значимой разницы (P>0,05) с лазерной активацией или без нее не было со значением P 0,451211. Pakanga pasina mutsauko wakakosha (P> 0.05) ine kana isina laser activation ine P kukosha kwe0.451211.Avhareji yenzvimbo yezvimedu zvakasara pane laser activation yaive 2683.21 µm2.Avhareji yenzvimbo yezvimedu zvakasara pasina activation yaive 2407.72 µm2.Kuenzaniswa nelaser activation kana kusaita activation, PUI yaive nenhamba diki avhareji yepakati chip nzvimbo, kureva simba rekuchenesa rakanakisa.
Avhareji yesimba rekuchenesa re nanoemulsion rinse yaive 2001.47 µm2, uye avhareji saizi yeaid yekusuka yaive 2609.56 µm.Avhareji mutsauko pakati pe nanoemulsion wash uye yakajairika particle size wash yaive 608.09 µm2. Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pe nanoemulsion irrigants uye yakajairika particle size irrigants ne (P-value 0.00052). Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pe nanoemulsion irrigants uye yakajairika particle size irrigants ne (P-value 0.00052). Между ирригационными растворами наноэмульсии и ирригационными растворами с нормальным размером частиц была статистически высояние земя 0,00052). Paive nenhamba yakakosha (P <0.001) musiyano (P kukosha 0.00052) pakati pe nanoemulsion irrigants uye yakajairika particle irrigants.纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0（001（P<0.000P<0.000). P <0.001 (P值0.00052). Между ополаскивателем с наноэмульсией и ополаскивателем с нормальным размером частиц была статистически очень значимая разница,500,500 (P2000,500,10). Paive nemusiyano wakanyanya kukosha (P <0.0001) pakati penanoemulsion rinse uye yakajairika particle size rinse (P kukosha 0.00052).Kuenzaniswa neyakajairwa particle size material, iyo nanoemulsion ine statistically yakakosha mutsauko, ichiratidza yakaderera inorevesa yasara marara pamusoro penzvimbo, kureva Nanoemulsion zvinhu zvine zvirinani kuchenesa kugona sezvakaratidzwa mumufananidzo 3.
Avhareji yenzvimbo yakasara yeChx.HCl 1.6% zvimedu yaive 2320.36 µm2, uye avhareji yenzvimbo yeChx.HCl 2% yaive 2949.85 µm2. Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pehuwandu hwepamusoro hwe nanoemulsion irrigants uye yakajairika particle size irrigants (P-value 0.00000). Paiva nenhamba yakakosha zvikuru (P <0.001) musiyano pakati pehuwandu hwepamusoro hwe nanoemulsion irrigants uye yakajairika particle size irrigants (P-value 0.00000). P<0,001 с нормальным размером частиц (значение P 0,00000). Paiva nenhamba yakakosha (P <0.001) musiyano pakati pehuwandu hwepamusoro hwe nanoemulsion irrigants uye yakajairika particle size irrigants (P kukosha 0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的Ｎ显着的00P0. 000.较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的Ｎ显着的Ｎ显着的0显着的0显着. Наблюдалась статистически высокозначимая разница (P <0,001) между более высокими концентрациями ополаскивателя разница (P <0,001) мером частиц (значение P 0,00000). Paiva nemusiyano wakakosha wenhamba (P <0.001) pakati pehuwandu hwepamusoro hwe nanoemulsion rinse uye yakajairika particle size rinse (P kukosha 0.00000).Kunyangwe kuwanda kweiyo nanoemulsion irrigant yaive yakaderera pane iyo yakajairwa particle size irrigant, iyi yakaderera yainyanya kushanda pakubvisa marara uye zvakanyanya kushanda mukuchenesa midzi migero.
PUI yakanga ine nhamba yakakura yakakosha musiyano (p <0.001) kana ichienzaniswa nedzimwe nzira dzekuita. PUI yakanga ine nhamba yakakura yakakosha musiyano (p <0.001) kana ichienzaniswa nedzimwe nzira dzekuita. PUI имел статистически высокую значимую разницу (p<0,001) по сравнению с другими методами активации. PUI yaive nemusiyano wakakosha (p <0.001) uchienzaniswa nedzimwe nzira dzekuita.与其他激活方法相比，PUI 具有统计学上的显着差异(p<0.001). Kuenzaniswa nedzimwe nzira dzekuita, PUI ine mutsauko wakakosha (p <0.001). PUI статистически значимо отличался (p<0,001) по сравнению с другими методами активации. PUI yakanga yakasiyana zvakanyanya (p <0.001) ichienzaniswa nedzimwe nzira dzokushandisa.Munguva yekushandiswa kwePUI, avhareji nzvimbo yezvakasara pamusoro pemarara yaive 1695.31 μm2. Mutsauko unoreva pakati pePUI neLaser waiva 987.89929 uchiratidza zvakanyanya statistically yakakosha (P <0.001) musiyano ne (p-value 0.00000) .The mean difference between PUI and no activation was 712.40643 ichiratidza zvakanyanya statistically yakakosha (P <0.001) musiyano we 0.0 0.0 actuvation kana 0.0-valuation activation 0.0. yakanga isina nhamba yakanyanya (P> 0.05) yakasiyana ne (P-kukosha 0.451211). Musiyano unoreva pakati pePUI neLaser waive 987.89929 uchiratidza zvakanyanya kukosha (P <0.001) musiyano ne (p-value 0.00000).Musiyano unoreva pakati pePUI uye hapana activation yaive 712.40643 inoratidza zvakanyanya statistically yakakosha (P <0.001) musiyano ne (p -value 0.00098).Iko kushandiswa kweLaser activation kana kusaita activation kwakanga kusina zvakanyanya statistically (P> 0.05) yakasiyana ne (P-value 0.451211). Средняя разница между PUI и лазером составила 987,89929, демонстрируя высокостатистически значимую (P<0,001) разницу с (p00,001) разницу с (p00,00). Musiyano unoreva pakati pePUI nelaser waive 987.89929, zvichiratidza mutsauko wakakosha (P <0.001) ne (p-value 0.00000). . - kukosha 0.00098).Kushandiswa kwelaser activation kana kusashanda kwaive nemusiyano wakakosha (P> 0.05) ne (P-kukosha 0.451211). PUI 和激光之间的平均差异為987.89929，与(p 值0.00000) 差异具有高度统计学意义(P<0.001) Musiyano wepakati pakati pePUI nelaser i987.89929, uye mutsauko (p 值0.00000) une kukosha kwehuwandu hwepamusoro (P <0.001). Средняя разница между PUI uye лазером составила 987,89929, что было высоко статистически значимым (P<0,001) с (значение p 0,000). Musiyano unoreva pakati pePUI nelaser waive 987.89929, iyo yaive yakanyanya kukosha (P <0.001) ine (p kukosha 0.00000). PUI 与未激活之间的平均差异為712.40643，与(p) 差异具有高度统计学意义(P<0.001.001) -0 090. Musiyano wepakati pakati pePUI uye isingashande ndeye 712.40643, uye mutsauko (p) une huwandu hwepamusoro kukosha (P <0.001) - kukosha 0.00098. Средняя разница между PUI и нактивацией составила 712,40643, что было высоко статистически значимым с разницей (p) (P<0,0001 —). Musiyano unoreva pakati pePUI uye kusashanda waive 712.40643, iyo yaive yakanyanya kukosha nemusiyano (p) (P <0.001 - kukosha 0.00098).使用激光激活或不激活没有显着统计学差异(P>0.05) 与(P 值0.451211). Pakanga pasina musiyano wakakosha wenhamba pakati pelaser activation uye isiri-activation (P> 0.05) uye (P 值0.451211). Не было статистически значимой разницы (P>0,05) по сравнению с (значение P 0,451211) с лазерной активацией или без нее. Pakanga pasina mutsauko wakakosha (P> 0.05) uchienzaniswa ne (P kukosha 0.451211) ine kana isina laser activation.Avhareji yenzvimbo yezvimedu zvakasara panguva yekushandiswa kwelaser yaive 2683.21 μm2.Avhareji yenzvimbo yezvimedu zvakasara pasina activation yaive 2407.72 μm2.Kuenzaniswa nelaser activation kana kusaita activation, PUI ine nhamba idiki avhareji yenzvimbo yechip, kureva kugona kuchenesa kurinani.
Izvo zvinoreva mhedzisiro ye nanoemulsion rinse pakubviswa kwemarara yaive yakakwira zvakanyanya kupfuura iyo yeyakajairwa particle size rinse.Chx.HCl 1.6%, PUI 1938.77 µm2, 2510.96 µm2 ine laser.Pasina activation, avhareji kukosha ndeye 2511.34 µm2.Apo 2% Chx.HCl yakashandiswa uye yakashandiswa nelaser, zvigumisiro zvaive zvakanyanya kuipa uye huwandu hwemarara hwakanga hwakanyanya.Migumisiro yakafanana yakawanikwa apo 0.75% Chx.HCl yakanga isina kushandiswa.Zviripachena, mhedzisiro yakanakisa yakawanikwa uchishandisa yakakwira kuwanda kwekusuka rubatsiro mu nanoemulsion.PUI yainyanya kushanda mukudiridza uye kuyerera kwemarara, sezvakaratidzwa mumufananidzo 3A-F)).
Sezvinoratidzwa muTebhurari 2, Chx.HCl nanoemulsion yakaita zviri nani kudarika mazamu akajairwa maererano nehuwandu hwehutachiona hwehutachiona uye hwakanga huine kuwirirana kwakanaka nekugadzirwa kwekupinda uye kuchenesa maitiro maererano nematanho anotevera: saizi, flushing agent concentration uye activation nzira.
Utachiona hunogona kuparadzwa zvachose nekushandisa huwandu hwepamusoro hwekusuka rubatsiro.Kunyangwe nePUI activation, 0.75% Chx.HCl yakanga ine yakaipisisa antibacterial effect.Laser activation ine mhedzisiro yakaipa pane nano-emulsion rinses.Sezvinogona kuonekwa kubva mumigumisiro yose yapfuura, kushandiswa kwe laser kunoderedza kushanda kweChx.HCl 0.75% nanoemulsion, apo CFU ye nanoChx.HCl 0.75% ndeye 195, iyo inonyanya kukosha, ichiratidza kuti reagents pamusangano uyu anofananidzwa ne laser activation.Diode lasers ndeye photothermal, saka chiedza kana kupisa kunogona kukonzera nanoemulsion kurasikirwa nehutachiona hwehutachiona.Mhedzisiro yepamusoro-soro ndiyo kuparadzwa kwakakwana kwebhakitiriya.Nano Chx.HCl 1.6% yakaratidza kukura kwebhakitiriya kwakashata muhupo hwe laser activation, zvinoreva kuti laser haina kukanganisa antibacterial kukwanisa nano Chx.HCl 1.6%.Zvinogona kugumiswa kuti nanoemulsion zvinhu zvine huwandu hwepamusoro hune nani antibacterial effect.
Mune basa iri, Chx.HCl nanoemulsions dzakagadzirirwa kushandiswa kwemafuta maviri akasiyana, maviri surfactants uye co-surfactant, iyo optimal formulation (F6) ine diki diki diki, pfupi emulsification nguva uye yakakwirira dissolution rate) yakasarudzwa.Mukuwedzera, (F6) yakaedzwa kugadzikana kwe thermodynamic / physical.MuChx.HCl nanoemulsion pamusangano we 1.6%, Chx.HCl nanoemulsion yakaratidza zvakanakisisa kupera mumakumbo emazino akaenzaniswa neChx.HCl yechinyakare semvura inosuka, uye PUI senzira yekugadzirisa yakanga ine simba rekuchenesa.Mukuwedzera, zvidzidzo zveantibacterial zveChx.HCl nanoemulsion zvakaratidza kubviswa kwakakwana kwebhakitiriya.Mibairo yakasimbisa izvi.Iyo Chx.HCl nanoemulsion inogona kutorwa semvura inovimbisa yekugeza.
Tinotenda zvikuru vashandi verabhoritari yetsvakurudzo yeMisr University of Science and Technology nerutsigiro rwavo rukuru.