Shanduko nyowani inline static musanganiswa yakagadziridzwa yakanyatso kugadzirwa kuti isangane neinoomesera zvinodiwa zveyakakwira performance liquid chromatography (HPLC) uye Ultra yakakwira performance liquid chromatography (HPLC uye UHPLC) masisitimu.Musanganiswa usina kunaka wezvikamu zviviri kana kupfuura zvefoni zvinogona kukonzera chiyero chepamusoro-kune-ruzha, izvo zvinoderedza kunzwa.Homogeneous static kusanganiswa kwezvinwiwa zviviri kana kupfuura zvine hushoma hwemukati vhoriyamu uye zvimiro zvemuviri zveiyo static musanganiswa inomiririra mwero wepamusoro weiyo yakanaka static musanganisi.Iyo nyowani static musanganiswa inozadzisa izvi nekushandisa nyowani 3D yekudhinda tekinoroji kugadzira yakasarudzika 3D chimiro chinopa yakagadziridzwa hydrodynamic static kusanganisa pamwe nepamusoro muzana kuderedzwa kwebase sine wave payuniti yemukati vhoriyamu yemusanganiswa.Kushandisa 1/3 yevhoriyamu yemukati yeyakajairwa musanganiswa inoderedza iyo basic sine wave ne98%.Iyo musanganiswa ine yakabatana 3D kuyerera nzira ine dzakasiyana-siyana-chikamu nzvimbo uye nzira kureba sezvo mvura inopfuura yakaoma 3D geometries.Kusanganisa pamwe neakawanda tortuous kuyerera nzira, dzakasanganiswa nemhirizhonga yemunharaunda uye eddies, zvinoguma mukusanganisa kune micro, meso uye macro zviyero.Uyu wakasarudzika musanganiswa wakagadzirwa uchishandisa computational fluid dynamics (CFD) simulations.Iyo data yekuyedza yakaratidzwa inoratidza kuti kusanganisa kwakanakisa kunowanikwa nehushoma hwemukati vhoriyamu.
Kweanopfuura makore makumi matatu, liquid chromatography yakashandiswa mumaindasitiri mazhinji, kusanganisira mishonga, mishonga yekuuraya zvipembenene, kuchengetedza kwezvakatipoteredza, forensics, uye kuongororwa kwemakemikari.Iko kugona kuyera kune zvikamu pamiriyoni kana zvishoma kwakakosha mukusimudzira tekinoroji mune chero indasitiri.Kusashanda zvakanaka kwemusanganiswa kunotungamira kune yakashata chiratidzo-kune-ruzha reshiyo, izvo zvinogumbura kunharaunda yechromatography maererano nemiganhu yekuona uye kunzwa.Kana uchisanganisa maviri HPLC zvinonyungudutsa, dzimwe nguva zvinodikanwa kumanikidza kusanganisa nekunze nzira kuti homogenize maviri zvinonyungudutsa nekuti zvimwe zvinonyungudutsa hazvisanganise zvakanaka.Kana zvinonyungudutsa zvisina kunyatsosanganiswa, kushatiswa kweHPLC chromatogram inogona kuitika, ichizviratidza seyakanyanya ruzha rwekutanga uye / kana yakashata peak chimiro.Nekusagadzikana kusanganisa, ruzha rwekutanga ruchaonekwa seine wave (kusimuka nekudonha) kweiyo detector chiratidzo nekufamba kwenguva.Panguva imwecheteyo, kusanganisa kwakashata kunogona kukonzera kuwedzera uye asymmetric peaks, kuderedza mashandiro ekuongorora, chimiro chepamusoro, uye peak resolution.Iyo indasitiri yakaona kuti mu-line uye tee static musanganiswa inzira yekuvandudza iyi miganho uye kubvumira vashandisi kuwana yakaderera yekuona miganho (sensitivities).Iyo yakanakira static musanganiswa inosanganisa mabhenefiti epamusoro musanganiswa kunyatsoshanda, yakaderera vhoriyamu yakafa uye yakaderera kudonha kudonha ine hushoma vhoriyamu uye yakanyanya system throughput.Mukuwedzera, sezvo kuongorora kunowedzera kuoma, vaongorori vanofanira kugara vachishandisa mamwe mapolar uye akaoma-kusanganisa-solvents.Izvi zvinoreva kuti kusanganisa kurinani kunofanirwa kuongororwa mune ramangwana, zvichiwedzera kuwedzera kudiwa kwepamusoro musanganiswa dhizaini uye kuita.
Mott ichangobva kugadzira huwandu hutsva hwepa patent PerfectPeak TM inline static mixers ine mavhoriyamu matatu emukati: 30 µl, 60 µl uye 90 µl.Aya saizi anovhara huwandu hwemavhoriyamu uye kusanganisa maitiro anodiwa kune mazhinji HPLC bvunzo uko kwakagadziridzwa kusanganisa uye kupararira kwakaderera kunodiwa.Ese mamodheru matatu ari 0.5 ″ mudhayamita uye anounza indasitiri-inotungamira kuita mune compact dhizaini.Iwo akagadzirwa ne 316L simbi isina tsvina, inopfuudzwa kune inertness, asi titanium uye kumwe kusingadzivirirwe uye makemikari inert simbi alloys iripo zvakare.Aya ma mixers ane yakanyanya kushanda kumanikidza kusvika ku20,000 psi.Pamusoro pemuonde.1a mufananidzo we60 µl Mott static musanganisi wakagadzirirwa kupa yakanyanya kusanganisa kunyatsoshanda uchishandisa vhoriyamu diki yemukati pane yakajairwa masanganisi emhando iyi.Iyi nyowani static musanganiswa dhizaini inoshandisa nyowani yekuwedzera yekugadzira tekinoroji kugadzira yakasarudzika 3D chimiro chinoshandisa kushoma kwemukati kuyerera kupfuura chero musanganiswa unoshandiswa muindasitiri yechromatography kuwana static musanganiswa.Misanganisi yakadai inoumbwa neakabatana-mativi matatu-eyekuyerera chiteshi ane akasiyana-chikamu-chikamu nzvimbo uye akasiyana nzira kureba sezvo mvura inoyambuka yakaoma geometric zvipingamupinyi mukati.Pamusoro pemuonde.Mufananidzo 1b unoratidza dhizaini remusanganiswa mutsva, iro rinoshandisa indasitiri yakajairwa 10-32 yakarukwa HPLC compression fittings yekupinza nekubuda, uye ine shaded yebhuruu miganhu ye patented yemukati yekusanganisa chiteshi.Nzvimbo dzakasiyana-siyana dzemuchinjiro wenzira dzekuyerera kwemukati uye shanduko yekuyerera mukati memukati wekuyerera kwevhoriyamu inogadzira matunhu emhirizhonga uye laminar kuyerera, zvichikonzera kusanganisa kune micro, meso uye macro zviyero.Dhizaini yemuvhenganisi uyu yakasarudzika yakashandisa computational fluid dynamics (CFD) simulations kuongorora mafambiro uye kunatsa dhizaini isati yaitwa prototyping ye-mumba yekuongorora bvunzo uye mutengi munda wekuongorora.Kuwedzera kugadzira inzira yekudhinda 3D geometric zvikamu zvakananga kubva kuCAD kudhirowa pasina kudiwa kwechinyakare machining (milling muchina, lathes, nezvimwewo).Aya matsva emusanganiswa anogadzirwa akagadzirwa kuti agadzirwe uchishandisa maitiro aya, uko musanganiswa muviri unogadzirwa kubva kuCAD kudhirowa uye zvikamu zvinogadzirwa (zvakadhindwa) dhizaini nedhiza uchishandisa yekuwedzera kugadzira.Pano, hupfu hwesimbi hunenge hupfu hwema microns makumi maviri hunoiswa, uye laser inodzorwa nekombuta inonyungudika nekusanganisa hupfu kuita hwakasimba.Isa imwe layer pamusoro peiyi layer uye isa laser sintering.Dzokorora nzira iyi kusvikira chikamu chapera zvachose.Iyo hupfu inobviswa kubva kune isiri-laser yakasungirirwa chikamu, ichisiya 3D yakadhindwa chikamu chinofanana neyekutanga CAD kudhirowa.Chigadzirwa chekupedzisira chakangofanana neiyo microfluidic process, nemusiyano mukuru uri wekuti iyo microfluidic components inowanzova maviri-dimensional (flat), nepo uchishandisa kuwedzera kwekugadzira, yakaoma kuyerera mapatani anogona kugadzirwa mumatatu-dimensional geometry.Aya mapombi parizvino ari kuwanikwa se 3D akadhindwa zvikamu mu316L Stainless simbi uye titanium.Mazhinji masimbi alloys, mapolymer uye mamwe maceramics anogona kushandiswa kugadzira zvikamu uchishandisa nzira iyi uye anozotariswa mune ramangwana dhizaini / zvigadzirwa.
Mupunga.1. Mufananidzo (a) uye dhayagiramu (b) ye90 μl Mott static mixer inoratidza muchinjiko-chikamu chemusanganisi wemvura inoyerera nzira ine mumvuri webhuruu.
Mhanyai computational fluid dynamics (CFD) simulations ye static mixer performance panguva yedhizaini dhizaini kuti ibatsire kugadzira madhizaini anoshanda uye kuderedza nguva-inodya uye inodhura kuedza-uye-kukanganisa kuyedza.CFD simulation ye static mixers uye yakajairika piping (hapana-musanganiswa simulation) uchishandisa iyo COMSOL Multiphysics software package.Kutevedzera uchishandisa kudzvanywa-inotyairwa laminar fluid mechanics kuti unzwisise kuyerera kwemvura uye kudzvanywa mukati mechikamu.Iyi fluid dynamics, yakasanganiswa nekutakurwa kwemakemikari yemobile phase compounds, inobatsira kunzwisisa kusanganiswa kwezvinwiwa zviviri zvakasiyana.Iyo modhi inodzidzwa sebasa renguva, yakaenzana negumi masekonzi, kuti zvive nyore kuverenga paunenge uchitsvaga mhinduro dzakafanana.Theoretical data yakawanikwa mune imwe nguva-yakabatana chidzidzo uchishandisa point probe projection tool, apo imwe pfungwa iri pakati pekubuda yakasarudzwa kuti iunganidze data.Iyo CFD modhi nemiedzo yekuyedza yakashandisa zvinonyungudutsa zviviri zvakasiyana kuburikidza neyakaenzanirana sampling vharafu uye yekupomba system, zvichikonzera kutsiva plug yeimwe solvent mumutsetse wesampling.Aya masolvents anobva asanganiswa mune static mixer.Zvifananidzo 2 uye 3 inoratidza kuyerera kwekuenzanisa kuburikidza nepombi yakajairwa (isina musanganisi) uye kuburikidza neMott static musanganiswa, zvichiteerana.Kufananidzira kwaiitwa pachubhu yakatwasuka 5 cm kureba uye 0.25 mm ID kuratidza pfungwa yekuchinjanisa mapulagi emvura uye yakachena acetonitrile muchubhu mukushayikwa kweiyo static mixer, sezvakaratidzwa muMufananidzo 2. Kufananidza kwakashandisa zviyero zvakakwana zvechubhu uye musanganisi uye kuyerera kwe 0 .3 ml / min.
Mupunga.2. Simulation yeCFD inoyerera muchubhu ye 5 cm ine dhayamita yemukati ye 0.25 mm kumiririra zvinoitika muchubhu yeHPLC, kureva kana pasina musanganisi.Tsvuku yakazara inomiririra chikamu chemvura.Bhuruu rinomirira kushaikwa kwemvura, kureva pure acetonitrile.Diffusion matunhu anogona kuonekwa pakati pealternating plugs ezvinwiwa zviviri zvakasiyana.
Mupunga.3. Static mixer ine volume ye30 ml, yakafananidzwa muCOMSOL CFD software package.Ngano inomiririra chikamu chikuru chemvura mumusanganisi.Mvura yakachena inoratidzwa mutsvuku uye yakachena acetonitrile mubhuruu.Shanduko muchikamu chikuru chemvura inofananidzwa inomiririrwa neshanduko yemuvara wekusanganiswa kwezvinwiwa zviviri.
Pamusoro pemuonde.4 inoratidza chidzidzo chekusimbisa cheiyo yekubatanidza modhi pakati pekusanganisa kugona uye kusanganisa vhoriyamu.Sezvo vhoriyamu yekusanganisa inowedzera, kusanganisa kunoshanda kuchawedzera.Kuruzivo rwevanyori, mamwe masimba emuviri akaomarara anoshanda mukati memusanganisi haagone kuverengerwa mune iyi CFD modhi, zvichikonzera kusanganisa kwakanyanya mukuyedza bvunzo.Iko kuedza kusanganisa kunyatsoshanda kwakayerwa sekuderedzwa kwezana muzana mubhesi sinusoid.Mukuwedzera, kuwedzera kwekumanikidzwa kwemashure kunowanzokonzera mazinga akakwirira ekusanganiswa, ayo asina kutorwa mukuenzanisa.
Aya anotevera HPLC mamiriro uye bvunzo yekumisikidza yakashandiswa kuyera mbishi sine mafungu kuenzanisa kuita kwakasiyana kweakasiyana static musanganiswa.Dhiagiramu iri muFigure 5 inoratidza yakajairwa HPLC/UHPLC system marongero.Iyo static musanganiswa yakaedzwa nekuisa musanganisi zvakananga mushure mepombi uye pamberi pejekitori nekoramu yekuparadzanisa.Mazhinji ekumashure sinusoidal zviyero zvinogadzirwa nekupfuura injector uye capillary column pakati peiyo static mixer uye UV detector.Paunenge uchiongorora chiyero chechiratidzo-ku-ruzha uye/kana kuongorora chimiro chepamusoro, masisitimu ehurongwa anoratidzwa muMufananidzo 5.
Mufananidzo 4. Plot yekusanganisa kunyatsoenderana nekuvhenganisa vhoriyamu yemhando ye static mixers.Kusachena kwedzidziso kunotevera maitiro akafanana neyeyedzo yekusachena data inosimbisa chokwadi cheiyo CFD simulations.
Iyo HPLC sisitimu yakashandiswa pabvunzo iyi yaive Agilent 1100 Series HPLC ine UV detector inodzorwa nePC inomhanyisa Chemstation software.Tafura 1 inoratidza yakajairika tuning mamiriro ekuyera musanganisi kunyatsoshanda nekutarisa basic sinusoids mune mbiri nyaya zvidzidzo.Miedzo yekuedza yakaitwa pamienzaniso miviri yakasiyana yezvinonyungudutsa.Izvo zviviri zvinonyungudutsa zvakasanganiswa kana 1 yaive solvent A (20 mM ammonium acetate mumvura deionized) uye solvent B (80% acetonitrile (ACN) / 20% deionized mvura).MuChikamu chechipiri, solvent A yaiva mhinduro ye 0.05% acetone (label) mumvura yakasvibiswa.Solvent B musanganiswa we80/20% methanol nemvura.Muchiitiko che 1, pombi yakagadzirirwa kuyerera kwe 0.25 ml / min kusvika 1.0 ml / min, uye kana 2, pombi yakaiswa kune imwe nguva yekuyerera kwe 1 ml / min.Muzviitiko zviviri izvi, chiyero chemusanganiswa wezvinonyungudutsira A uye B chaiva 20% A / 80% B. Iyo detector yakagadzirirwa 220 nm munyaya 1, uye iyo yakanyanya kutorwa yeacetone muchiitiko 2 yakagadzirirwa kusvika kureba kwe265 nm.
Tafura 1. HPLC Configurations for Cases 1 uye 2 Nyaya 1 Nyaya 2 Pump Speed ​​​​0.25 ml/min kusvika 1.0 ml/min 1.0 ml/min Solvent A 20 mM ammonium acetate mumvura yakasvibiswa 0.05% Acetone mumvura yakasvibiswa Solvent B 80% ACN 80% Aceto ACN (Aceto 80% ACN) 20% deionized mvura Solvent ratio 20% A / 80% B 20% A / 80% B Detector 220 nm 265 nm
Mupunga.6. Zvirongwa zvemusanganiswa sine mafungu akayerwa pamberi uye mushure mekuisa yakaderera-pass sefa kuti abvise baseline drift zvikamu zvechiratidzo.
Mufananidzo 6 muenzaniso wakajairwa weruzha rwekutanga rwakasanganiswa muNyaya 1, inoratidzwa seinodzokorora sinusoidal pateni yakaiswa pamusoro pekutanga kudonha.Baseline Drift inononoka kuwedzera kana kudzikira kwechiratidzo chekumashure.Kana iyo sisitimu isingatenderwe kuenzana kwenguva yakakwana, inowanzodonha, asi inodonha zvisizvo kunyangwe iyo sisitimu yakanyatsogadzikana.Iyi yekutanga kudhiraivha kunowedzera kuwedzera kana sisitimu iri kushanda mumawere gradient kana yakakwira kumashure kumanikidza mamiriro.Kana iyi yekutanga kudonha iripo, zvinogona kunetsa kuenzanisa mibairo kubva kumuenzaniso kuenda kumuenzaniso, iyo inogona kukurirwa nekuisa yakaderera-pass sefa kune yakaomeswa data kusefa iyi yakaderera-frequency misiyano, nekudaro ichipa oscillation plot ine flat baseline.Pamusoro pemuonde.Mufananidzo 6 unoratidzawo chirongwa chemusanganisi's baseline ruzha mushure mekushandisa yakaderera-pass sefa.
Mushure mekupedza kuenzanisa kweCFD uye kuyedza kwekutanga kuyedza, matatu akapatsanurwa static mixers akazogadzirwa pachishandiswa zvemukati zvinotsanangurwa pamusoro nemavhoriyamu matatu emukati: 30 µl, 60 µl uye 90 µl.Iri renji rinovhara huwandu hwemavhoriyamu uye kusanganisa kuita kunodiwa kune yakaderera analyte HPLC maapplication uko kwakagadziridzwa kusanganisa uye kuderera kupararira kunodiwa kuburitsa yakaderera amplitude baselines.Pamusoro pemuonde.7 inoratidza basic sine wave zviyero zvakawanikwa payedzo system yeMuenzaniso 1 (acetonitrile uye ammonium acetate sematracer) ine mavhoriyamu matatu eiyo static mixers uye pasina musanganiswa akaiswa.Mamiriro ekuedza ekuedza kwemigumisiro yakaratidzwa muMufananidzo 7 yakaitwa nguva dzose mumatambudziko ose e4 maererano nehurongwa hunotsanangurwa muTebhu 1 pahuwandu hwekuyerera kwe 0.5 ml / min.Isa kukosha kwekubvisa kune datasets kuti igone kuratidzwa padivi nepadivi pasina chiratidzo chinopindirana.Offset haikanganisi amplitude yechiratidzo chinoshandiswa kutonga danho rekuita remusanganisi.Avhareji sinusoidal amplitude isina musanganisi yaive 0.221 mAi, nepo amplitudes eiyo static Mott mixers pa30 µl, 60 µl, uye 90 µl yakadonha kusvika 0.077, 0.017, uye 0.004 mAi, zvichiteerana.
Mufananidzo 7. HPLC UV Detector Signal Offset vs. Time for Case 1 (acetonitrile ine ammonium acetate indicator) inoratidza solvent mixing pasina mixer, 30 µl, 60 µl uye 90 µl Mott mixers inoratidza kusanganiswa kwakavandudzwa (pasi pasi chiratidzo amplitude) sehuwandu hwehuwandu hwe static mixer.(chaiyo data offsets: 0.13 (hapana musanganisi), 0.32, 0.4, 0.45mA yekuratidzira zvirinani).
Iyo data inoratidzwa mufig.8 dzakafanana nemuna Fig. 7, asi panguva ino dzinosanganisira migumisiro yevatatu vanowanzoshandiswa HPLC static mixers ine mukati mavhoriyamu e50 µl, 150 µl uye 250 µl.Mupunga.Mufananidzo 8. HPLC UV Detector Signal Offset versus Time Plot for Case 1 (Acetonitrile uye Ammonium Acetate sezviratidzo) ichiratidza kusanganiswa kwe solvent pasina static mixer, mutsva mutsva weMott static mixers, uye vatatu vanogadziriswa (chaiyo data offset ndeye 0.1 (isina musanganisi), 0.60.8, 0.60. 9 mA zvichiteerana kuitira zvirinani kuratidza maitiro).Kudzikiswa kwepesenti ye base sine wave inoverengerwa nereshiyo yeamplitude ye sine wave kune amplitude pasina musanganisi akaiswa.Iyo yakayerwa sine wave attenuation percentages yeCase 1 ne2 yakanyorwa muTable 2, pamwe nemukati mavhoriyamu emusanganiswa static musanganiswa uye manomwe akajairwa musanganiswa anowanzo shandiswa muindasitiri.Iyo data iri muFigure 8 ne9, pamwe nemaverengero akaiswa muTable 2, anoratidza kuti Mott Static Mixer inogona kupa kusvika 98.1% sine wave attenuation, inopfuura nekure kuita kweyakajairwa HPLC musanganisi pasi pemamiriro aya ebvunzo.Mufananidzo 9. HPLC UV detector signal offset versus time plot for case 2 (methanol uye acetone se tracers) ichiratidza pasina static mixer (yakasanganiswa), mutsva mutsva weMott static mixers uye maviri akajairwa mixers (chaiyo data offsets ndeye 0, 11 (pasina musanganisi. ), 0.22, 0, 0 mA kuratidza zviri nani.Vanomwe vanomwe vaiwanzo shandiswa musanganiswa muindasitiri vakaongororwawo.Izvi zvinosanganisira ma mixer ane matatu akasiyana emukati mavhoriyamu kubva kukambani A (yakasarudzwa Mixer A1, A2 uye A3) uye kambani B (yakasarudzwa Mixer B1, B2 uye B3).Kambani C yakaratidza saizi imwe chete.
Tafura 2. Static Mixer Inokurudzira Hunhu uye Internal Volume Static Mixer Case 1 Sinusoidal Recovery: Acetonitrile Test (Kushanda) Nyaya 2 Sinusoidal Recovery: Methanol Water Test (Kubudirira) Internal Volume (µl) Hapana Musanganisi - - 0 Mott% 6% 30 9 Mott 30 . 1.3% 60 Mott 90 98.1% 97.5% 90 Mixer A1 66.4% 73.7% 50 Mixer A2 89.8% 91.6% 150 Mixer A3 92.2% 94.5% 250 Mixer B1 45%. 370 Musanganisi C 97.2% 97.4% 250
Ongororo yezvabuda muMufananidzo 8 neTafura 2 inoratidza kuti 30 µl Mott static mixer ine musanganiswa wakafanana neA1 musanganisi, kureva 50 µl, zvisinei, iyo 30 µl Mott ine 30% shoma mukati.Kana tichienzanisa iyo 60 µl Mott musanganisi ne150 µl yemukati vhoriyamu A2 musanganisi, pakave nekuvandudzika kushoma mukusanganisa kugona kwe92% maringe ne89%, asi zvinonyanya kukosha, iyi nhanho yepamusoro yekusanganisa yakawanikwa pa1/3 yevhoriyamu yemusanganisi.musanganiswa wakafanana A2.Kuita kwe90 µl Mott musanganisi kwakatevera maitiro akafanana nea3 musanganisi ane vhoriyamu yemukati ye250 µl.Kuvandudzwa kwekusanganisa kuita kwe98% uye 92% kwakaonekwawo nekuderedzwa kwe3-kupeta mukati mevhoriyamu.Migumisiro yakafanana uye kuenzanisa zvakawanikwa kune vasanganisi B uye C. Somugumisiro, mutsva mutsva we static mixers Mott PerfectPeak TM inopa hukuru hwepamusoro hwekusanganiswa kupfuura hunofananidzwa nemakwikwi emakwikwi, asi nehuwandu hwemukati wemukati, huchipa huri nani huri shure uye chiyero chakanaka chechiratidzo-ku-noise, zviri nani senitivity Analyte, peak shape uye peak resolution.Mafambiro akafanana mukusanganisa kunyatsoshanda akaonekwa mune zvese Case 1 uye Nyaya 2 zvidzidzo.Panyaya yechipiri, bvunzo dzakaitwa pachishandiswa (methanol uye acetone sezviratidzo) kuenzanisa kusanganisa kugona kwe60 ml Mott, musanganisi anofananidzwa A1 (yemukati vhoriyamu 50 µl) uye inofananidzwa musanganisi B1 (yemukati vhoriyamu 35 µl)., kushanda kwaive kwakashata pasina musanganiswa wakaiswa, asi wakashandiswa pakuongorora kwekutanga.Iyo 60 ml Mott musanganiswa yakaratidza kuve yakanakisa musanganisi muboka rebvunzo, ichipa 90% yekuwedzera mukusanganisa kugona.Musanganiswa A1 wakafananidzwa wakaona 75% kuvandudzwa mukusanganisa kushanda zvakanaka kunoteverwa ne45% kuvandudzwa mune inofananidzwa B1 musanganiswa.Yekutanga sine wave kuderedza bvunzo ine kuyerera kweyero yakaitwa pane akatevedzana emusanganiswa pasi pemamiriro akafanana neiyo sine curve bvunzo muCase 1, paine chete kuyerera kwakachinja.Iyo data yakaratidza kuti muhuwandu hwekuyerera kwemazinga kubva ku0.25 kusvika 1 ml / min, kuderera kwekutanga mu sine wave kwakaramba kwakangofanana kune ese matatu mavheji mavhoriyamu.Kune maviri madiki vhoriyamu mixers, kune kudiki kuwedzera kwe sinusoidal contraction sezvo kuyerera kunoderera, izvo zvinotarisirwa nekuda kwekuwedzera kwekugara nguva yezvinonyungudutsa mumusanganisi, zvichibvumira kuwedzera kusanganisa kusanganisa.Kubviswa kweiyo sine wave kunotarisirwa kuwedzera sezvo kuyerera kunowedzera kuderera.Nekudaro, kune yakakura kwazvo musanganiswa vhoriyamu ine yepamusoro sine wave base base attenuation, iyo sine wave base base attenuation yakaramba isina kushanduka (mukati meyedzo yekusavimbika kwekuyedza), ine kukosha kubva pa95% kusvika 98%.Mupunga.10. Basic attenuation ye sine wave versus flow rate in case 1. Muedzo wakaitwa pasi pemamiriro akafanana neine test ine variable flow rate, injecting 80% ye80/20 musanganiswa weacetonitrile nemvura uye 20% ye20 mM ammonium acetate.
Iyo ichangobva kugadzirwa mhando ye patented PerfectPeak TM inline static mixers ine matatu emukati mavhoriyamu: 30 µl, 60 µl uye 90 µl inovhara vhoriyamu uye kusanganisa kuita kwekuita kunodiwa kune yakawanda HPLC ongororo inoda yakavandudzwa musanganiswa uye yakaderera kupararira pasi.Iyo nyowani static musanganiswa inowana izvi nekushandisa nyowani 3D yekudhinda tekinoroji kugadzira yakasarudzika 3D chimiro chinopa yakagadziridzwa hydrodynamic static musanganiswa pamwe nepamusoro pezana kuderedzwa kweruzha rwepasi pachikamu chevhoriyamu yemusanganiswa wemukati.Kushandisa 1/3 yevhoriyamu yemukati yemuvhenganisi yakajairika inoderedza ruzha rwepasi ne98%.Misanganisi yakadai inoumbwa neakabatana-mativi matatu-eyekuyerera chiteshi ane akasiyana-chikamu-chikamu nzvimbo uye akasiyana nzira kureba sezvo mvura inoyambuka yakaoma geometric zvipingamupinyi mukati.Mhuri itsva yevasanganisi vane static inopa kushanda kwakavandudzwa pamusoro pemakwikwi emakwikwi, asi nehuwandu hwemukati, zvichiita kuti zvive nani zviratidzo-ku-ruzha reshiyo uye kuderera kwehuwandu hwemiganhu, pamwe nekuvandudzwa kwepamusoro peak shape, kushanda zvakanaka uye kugadzirisa kwekunzwa kwepamusoro.
Muchinyorwa ichi Chromatography - Yakanakira zvakatipoteredza RP-HPLC - Kushandisa core-shell chromatography kutsiva acetonitrile neisopropanol mukuongorora nekucheneswa - New gasi chromatograph ye…
Business Center International Labmate Limited Oak Court Sandridge Park, Porters Wood St Albans Hertfordshire AL3 6PH United Kingdom